Information on EC 1.4.3.2 - L-amino-acid oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.4.3.2
-
RECOMMENDED NAME
GeneOntology No.
L-amino-acid oxidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
mechanism
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
mechanism
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
ping-pong mechanism with binary complexes, derived from kinetic data
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
mechanism
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
allosteric effects, activation energy
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
mechanism
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
reaction mechanism. Catalytic amino acids are H223 in two conformations, G464 and R322
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
hydride transfer mechanism
-
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidative deamination
-
-
-
-
redox reaction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
-
arginine degradation VIII (arginine oxidase pathway)
-
Cysteine and methionine metabolism
-
Isoquinoline alkaloid biosynthesis
-
Metabolic pathways
-
Phenylalanine metabolism
-
Phenylalanine, tyrosine and tryptophan biosynthesis
-
Tryptophan metabolism
-
Tyrosine metabolism
-
Valine, leucine and isoleucine degradation
-
SYSTEMATIC NAME
IUBMB Comments
L-amino-acid:oxygen oxidoreductase (deaminating)
A flavoprotein (FAD).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
APIT
Q6T277
-
aromatic L-amino acid oxidase
-
-
-
-
Balt-LAAO-I
-
-
Bpir-LAOO-1
-
-
Dolabellanin
-
-
escapin
Aplysia californica Cooper 1863
Q6IWZ0
-
-
IL4I1
-
interleukin 4 induced gene 1
IL4I1
-
-
Il4i1 protein
-
secreted L-phenylalanine oxidase, physiologically expressed by myeloid cells, gene expression in primary mediastinal B-cell lymphoma
Il4i1 protein
-
-
ink toxin 1
Q6T277
-
Interleukin Four Induced Gene 1 protein
-
first described in the mouse, characterized in human B cells, expression is restricted to lymphoid tissues and induced by interleukin-4
L-AAO
Q6IWZ0
-
L-AAO
Pseudoalteromonas flavipulchra C2
-
-
-
L-AAO
Rhodococcus sp. AIU Z-35-1
-
-
-
L-amino acid oxidase
-
-
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
Hypocrea lixii ETS 323
-
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
-
L-amino acid oxidase
-
-
L-amino acid oxidase
-
-
L-amino acid: O2 oxidoreductase
-
-
L-amino acid:O2 oxidoreductase
-
-
-
-
L-amino acid:O2 oxidoreductase (deaminating)
-
-
L-aminooxidase
-
-
-
-
L-phenylalanine oxidase
-
-
LAAO
-
-
-
-
LAAO
Q6IWZ0
-
LAAO
Aplysia californica Cooper 1863
Q6IWZ0
-
-
LAAO
A8QL52
-
LAAO
-
-
LAAO
Hypocrea lixii ETS 323
-
-
-
LAAO
-
-
LAAO
A8QL58
-
LAAO
-
-
LAAO
A8QL50
-
LAAO-I
Q6TGQ9
-
LAAO-I
Q6TGQ8
-
LAO
-
-
-
-
LAO
Hebeloma cylindrosporum CBS 558.96
-
-
-
LAO
Hebeloma sp., Laccaria bicolor
-
-
LAO
Laccaria bicolor S238N
-
-
-
LAO
Pseudoalteromonas luteoviolacea CPMOR-1, Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea NCIMB 1893T
-
-
-
M-LAO
Q90W54
-
ophio-amino-acid oxidase
-
-
-
-
Sebastes schlegeli antibacterial protein
A1IGW6
-
Sebastes schlegelii antibacterial protein
-
-
SSAP
A1IGW6
-
toxophallin
-
-
CAS REGISTRY NUMBER
COMMENTARY
9000-89-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
cottonmouth moccasin
-
-
Manually annotated by BRENDA team
sea hare, strain Cooper 1863 collected in California
Swissprot
Manually annotated by BRENDA team
strain Cooper 1863 collected in California
Swissprot
Manually annotated by BRENDA team
Aplysia californica Cooper 1863
strain Cooper 1863 collected in California
Swissprot
Manually annotated by BRENDA team
-
Q6T277
SwissProt
Manually annotated by BRENDA team
Bacillus carotarum
2Pfa
-
-
Manually annotated by BRENDA team
Bacillus carotarum 2Pfa
2Pfa
-
-
Manually annotated by BRENDA team
Bothrops sp.
venom
-
-
Manually annotated by BRENDA team
llected from Guangxi Province, P.R. China
-
-
Manually annotated by BRENDA team
; expression in Pichia pastoris
-
-
Manually annotated by BRENDA team
Corynebacterium sp. A20
A20
-
-
Manually annotated by BRENDA team
eastern diamondback rattlesnake, multiple electrophoretic components
-
-
Manually annotated by BRENDA team
Crotalus terrificus terrificus
-
-
-
Manually annotated by BRENDA team
strain CBS 558.96
-
-
Manually annotated by BRENDA team
Hebeloma cylindrosporum CBS 558.96
strain CBS 558.96
-
-
Manually annotated by BRENDA team
Hebeloma sp.
strains SE5, F-NB01 , F-RS01, R-RS01 and SIV
-
-
Manually annotated by BRENDA team
lymphoid and non lymphoid malignancies, lymphoma cell lines L428, KM-H2, and SU-DHL-4
-
-
Manually annotated by BRENDA team
Hypocrea lixii ETS 323
-
-
-
Manually annotated by BRENDA team
strain S238N
-
-
Manually annotated by BRENDA team
Laccaria bicolor S238N
strain S238N
-
-
Manually annotated by BRENDA team
milk
Uniprot
Manually annotated by BRENDA team
isozymes MPLAO1, MPLAO2, and MPLAO3
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
inducible, expression in cells derepressed for nitrogen in presence of an amino acid, intracellular and extracellular location
-
-
Manually annotated by BRENDA team
no activity in Acinetobacter anitratum
-
-
-
Manually annotated by BRENDA team
no activity in Alcaligenes faecalis
-
-
-
Manually annotated by BRENDA team
no activity in Anabaena variabilis ATCC 29413
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis subsp. subtilis
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis subsp. subtilis NCIB 3610
-
-
-
Manually annotated by BRENDA team
no activity in Diplococcus pneumoniae
-
-
-
Manually annotated by BRENDA team
no activity in Enterobacter aerogenes
-
-
-
Manually annotated by BRENDA team
no activity in Escherichia coli
-
-
-
Manually annotated by BRENDA team
no activity in Klebsiella pneumoniae
-
-
-
Manually annotated by BRENDA team
no activity in Micrococcus tetragenus
-
-
-
Manually annotated by BRENDA team
no activity in Moraxella catarrhalis
-
-
-
Manually annotated by BRENDA team
no activity in Mycobacterium phlei
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus MED 4
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus MIT 9211
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus MIT 9312
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus MIT 9313
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus NATL 2A
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus SS 120
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas aeruginosa
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida T1
-
-
-
Manually annotated by BRENDA team
no activity in Serratia marcescens
-
-
-
Manually annotated by BRENDA team
no activity in Shigella flexneri
-
-
-
Manually annotated by BRENDA team
no activity in Staphylococcus aureus
-
-
-
Manually annotated by BRENDA team
no activity in Staphylococcus epidermidis
-
-
-
Manually annotated by BRENDA team
no activity in Streptococcus viridans
-
-
-
Manually annotated by BRENDA team
no activity in Synechococcus sp.
-
-
-
Manually annotated by BRENDA team
no activity in Synechococcus sp. CC9902
-
-
-
Manually annotated by BRENDA team
no activity in Synechococcus sp. JA-2-3B'a(2-13)
-
-
-
Manually annotated by BRENDA team
no activity in Synechococcus sp. JA-3-3Ab
-
-
-
Manually annotated by BRENDA team
no activity in Synechococcus sp. RS9917
-
-
-
Manually annotated by BRENDA team
no activity in Synechococcus sp. WH 8102
-
-
-
Manually annotated by BRENDA team
no activity in Thermosynechococcus elongatus BP-1
-
-
-
Manually annotated by BRENDA team
-
A8QL50
UniProt
Manually annotated by BRENDA team
king cobra
-
-
Manually annotated by BRENDA team
30 different strains
-
-
Manually annotated by BRENDA team
Taiwan habu snake
-
-
Manually annotated by BRENDA team
10 different strains
-
-
Manually annotated by BRENDA team
PCM 1298
-
-
Manually annotated by BRENDA team
Providencia sp. PCM 1298
PCM 1298
-
-
Manually annotated by BRENDA team
isolated from the encrusting pore coral Montipora aequituberculata from the Kenting coast of Southern Taiwan
-
-
Manually annotated by BRENDA team
Pseudoalteromonas flavipulchra C2
isolated from the encrusting pore coral Montipora aequituberculata from the Kenting coast of Southern Taiwan
-
-
Manually annotated by BRENDA team
strain CPMOR-1; strain CPMOR-2; strain NCIMB 1893T
-
-
Manually annotated by BRENDA team
Pseudoalteromonas luteoviolacea CPMOR-1
strain CPMOR-1
-
-
Manually annotated by BRENDA team
Pseudoalteromonas luteoviolacea CPMOR-2
strain CPMOR-2
-
-
Manually annotated by BRENDA team
Pseudoalteromonas luteoviolacea NCIMB 1893T
strain NCIMB 1893T
-
-
Manually annotated by BRENDA team
DSM 43250; DSM 43250, one of the best L-AAO producer
SwissProt
Manually annotated by BRENDA team
lao-gene; DSM 43250 strain
SwissProt
Manually annotated by BRENDA team
Rhodococcus sp. AIU Z-35-1
-
-
-
Manually annotated by BRENDA team
-
A1IGW6
SwissProt
Manually annotated by BRENDA team
Synechococcus cedrorum PCC 6908
-
-
-
Manually annotated by BRENDA team
Synechococcus leopoliensis (blue-green algae)
-
-
Manually annotated by BRENDA team
captured in northwest Bulgaria, five LAAO isozymes
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
the enzyme induces in vitro platelet aggregation, which may be due to H2O2 production, the addition of catalase completely inhibits the aggregation effect. It also shows cytotoxicity towards several cancer cell lines: HL60, Jurkat, B16F10 and PC12 causing apoptosis. The cytotoxicity activity is abolished by catalase
physiological function
B5U982
the antibacterial LAO isozymes may be involved in the innate immunity of the great sculpin Myoxocephalus polyacanthocephalus skin
physiological function
-
Akbu-LAAO shows apparent anti-aggregation effects on human and rabbit platelets and exhibits a strong bacteriostasis effect on Staphylococcus aureus, 18fold that of cephalosporin C
physiological function
-
serum LAO is involved in innate immunity in the rockfish body, it exhibits a broad antibacterial activity against Gram positive and negative bacteria, most potently against Aeromonas hydrophila and Aeromonas salmonicida with a minimum inhibitory concentration of 0.000078 mg/ml
physiological function
-
venom LAAO is very effective in inhibiting the two Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis with MICs of 0.000006 mM and 0.000012 mM, respetively. LAAO is only moderately effective against three Gram-negative bacteria tested, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, overview. Binding to bacteria is important for the potent antibacterial activity of the enzyme
physiological function
-
the enzyme shows from strain C2 shows potent antibacterial activity against several methicillin-resistant Staphylococcus aureus strains, antibacterial spectrum, overview
physiological function
-
toxophallin induces chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis. Toxophallin uses a caspase-independent pathway of apoptosis induction
physiological function
-
the enzyme acts against waterborne Gram-negative bacteria and functions locally as a humoral defense factor in Sebastes schlegeli skin and gills preventing pathogenic bacterial invasion
physiological function
-
the enzyme interacts with bacteria to cause membrane permeabilization. Reactive oxidative species accumulation triggers forms of cell damage, including lipid peroxidation and DNA strand breakage that results in bacterial growth inhibition
physiological function
-
IL41I inhibits bacterial growth. The antibiotic action of IL4I1 is mainly due to H2O2 and NH3 production whereas its L-amino acid oxidase activity does not sufficiently deplete the medium of Phe or Trp to inhibit bacterial growth
physiological function
-
the enzyme induces cytotoxicity on AGS cell line (gastric adenocarcinoma) and on MCF-7 cell line (breast adenocarcinoma). It presents antiparasitic activity on Leishmania brasiliensis, but Trypanosoma cruzi is resistant to this enzyme
physiological function
-
the enzyme inhibits in a dose-dependent manner the growth of Staphylococcus aureus and Acinetobacter baumannii, however, against Escherichia coli the enzyme does not show inhibitory capacity and does not exhibit cytotoxic activity on the mouse myoblast cell line C2C12 and on peripheral blood mononuclear cell
physiological function
Hypocrea lixii ETS 323
-
the enzyme interacts with bacteria to cause membrane permeabilization. Reactive oxidative species accumulation triggers forms of cell damage, including lipid peroxidation and DNA strand breakage that results in bacterial growth inhibition
-
physiological function
Pseudoalteromonas flavipulchra C2
-
the enzyme shows from strain C2 shows potent antibacterial activity against several methicillin-resistant Staphylococcus aureus strains, antibacterial spectrum, overview
-
additional information
-
toxophallin is a highly cytotoxic protein, but differs distinctly from the other death cap toxic protein phallolysin. The interaction of toxophallin with target cells is not mediated by a specific cell surface receptor
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,1,2,2-tetrafluoroethyl)-L-cysteine + H2O + O2
2-oxo-3-[(1,1,2,2-tetrafluoroethyl)sulfanyl]propanoic acid + NH3 + H2O2
show the reaction diagram
-
80% of the activity with L-methionine
-
-
?
3,4-dihydroxyphenyl-L-Ala + H2O + O2
3-(3,4-dihydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
alpha-hydroxyisocaproic acid + H2O + O2
2-oxo-4-methylpentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
alpha-hydroxyvaleric acid + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
an L-amino acid + H2O + O2
a 2-oxo-acid + NH3 + H2O2
show the reaction diagram
-
L-amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective imino acids with concomitant hydrolysis to alpha-keto acids, together with the production of ammonia and hydrogen peroxide
-
-
?
DL-3-(1-pyridyl)-alanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
24% of the activity compared to L-alanine
-
?
DL-3-(1-pyridyl)alanine + H2O + O2
3-(1-pyridyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
24% of the activity with L-Ala
-
-
?
DL-4-fluorophenylalanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
25% of the activity compared to L-alanine
-
?
DL-4-fluorophenylalanine + H2O + O2
(4-fluorophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
25% of the activity with L-Ala
-
-
?
DL-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
DL-beta-phenylserine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
8% of the activity with L-Ala
-
-
?
DL-homophenylalanine + H2O + O2
2-oxo-4-phenylbutanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
50% of the activity compared to L-alanine
-
?
DL-homophenylalanine + H2O + O2
2-oxo-4-phenylbutyric acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
50% of the activity with L-Ala
-
-
?
DL-leucine + H2O + O2
2-oxo-4-methylpentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
DL-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
DL-norleucine + H2O + O2
alpha-ketocaproate + NH3 + H2O2
show the reaction diagram
Q8VPD4
73% of the activity compared to L-alanine
-
?
DL-norleucine + H2O + O2
alpha-ketocaproate + NH3 + H2O2
show the reaction diagram
Q8VPD4
73% of the activity with L-Ala
-
-
?
DL-norleucine + H2O + O2
2-oxocaproate + NH3 + H2O2
show the reaction diagram
-, Q90W54
-
-
-
?
DL-norleucine + H2O + O2
2-oxocaproate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
DL-O-methylserine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
99% of the activity compared to L-alanine
-
?
DL-O-methylserine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
99% of the activity with L-Ala
-
-
?
DL-phenylseine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
8% of the activity compared to L-alanine
-
?
DL-threonine + H2O + O2
2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
DL-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
DL-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
show the reaction diagram
-
not
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
show the reaction diagram
-
not
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
glycine + H2O + O2
glyoxylate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
-
glycine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
hydroxyisovaleric acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-2,3-diaminopropionic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
71% of the activity compared to L-alanine
-
?
L-2,3-diaminopropionic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
71% of the activity with L-Ala
-
-
?
L-2,4-diaminobutyric acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
100% of the activity compared to L-alanine
-
?
L-2,4-diaminobutyric acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
as active as L-Ala
-
-
?
L-2-4,4,dimethylvaleric acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
46% of the activity compared to L-alanine
-
?
L-2-amino-4,4-dimethylhexanoic acid + H2O + O2
4,4-dimethyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
44% of the activity with L-Ala
-
-
?
L-2-amino-4,4-dimethylvaleric acid + H2O + O2
4,4-dimethyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
46% of the activity with L-Ala
-
-
?
L-2-amino-5,5-dimethylhexanoic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
44% of the activity compared to L-alanine
-
?
L-2-chlorophenylalanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
24% of the activity compared to L-alanine
-
?
L-2-chlorophenylalanine + H2O + O2
(2-chlorophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
24% of the activity with L-Ala
-
-
?
L-3-(1-naphthyl)alanine + H2O + O2
3-(1-naphthyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
109% of the activity with L-Ala
-
-
?
L-3-(naphthyl)alanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
109% of the activity compared to L-alanine
-
?
L-4-aminophenylalanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
16% of the activity compared to L-alanine
-
?
L-4-aminophenylalanine + H2O + O2
(4-aminophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
16% of the activity with L-Ala
-
-
?
L-4-chlorophenylalanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
9% of the activity compared to L-alanine
-
?
L-4-chlorophenylalanine + H2O + O2
(4-chlorophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
9% of the activity with L-Ala
-
-
?
L-4-nitrophenylalanine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
30% of the activity compared to L-alanine
-
?
L-4-nitrophenylalanine + H2O + O2
(4-nitrophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
30% of the activity with L-Ala
-
-
?
L-Ala + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
-
-
-
?
L-Ala + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ala + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Ala + H2O + O2
2-oxo-3-propanoate + NH3 + H2O2
show the reaction diagram
-
the substrate is not effectively oxidized
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4, -
-
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
highly enantioselective enzyme with a broad substrate specificity
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4, -
highly enantioselective enzyme with a broad substrate specificity
-
?
L-albizziin + H2O + O2
alpha-keto-beta-ureidopropionic acid + NH3 + H2O2
show the reaction diagram
-
L-alpha-amino-beta-ureidopropionic acid
-
-
?
L-alpha-aminobutyric acid + H2O + O2
alpha-ketobutyrate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-alpha-aminobutyric acid + H2O + O2
alpha-ketobutyrate + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
O09046
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Crotalus terrificus terrificus
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
snake
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Bothrops sp.
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
all amino acids except cysteine
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
other electron acceptors: methylene blue
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
first step in reaction sequence of formation of phenylacetic acid from L-phenylalanine
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
functional in photosynthetic and respiratory activities as well as in L-arginine degradation
-
-
-
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
functional in photosynthetic and respiratory activities as well as in L-arginine degradation
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
utilization of amino acids as nitrogen source
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
operates as scavanger of ammonium from extracellular amino acids
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
bactericidal action of L-amino acid oxidase
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
involved in the water-splitting reaction of photosystem II
-
-
-
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
involved in the water-splitting reaction of photosystem II
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
O09046
responsible for killing bacteria in the mammary gland
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
snake
-
biological functions of snake venom enzymes
-
-
-
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
cell death induced by the enzyme is of two types, one non-apoptotic and the other apoptotic. The effect is not dependent on the acute toxicity of H2O2
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Corynebacterium sp. A20
-
-, utilization of amino acids as nitrogen source
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Providencia sp. PCM 1298
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum 2Pfa
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Arg + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Arg + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Arg + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
70% of the activity with L-Ala
-
-
?
L-Arg + H2O + O2
5-carbamidamido-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
the substrate is not effectively oxidized
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-, Q6T277
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
good substrate
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
70% of the activity compared to L-alanine
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
low enzyme activity
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Synechococcus elongatus PCC 7942, Synechococcus cedrorum PCC 6908
-
best substrate, 100% substrate specificity
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
P72346
best substrate, 100% substrate specificity
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
about 10% activity compared to L-methionine
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea CPMOR-1
-
low enzyme activity
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum 2Pfa
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea NCIMB 1893T
-
low enzyme activity
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
show the reaction diagram
Rhodococcus sp. AIU Z-35-1
-
-
-
-
?
L-arginine + H2O + O2
5-carbamidamido-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
6% activity compared to L-lysine
-
-
?
L-arginine + H2O + O2
5-carbamidamido-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
about 20% activity compared to L-methionine
-
-
?
L-arginine + H2O + O2
5-carbamidamido-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
6% activity compared to L-lysine
-
-
?
L-Asn + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Asp + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
11% of the activity with L-Ala
-
-
?
L-Asp + H2O + O2
alpha-ketosuccinamic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
97% of the activity with L-Ala
-
-
?
L-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-asparagine + H2O + O2
alpha-ketosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-asparagine + H2O + O2
alpha-ketosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-asparagine + H2O + O2
alpha-ketosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
very low activity
-
-
?
L-asparagine + H2O + O2
alpha-ketosuccinamic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
97% of the activity compared to L-alanine
-
?
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
Q8VPD4
11% of the activity compared to L-alanine
-
?
L-citrulline + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
74% of the activity compared to L-alanine
-
?
L-citrulline + H2O + O2
N5-carbamoyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
74% of the activity with L-Ala
-
-
?
L-citrulline + H2O + O2
2-oxo-5-ureidovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-cysteic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
34% of the activity compared to L-alanine
-
?
L-cysteic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
34% of the activity with L-Ala
-
-
?
L-cysteine + H2O + O2
2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-cysteine + H2O + O2
2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
show the reaction diagram
Providencia rettgeri, Crotalus terrificus terrificus
-
-
-
-
-
L-cysteine + H2O + O2
2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-cysteine + H2O + O2
2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
show the reaction diagram
-
40% of the activity with L-methionine
-
-
?
L-cysteine + H2O + O2
2-oxo-3-thiopropanoate + NH3 + H2O2
show the reaction diagram
-
about 55% activity compared to L-methionine
-
-
?
L-cystine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
very low activity
-
-
?
L-cystine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
104% of the activity with L-Ala
-
-
?
L-cystine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
35% of the activity with L-methionine
-
-
?
L-cystine + H2O + O2
2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
104% of the activity compared to L-alanine
-
?
L-cystine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
L-Dopa + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
about 10% of the activity with L-Phe
-
-
?
L-Gln + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Gln + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
Q8VPD4
52% of the activity with L-Ala
-
-
?
L-Glu + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Glu + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Glu + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
Q8VPD4
52% of the activity with L-Ala
-
-
?
L-glutamate + H2O + O2
2-oxoglutaric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
Q8VPD4
52% of the activity compared to L-alanine
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
high enzyme activity
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-glutamine + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
very low activity
-
-
?
L-glutamine + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
10% of the activity with L-methionine
-
-
?
L-glutamine + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
high enzyme activity
-
-
?
L-glutamine + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
about 5% activity compared to L-methionine
-
-
?
L-glutamine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
52% of the activity compared to L-alanine
-
?
L-glutamine + H2O + O2
2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamine + H2O + O2
2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamine + H2O + O2
2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
show the reaction diagram
Rhodococcus sp. AIU Z-35-1
-
-
-
-
?
L-glutamine + H2O + O2
5-amino-2,5-dioxopentanoate + NH3 + H2O2
show the reaction diagram
-
about 11% activity compared to L-methionine
-
-
?
L-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
36% of the activity with L-Ala
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
Hebeloma sp.
-
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
A8QL50, -
-
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
36% of the activity compared to L-alanine
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
low enzyme activity
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
Synechococcus elongatus PCC 7942, Synechococcus cedrorum PCC 6908
-
poor substrate, less than 2% substrate specificity
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
P72346
poor substrate, less than 2% substrate specificity
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea CPMOR-1, Pseudoalteromonas luteoviolacea NCIMB 1893T
-
low enzyme activity
-
-
?
L-histidine + H2O + O2
2-oxo-4-imidazolepropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
2-oxo-4-imidazolepropionic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
about 19% activity compared to L-methionine
-
-
?
L-homocysteine + H2O + O2
4-thio-2-ketobutyric acid + NH3 + H2O2
show the reaction diagram
Providencia rettgeri, Crotalus terrificus terrificus
-
-
-
-
-
L-homocysteine + H2O + O2
4-thio-2-ketobutyric acid + NH3 + H2O2
show the reaction diagram
snake
-
-
-
-
?
L-homocysteine thiolactone + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
23% of the activity compared to L-alanine
-
?
L-homocysteine thiolactone + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
23% of the activity with L-Ala
-
-
?
L-homoserine + H2O + O2
2-oxo-4-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-homoserine lactone + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
66% of the activity compared to L-alanine
-
?
L-homoserine lactone + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
66% of the activity with L-Ala
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
Q4F867
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
Q8VPD4
45% of the activity with L-Ala
-
-
?
L-isoleucine + H2O + O2
alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-isoleucine + H2O + O2
alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-isoleucine + H2O + O2
alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
45% of the activity compared to L-alanine
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxo-pentanoate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
low enzyme activity
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
A8QL50, -
-
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
high catalytic activity
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q6TGQ9
high substrate activity
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
about 35% activity compared to L-methionine
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
about 68% activity compared to L-methionine
-
-
?
L-lactic acid + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q4F867
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
68% of the activity with L-Ala
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
LAAOs are flavoenzymes catalyzing the stereospecifically oxidative deamination of a wide range of L-amino acids
-
-
?
L-Leu + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Hebeloma sp.
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
A8QL50, -
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
good substrate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Bothrops sp.
-
high reaction rate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
no reaction with D-leucine
-
-
-
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
68% of the activity compared to L-alanine
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
high affinity for the substrate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
high enzyme activity
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q6TGQ9
high substrate activity, most specific substrate
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
one of the best substrates
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Laccaria bicolor S238N
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea CPMOR-1
-
high enzyme activity
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum 2Pfa
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea NCIMB 1893T
-
high enzyme activity
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Hebeloma cylindrosporum CBS 558.96
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylpentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylpentanoate acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
about 99% activity compared to L-methionine
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
about 97% activity compared to L-methionine
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
-
the enzymatic reaction likely forms an equilibrium mixture containing the linear form 6-amino-2-oxohexanoic acid, the cyclic imine D1-piperidine-2-carboxylic acid, the cyclic enamine D2-piperidine-2-carboxylic acid, possibly the linear enol 6-amino-2-hydroxy-hex-2-enoic acid, the alpha-dihydroxyacid 6-amino-2,2-dihydroxy-hexanoic acid, and the cyclic aminol 2-hydroxy-piperidine-2-carboxylic acid, identification and quantification by mass spectrometry and NMR, overview
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
55% of the activity with L-Ala
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
the ink of sea hares contains escapin, an L-amino acid oxidase that metabolizes L-lysine, thereby producing a mixture that kills microbes and deters attacking predators. This secretion contains H2O2, ammonia, and an equilibrium mixture of socalled escapin intermediate product, EIP-K, that includes 6-amino-2-oxohexanoic acid and several other molecules. Components of the equilibrium mixture react nonenzymatically with H2O2 to form escapin end product, i.e. EEP-K, which contains 2-aminovaleric acid and 2-valerolactam. The proportions of the components in this equilibrium mixture change with pH, and this is biologically important because the secretion has pH 5 when released but becomes pH 8 when fully diluted in seawater, overview
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Aplysia californica Cooper 1863
Q6IWZ0
-
-, the enzymatic reaction likely forms an equilibrium mixture containing the linear form 6-amino-2-oxohexanoic acid, the cyclic imine D1-piperidine-2-carboxylic acid, the cyclic enamine D2-piperidine-2-carboxylic acid, possibly the linear enol 6-amino-2-hydroxy-hex-2-enoic acid, the alpha-dihydroxyacid 6-amino-2,2-dihydroxy-hexanoic acid, and the cyclic aminol 2-hydroxy-piperidine-2-carboxylic acid, identification and quantification by mass spectrometry and NMR, overview
-
?
L-Lys + H2O + O2
6-amino-2-oxo-hexanoic acid + NH3 + H2O2
show the reaction diagram
B5U982
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
the substrate is not effectively oxidized
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Bacillus carotarum
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6T277
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
A1IGW6, -
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Hebeloma sp.
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
A8QL50, -
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
strictly specific for
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
55% of the activity compared to L-alanine
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
P72346
73% substrate specificity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Synechococcus cedrorum PCC 6908
-
81% substrate specificity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
82% substrate specificity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Laccaria bicolor S238N
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Rhodococcus sp. AIU Z-35-1
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
100% activity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
about 19% activity compared to L-methionine
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
100% activity
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
B5AR80
best substrate
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Q8VPD4
72% of the activity with L-Ala
-
-
?
L-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Bacillus carotarum
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Hebeloma sp.
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
A8QL50, -
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
preferred substrate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
good substrate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Bothrops sp.
-
high reaction rate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
100% activity
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Q8VPD4
72% of the activity compared to L-alanine
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
high affinity for the substrate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
high enzyme activity
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Q6TGQ9
high substrate activity
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
one of the best substrates
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Laccaria bicolor S238N
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea CPMOR-1
-
high enzyme activity
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Bacillus carotarum 2Pfa
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea NCIMB 1893T
-
high enzyme activity
-
-
?
L-methionine + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
100% activity
-
-
?
L-norleucine + H2O + O2
alpha-ketocaproate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-norleucine + H2O + O2
alpha-ketocaproate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
high catalytic activity
-
-
?
L-norvaline + H2O + O2
2-oxo-valeric acid + NH3 + NADH + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
L-norvaline + H2O + O2
2-oxo-valeric acid + NH3 + NADH + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-Orn + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
Q8VPD4
117% of the activity with L-Ala
-
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
Q8VPD4
117% of the activity compared to L-alanine
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
28% substrate specificity
-
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
Synechococcus cedrorum PCC 6908
-
35% substrate specificity
-
-
?
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
P72346
46% substrate specificity
-
-
?
L-ornithine + H2O + O2
?
show the reaction diagram
-
31% activity compared to L-lysine
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q4F867
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
53% of the activity with L-Ala
-
-
?
L-Phe + H2O + O2
2-oxo-3-phenylpropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Bacillus carotarum
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
good substrate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Bothrops sp.
-
high reaction rate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
53% of the activity compared to L-alanine
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
high affinity for the substrate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
high enzyme activity
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q6TGQ9
high substrate activity, most specific substrate
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
hydride transfer as the mechanism of dehydrogenation. The substrate-binding domain of LAAO is made up of residues 52-128, 230-238 and 315-422. The substrate-binding domain has almost the same topology as the snake venom LAAO
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
maximum catalytic efficiency for both isoforms
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
one of the best substrates
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
primary amino acid catabolized by IL4I1
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
about 55% activity compared to L-methionine
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Laccaria bicolor S238N
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea CPMOR-1
-
high enzyme activity
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Corynebacterium sp. A20
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Hypocrea lixii ETS 323
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Bacillus carotarum 2Pfa
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas luteoviolacea NCIMB 1893T
-
high enzyme activity
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Rhodococcus sp. AIU Z-35-1
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Hebeloma cylindrosporum CBS 558.96
-
-
-
-
?
L-phenylalanine + H2O + O2
2-oxo-3-phenylpropanoate + NH3 + H2O2
show the reaction diagram
-
about 74% activity compared to L-methionine
-
-
?
L-phenylalanine + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
inhibition of T cell proliferation through phenylalanine degradation
activity measurement by measuring H2O2 production
-
?
L-phenylglycine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
5% of the activity compared to L-alanine
-
?
L-phenylglycine + H2O + O2
phenylacetate + NH3 + H2O2
show the reaction diagram
Q8VPD4
5% of the activity with L-Ala
-
-
?
L-Pro + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
less than 10% of the activity with L-Phe
-
-
?
L-proline + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-propargylglycine + H2O + O2
2-oxopent-4-ynoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-propargylglycine + H2O + O2
3-amino-5-methylene-5H-furan-2-one + NH3 + H2O2
show the reaction diagram
-
the substrate L-propargylglycine competes with the substrate L-Phe during inactivation of the enzyme
-
-
?
L-Ser + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
72% of the activity with L-Ala
-
-
?
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
not
-
-
-
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
72% of the activity compared to L-alanine
-
?
L-serine + H2O + O2
3-hydroxy-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
about 12% activity compared to L-methionine
-
-
?
L-threonine + H2O + O2
2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-threonine + H2O + O2
2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-threonine + H2O + O2
3-hydroxy-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
about 11% activity compared to L-methionine
-
-
?
L-Trp + H2O + O2
indole-3-pyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
12% of the activity with L-Ala
-
-
?
L-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Trp + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
low reaction rate
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
high reaction rate
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Bothrops sp.
-
high reaction rate
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
12% of the activity compared to L-alanine
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
about 30% of the activity with L-Phe
-
-
?
L-tryptophan + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
good substrate
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-tryptophan, L-leucine, L-histidine, L-phenylalanine, L-arginine, L-isoleucine
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
high affinity for the substrate
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
high enzyme activity
-
-
?
L-tryptophan + H2O + O2
3-indole-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
one of the best substrates
-
-
?
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
about 63% activity compared to L-methionine
-
-
?
L-tryptophan + H2O + O2
indole-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
about 55% activity compared to L-methionine
-
-
?
L-Tyr + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
54% of the activity with L-Ala
-
-
?
L-Tyr + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
about 40% of the activity with L-Phe
-
-
?
L-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Tyr + H2O + O2
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
Q8VPD4
54% of the activity compared to L-alanine
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
high affinity for the substrate
-
-
?
L-tyrosine + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
about 40% activity compared to L-methionine
-
-
?
L-tyrosine + H2O + O2
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
about 68% activity compared to L-methionine
-
-
?
L-Val + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
B5AR80
-
-
-
?
L-Val + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
Q8VPD4
32% of the activity with L-Ala
-
-
?
L-Val + H2O + O2
3-methyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
the substrate is not effectively oxidized
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
very low activity
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
Q8VPD4
32% of the activity compared to L-alanine
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
low enzyme activity
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
less than 10% activity compared to L-methionine
-
-
?
L-valine + H2O + O2
3-methyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
about 22% activity compared to L-methionine
-
-
?
lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
Nepsilon-acetyl-L-Lys + H2O + O2
6-acetylamino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Q8VPD4
46% of the activity with L-Ala
-
-
?
Nepsilon-acetyl-L-lysine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
Q8VPD4
46% of the activity compared to L-alanine
-
?
Nepsilon-acetyl-L-lysine + H2O + O2
2-oxo-6-acetylaminohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
O-carbamoyl-L-serine + H2O + O2
3-O-carbamoyl-2-keto-propionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
O-carbamoyl-L-serine + H2O + O2
3-O-carbamoyl-2-keto-propionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O + O2
4-thioadenosyl-2-oxobutyrate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O + O2
4-thioadenosyl-2-oxobutyrate + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-homocysteine + H2O + O2
4-thioadenosyl-2-oxobutyrate + NH3 + H2O2
show the reaction diagram
Crotalus terrificus terrificus
-
-
-
-
-
S-allyl-L-cysteine + H2O + O2
2-oxo-3-(prop-2-en-1-ylsulfanyl)propanoic acid + NH3 + H2O2
show the reaction diagram
-
94% of the activity with L-methionine
-
-
?
S-allylmercapto-L-cysteine + H2O + O2
2-oxo-3-[(2-thioxoethyl)sulfanyl]propanoic acid + NH3 + H2O2
show the reaction diagram
-
26% of the activity with L-methionine
-
-
?
S-benzothiazolyl-L-cysteine + H2O + O2
3-(1,3-benzothiazol-3(2H)-ylsulfanyl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
161% of the activity with L-methionine
-
-
?
S-carbamoyl-L-cysteine + H2O + O2
3-thiocarbamoyl-2-keto-propionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
S-carbamoyl-L-cysteine + H2O + O2
3-thiocarbamoyl-2-keto-propionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
S-methylmercapto-L-cysteine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
66% of the activity with L-methionine
-
-
?
S-penta-1,3-dienylmercapto-L-cysteine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
7% of the activity with L-methionine
-
-
?
S-propylmercapto-L-cysteine + H2O + O2
2-oxo-3-[(3-thioxopropyl)sulfanyl]propanoic acid + NH3 + H2O2
show the reaction diagram
-
16% of the activity with L-methionine
-
-
?
thyroxine + H2O + O2
3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenylpyruvate] + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
triiodothyronine + H2O + O2
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
mandelic acid + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
-
additional information
?
-
Providencia rettgeri, Crotalus terrificus terrificus
-
investigation of oxidative deamination of sulfur amino acids
-
-
-
additional information
?
-
-
2 L-amino acid oxidases with different specificity: 1. oxidative deamination of aromatic carboxylic, sulfur containing imino and beta-hydroxy-L-amino acids, no activity to basic L-amino acids or L-citrulline, 2. oxidative deamination of L-arginine, L-histidine, L-ornithine, L-citrulline and L-lysine, with no affinity for any of the other L-amino acids tested
-
-
-
additional information
?
-
-
shows apoptosis-inducing activity in cell cultures
-
-
-
additional information
?
-
-
shows apoptosis-inducing activity in cell cultures
-
-
-
additional information
?
-
-
not: alpha-hydroxyisobutyrate
-
-
-
additional information
?
-
-
cytotoxic to different cell lines, resulting in loss of ability in attachment and inhibition of cell proliferation
-
-
-
additional information
?
-
snake
-
enzymatic properties of snake venom enzymes, mechanisms of enzyme-induced platelet aggregation and apoptosis
-
-
-
additional information
?
-
-
not: proline
-
-
-
additional information
?
-
-
not: proline
-
-
-
additional information
?
-
O09046
reacts in decreasing order Phe, Met,Tyr, Cys, Leu, His, other amino acids
-
-
-
additional information
?
-
-
induces human platelet aggregation
-
-
-
additional information
?
-
-
S-carbamoyl-and S-thiocarbamoyl derivatives of L-cysteine
-
-
-
additional information
?
-
snake
-
overview on assays
-
-
-
additional information
?
-
-
proline and N-methyl derivatives of leucine, methionine, homocysteine and S-benzylhomocysteine also oxidized
-
-
-
additional information
?
-
-
not: L-threonine
-
-
-
additional information
?
-
-
not: L-threonine
-
-
-
additional information
?
-
-
not: L-threonine
-
-
-
additional information
?
-
-
2 distinct L-amino acid oxidases with different specificity, 1. aliphatic or aromatic L-amino acids with non-polar side chains, 2. L-amino acids with positively charged side chains
-
-
-
additional information
?
-
-
not: D-amino acids, N,N-dimethylleucine, alpha-aminoisobutyric acid
-
-
-
additional information
?
-
-
has bactericidal activity
-
-
-
additional information
?
-
-
induces apoptosis
-
-
-
additional information
?
-
-
induction of apoptosis
-
-
-
additional information
?
-
-
the enzyme has anticoagulant activity that impairs the intrinsic clotting by inhibiting factor IX
-
-
-
additional information
?
-
-
the enzyme induces platelet aggregation and shows bactericidal activity against Escherichia coli and Staphylococcus aureus. The enzyme is slightly hemorrhagic and induces edema in mouse
-
-
-
additional information
?
-
-
enzyme possesses anticoagulant activity
-
-
-
additional information
?
-
-
formation of H2O2 is essential for the enzyme to induce platelet aggregation
-
-
-
additional information
?
-
-
induces platelet aggregation
-
-
-
additional information
?
-
-
inhibits aggregation of platelets
-
-
-
additional information
?
-
-
inhibits platelet aggregation
-
-
-
additional information
?
-
-
the enzyme has a preference for aromatic amin acids, less than 5% of the activity with L-Phe: Gln, Gly, Asn, His, Leu, Glu, Lys
-
-
-
additional information
?
-
-
enzyme induces dose-dependent platelet aggregation, which is abolished by catalase, inhibits the growth of Gram-negative and Gram-positive bacteria and has antileishmanic activity
-
-
-
additional information
?
-
-
enzyme induces necrosis and apoptosis in Jurkat cells, in presence of catalase that scavenges H2O2 a switch to apoptosis is observed
-
-
-
additional information
?
-
-
enzyme induces time-dependent platelet aggregation, mouse paw edema, shows cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells
-
-
-
additional information
?
-
A1IGW6, -
enzyme shows potent antibacterial activity against fish pathogens
-
-
-
additional information
?
-
-, Q6T277
enzyme shows tumoricidal activity
-
-
-
additional information
?
-
-, Q6IWZ0
native enzyme inhibits the growth of Gram-positive and Gram-negative bacteria, including marine bacteria, and also inhibits the growth of yeasts and fungi. In minimal media, enzyme is bacteriostatic and not bactericidal, but becomes bactericidal in media enriched with Tryptone Peptone
-
-
-
additional information
?
-
A1IGW6, -
enzyme uses only L-lysine as substrate and is ineffective with other proteinaceous amino acids or with D-lysine
-
-
-
additional information
?
-
-, Q6IWZ0
little or no activity with amino acids other than L-Arg, L-Lys
-
-
-
additional information
?
-
-, Q6T277
no substrate: D-lysine
-
-
-
additional information
?
-
-
active site and substrate binding structure, overview
-
-
-
additional information
?
-
-
does not catalyze N-acetyL-L-cysteine and cis-4-hydroxyl-L-proline oxidation
-
-
-
additional information
?
-
-
does not hydrolyze casein and synthetic substrates including N-benzoyl-L-arginine ethylester or N-benzoyl-L-arginine p-nitroanilide
-
-
-
additional information
?
-
Synechococcus elongatus PCC 7942, Synechococcus cedrorum PCC 6908
-
high specificity for basic L-amino acids
-
-
-
additional information
?
-
P72346
high specificity for basic L-amino acids
-
-
-
additional information
?
-
-
L-histidine, L-glutaminic acid, L-tyrosine, L-isoleucine, L-glutamine and L-lysine are poor substrates
-
-
-
additional information
?
-
Q6TGQ9
L-serine, L-proline, L-glycine, L-threonine and L-cysteine are not oxidized
-
-
-
additional information
?
-
-
no cleavage of D-phenylalanine and L-aspartic acid
-
-
-
additional information
?
-
A1IGW6, -
SSAP catalyzes oxidation of only L-lysine and is ineffective with any other proteinaceous amino acids. No LAO activity is detected when L-lysine is replaced with D-lysine
-
-
-
additional information
?
-
-, Q6IWZ0
bactericidal activity of the sea hare secretion against Escherichia coli, Vibrio harveyi, Staphylococcus aureus, and Pseudomonas aeruginosa
-
-
-
additional information
?
-
Q4F867
LAAO dose-dependently inhibits platelet aggregation and exhibits antibactericidal activity
-
-
-
additional information
?
-
-
LAAO exhibits a cytotoxic effect on A549 cells and causes up to 41.2% apoptosis of A549 cells, overview
-
-
-
additional information
?
-
B5AR80
LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase
-
-
-
additional information
?
-
-
mouse milk contains L-amino acid oxidase, a lactating mammary gland-specific protein, that displays antibacterial activity in vitro through the production of hydrogen peroxide from free amino acids
-
-
-
additional information
?
-
B5U982
MPLAO3 exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, and is most active against Aeromonas salmonicida strain JCM7874, which is attributable to H2O2, because the activity is completely lost in the presence of catalase
-
-
-
additional information
?
-
-, Q90W54
the LAO-containing fraction inhibits coagulation factor IX, and it prolongs the APTT, PT and fibrinogen clotting time and cleaves the Aalpha-chain of fibrinogen probably due to the presence of traces of a metalloproteinase in the fraction, overview. The mechanism for the anticoagulant activity of M-LAO fraction is not due to binding to factor IX. The fibrinogenolytic activity of the M-LAO fraction is independent of either LAO enzymatic activity or generated hydrogen peroxide
-
-
-
additional information
?
-
-
the venom enzyme from Bothrops jararaca show high antibacterial activity, e.g. against Staphylococcus aureus, via the produced H2O2, overview. The enzyme also shows hemolytic and leishmanicidal activity, overview
-
-
-
additional information
?
-
-, Q6IWZ0
effect of pH on conversion of escapin intermediates to escapin end products, overview
-
-
-
additional information
?
-
-, Q90W54
L-amino acid oxidase is a dimeric glycosylated flavoenzyme that catalyses the oxidative deamination of an L-amino acid substrate to a 2-oxo acid along with the production of ammonia and hydrogen peroxide
-
-
-
additional information
?
-
-
LAAO is very active against L-Tyr, L-Asp, L-Phe, L-Glu, L-Trp, L-His, L-Gln, L-Ile, L-Met, L-Leu and moderately active against L-Lys, L-Arg, L-Ala and L-Asn, overview
-
-
-
additional information
?
-
B5AR80
substrate specificity, no activity with L-Pro, L-Thr, L-Ser, and L-Cys, overview. Construction of a LAAO structural model of the stereochemistry of substrate binding by the enzyme, overview
-
-
-
additional information
?
-
Q4F867
substrate specificity, overview. No activity with L-Pro, L-Asn, Gly, L-Ser, and L-Cys. The enzyme shows no hemorrhagic activity
-
-
-
additional information
?
-
-
Bothrops jararaca venom induces programmed cell death in epimastigotes of Trypanosoma cruzi due to its L-AAO activity, L-AAO activity induces parasites cytoplasmic retraction, mitochondrial swelling and DNA fragmentation, overview. L-AAO produces H2O2, which is highly toxic
-
-
-
additional information
?
-
-
substrate specificity, overview. L-Thr is a poor substrate
-
-
-
additional information
?
-
-
the antibacterial protein is an L-amino acid oxidase with broad substrate specificity
-
-
-
additional information
?
-
D2KWB6
the enzyme shows antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus, overview
-
-
-
additional information
?
-
-
the L-amino acid oxidase from Bothrops marajoensis is able to inhibit the growth of several microorganisms, including Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa,and Leishmania sp.. The enzyme inhibits the growth of promastigote forms of Leishmania amazonensis and Leishmania chagasi with IC50 values of 2.55 mg/mL and 2.86 mg/mL, respectively
-
-
-
additional information
?
-
-
toxophallin induces chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis, effect towards human leukemia CEM-T4 and murine leukemia L1210 cells, overview, L-cysteine, L-glycine, L-proline, L-oxyproline, DL-serine, and DL-aspartic acid are no substrates. Toxophallin is also not active against benzylamine, ethanolamine, diethylamine, meta- and para-phenylendiamine, ortho-, meta- and paraaminophenols, or putrescin
-
-
-
additional information
?
-
-
LAAO catalyzes the stereospecific oxidative deamination of an L-amino acid substrate, releasing the corresponding 2-oxo acid and resulting in the production of hydrogen peroxide and ammonia
-
-
-
additional information
?
-
-
the enzyme from Rhodococcus sp. AIU Z-35-1 shows broad substrate specificity
-
-
-
additional information
?
-
-
glycine is not oxidized by the enzyme
-
-
-
additional information
?
-
-
no activity with L-lysine, L-cysteine, L-serine, L-threonine, and L-cystine
-
-
-
additional information
?
-
-
the enzyme does not oxidize other L-amino acids and D-amino acids besides L-lysine, L-ornithine and L-arginine
-
-
-
additional information
?
-
Laccaria bicolor S238N
-
no cleavage of D-phenylalanine and L-aspartic acid
-
-
-
additional information
?
-
Corynebacterium sp. A20
-
not: proline, not: L-threonine
-
-
-
additional information
?
-
Pseudoalteromonas flavipulchra C2
-
the antibacterial protein is an L-amino acid oxidase with broad substrate specificity
-
-
-
additional information
?
-
Aplysia californica Cooper 1863
Q6IWZ0
effect of pH on conversion of escapin intermediates to escapin end products, overview
-
-
-
additional information
?
-
Rhodococcus sp. AIU Z-35-1
-
the enzyme from Rhodococcus sp. AIU Z-35-1 shows broad substrate specificity
-
-
-
additional information
?
-
Hebeloma cylindrosporum CBS 558.96
-
no cleavage of D-phenylalanine and L-aspartic acid
-
-
-
additional information
?
-
-
the enzyme does not oxidize other L-amino acids and D-amino acids besides L-lysine, L-ornithine and L-arginine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
an L-amino acid + H2O + O2
a 2-oxo-acid + NH3 + H2O2
show the reaction diagram
-
L-amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective imino acids with concomitant hydrolysis to alpha-keto acids, together with the production of ammonia and hydrogen peroxide
-
-
?
DL-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
DL-leucine + H2O + O2
2-oxo-4-methylpentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
DL-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
DL-norleucine + H2O + O2
2-oxocaproate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
DL-threonine + H2O + O2
2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
DL-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
DL-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
first step in reaction sequence of formation of phenylacetic acid from L-phenylalanine
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
functional in photosynthetic and respiratory activities as well as in L-arginine degradation
-
-
-
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
functional in photosynthetic and respiratory activities as well as in L-arginine degradation
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
utilization of amino acids as nitrogen source
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
operates as scavanger of ammonium from extracellular amino acids
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
bactericidal action of L-amino acid oxidase
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
involved in the water-splitting reaction of photosystem II
-
-
-
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
involved in the water-splitting reaction of photosystem II
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
O09046
responsible for killing bacteria in the mammary gland
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
snake
-
biological functions of snake venom enzymes
-
-
-
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
cell death induced by the enzyme is of two types, one non-apoptotic and the other apoptotic. The effect is not dependent on the acute toxicity of H2O2
-
-
?
L-amino acid + H2O + O2
2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
Corynebacterium sp. A20
-
utilization of amino acids as nitrogen source
-
-
?
L-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-arginine + H2O + O2
2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-glutamine + H2O + O2
2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-histidine + H2O + O2
2-oxo-4-imidazolepropionic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylpentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-leucine + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-, Q6IWZ0
the ink of sea hares contains escapin, an L-amino acid oxidase that metabolizes L-lysine, thereby producing a mixture that kills microbes and deters attacking predators. This secretion contains H2O2, ammonia, and an equilibrium mixture of socalled escapin intermediate product, EIP-K, that includes 6-amino-2-oxohexanoic acid and several other molecules. Components of the equilibrium mixture react nonenzymatically with H2O2 to form escapin end product, i.e. EEP-K, which contains 2-aminovaleric acid and 2-valerolactam. The proportions of the components in this equilibrium mixture change with pH, and this is biologically important because the secretion has pH 5 when released but becomes pH 8 when fully diluted in seawater, overview
-
-
?
L-Lys + H2O + O2
6-amino-2-oxo-hexanoic acid + NH3 + H2O2
show the reaction diagram
B5U982
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Aplysia californica Cooper 1863
Q6IWZ0
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
strictly specific for
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Rhodococcus sp. AIU Z-35-1
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
preferred substrate
-
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
primary amino acid catabolized by IL4I1
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Pseudoalteromonas flavipulchra C2
-
-
-
-
?
L-phenylalanine + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
inhibition of T cell proliferation through phenylalanine degradation
activity measurement by measuring H2O2 production
-
?
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-tyrosine + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
-
induces apoptosis
-
-
-
additional information
?
-
-
induction of apoptosis
-
-
-
additional information
?
-
-
the enzyme has anticoagulant activity that impairs the intrinsic clotting by inhibiting factor IX
-
-
-
additional information
?
-
-
the enzyme induces platelet aggregation and shows bactericidal activity against Escherichia coli and Staphylococcus aureus. The enzyme is slightly hemorrhagic and induces edema in mouse
-
-
-
additional information
?
-
-
enzyme induces dose-dependent platelet aggregation, which is abolished by catalase, inhibits the growth of Gram-negative and Gram-positive bacteria and has antileishmanic activity
-
-
-
additional information
?
-
-
enzyme induces necrosis and apoptosis in Jurkat cells, in presence of catalase that scavenges H2O2 a switch to apoptosis is observed
-
-
-
additional information
?
-
-
enzyme induces time-dependent platelet aggregation, mouse paw edema, shows cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells
-
-
-
additional information
?
-
A1IGW6, -
enzyme shows potent antibacterial activity against fish pathogens
-
-
-
additional information
?
-
-, Q6T277
enzyme shows tumoricidal activity
-
-
-
additional information
?
-
-, Q6IWZ0
native enzyme inhibits the growth of Gram-positive and Gram-negative bacteria, including marine bacteria, and also inhibits the growth of yeasts and fungi. In minimal media, enzyme is bacteriostatic and not bactericidal, but becomes bactericidal in media enriched with Tryptone Peptone
-
-
-
additional information
?
-
-, Q6IWZ0
bactericidal activity of the sea hare secretion against Escherichia coli, Vibrio harveyi, Staphylococcus aureus, and Pseudomonas aeruginosa
-
-
-
additional information
?
-
Q4F867
LAAO dose-dependently inhibits platelet aggregation and exhibits antibactericidal activity
-
-
-
additional information
?
-
-
LAAO exhibits a cytotoxic effect on A549 cells and causes up to 41.2% apoptosis of A549 cells, overview
-
-
-
additional information
?
-
B5AR80
LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase
-
-
-
additional information
?
-
-
mouse milk contains L-amino acid oxidase, a lactating mammary gland-specific protein, that displays antibacterial activity in vitro through the production of hydrogen peroxide from free amino acids
-
-
-
additional information
?
-
B5U982
MPLAO3 exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, and is most active against Aeromonas salmonicida strain JCM7874, which is attributable to H2O2, because the activity is completely lost in the presence of catalase
-
-
-
additional information
?
-
-, Q90W54
the LAO-containing fraction inhibits coagulation factor IX, and it prolongs the APTT, PT and fibrinogen clotting time and cleaves the Aalpha-chain of fibrinogen probably due to the presence of traces of a metalloproteinase in the fraction, overview. The mechanism for the anticoagulant activity of M-LAO fraction is not due to binding to factor IX. The fibrinogenolytic activity of the M-LAO fraction is independent of either LAO enzymatic activity or generated hydrogen peroxide
-
-
-
additional information
?
-
-
the venom enzyme from Bothrops jararaca show high antibacterial activity, e.g. against Staphylococcus aureus, via the produced H2O2, overview. The enzyme also shows hemolytic and leishmanicidal activity, overview
-
-
-
additional information
?
-
-
Bothrops jararaca venom induces programmed cell death in epimastigotes of Trypanosoma cruzi due to its L-AAO activity, L-AAO activity induces parasites cytoplasmic retraction, mitochondrial swelling and DNA fragmentation, overview. L-AAO produces H2O2, which is highly toxic
-
-
-
additional information
?
-
-
substrate specificity, overview. L-Thr is a poor substrate
-
-
-
additional information
?
-
-
the antibacterial protein is an L-amino acid oxidase with broad substrate specificity
-
-
-
additional information
?
-
D2KWB6
the enzyme shows antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus, overview
-
-
-
additional information
?
-
-
the L-amino acid oxidase from Bothrops marajoensis is able to inhibit the growth of several microorganisms, including Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa,and Leishmania sp.. The enzyme inhibits the growth of promastigote forms of Leishmania amazonensis and Leishmania chagasi with IC50 values of 2.55 mg/mL and 2.86 mg/mL, respectively
-
-
-
additional information
?
-
-
toxophallin induces chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis, effect towards human leukemia CEM-T4 and murine leukemia L1210 cells, overview
-
-
-
additional information
?
-
Pseudoalteromonas flavipulchra C2
-
the antibacterial protein is an L-amino acid oxidase with broad substrate specificity
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FAD
-
2 mol of FAD per mol of enzyme
FAD
-
2 mol of FAD per mol of enzyme
FAD
-
2 mol of FAD per mol of enzyme
FAD
-
4 mol of FAD per mol of enzyme
FAD
-
1 mol of FAD per mol of enzyme
FAD
-
non-covalently bound
FAD
Bothrops sp.
-
-
FAD
Bacillus carotarum
-
2 mol of FAD per mol of enzyme
FAD
Q8VPD4
a homodimer complex containing 2 FAD molecules
FAD
-
non-covalently bound
FAD
-
; the whole cofactor is buried inside the protein and is not solvent accessible. The FAD-binding domain consists of three discontinuous regions of the structure: residues 4-51, 239-314 and 423-488. The main structural feature of this domain is a fivestranded beta-pleated sheet sandwiched between three alpha-helices and a four-stranded beta-pleated sheet. The FAD-binding domain corresponds to a general topology throughout the whole GR2-famil
FAD
Synechococcus cedrorum PCC 6908
-
-
FAD
-
non-covalently bound
FAD
B5AR80
a flavoenzyme
FAD
-
the FAD-binding domain includes residues 35-72, 239-318 and 446-486. The secondary structure elements of this domain are 4 alpha-helices
flavin
-, Q90W54
LAO is a flavoenzyme
flavin
-
a flavoenzyme
FMN
-
2 mol of FMN per mol of enzyme
FMN
-
FMN firmly bound to enzyme
FMN
-
6 mol of FMN per mol of enzyme
FMN
-
2 mol of FMN per mol of enzyme
FMN
-
contains 2 mol of FMN per mol of enzyme
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activates
Ce3+
-
activates
Cl-
-
favors reaction
Co2+
-
activates
Mg2+
-
activates
Mn2+
-
MnCl2 inhibits
Mn2+
-
powerful activator
Nd3+
-
activates
Tb3+
-
activates
Zn2+
-
Akbu-LAAO contains four Zn2 per dimer, that are unessential for the hydrolytic activity of the enzyme. The apoenzyme shows 61% of maximal activity. The zinc ions probably help maintain the structural integrity of the enzyme
Zn2+
-
one Zn2+ ion is anchored to His75 at the beta-hairpin formed by the segment 73-86, which stabilizes the oligomeric state by bridging His75 and Glu279 of the adjacent protomer
Zn2+
-
required
Zn2+
-
the zinc ion plays a role in tetramer stabilization, but not in enzyme activity
Mn2+
-
activates
additional information
-
magnesium, iron and molybdene are no activators
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-aminobenzoic acid
-
three and two binding sites in isoforms L1 and L2, respectively
8-hydroxyquinoline
-
76% residual activity at 1 mM (pH 5.5)
anthranilate
-
competitive
aromatic carboxylates
-
competitive
bathophenanthroline disulfonic acid
-
-
Benzenearsonic acid
-
-
Benzoate
-
and derivatives
Butanedione
snake
-
good substrates but not poor substrates protect against inactivation
Ca2+
-
inhibition can be relieved by L-arginine
CaCl2
Synechococcus cedrorum PCC 6908
-
-
Chloropromazine
-
inhibition can be relieved by L-arginine
Co2+
-
67% residual activity at 1 mM (pH 5.5)
Cu2+
-
in presence of the activator Mn2+
EDTA
-
10 mM, 90% inhibition
EDTA
-
inhibits platelet aggregation induced by LAAO
EDTA
-
80% residual activity at 1 mM (pH 5.5)
EMD 132338
-
inhibits platelet aggregation induced by LAAO
Fe2+
-
62% residual activity at 1 mM (pH 5.5)
-
Gd3+
-
inhibits 17% at 10 mM
Gly
Q8VPD4
competitive inhibition of reaction with L-Ala, does not reduce activity when 10 mM L-Phe is used as substrate
glycine
Q8VPD4
activity strongly decreased in glycine/NaOH-buffer, competitive inhibition, no inhibition, when 10 mM L-phenylanalnine is used as substrate
Hg2+
-
complete inhibition at 1 mM (pH 7.0)
hydrazine
-
46% residual activity at 1 mM (pH 5.5)
hydroxylamine
-
5 mM, 75% inhibition
hydroxylamine
-
63% residual activity at 1 mM (pH 5.5)
iodoacetic acid
-
0.5% residual activity at 1 mM (pH 7.0)
KCN
-
10 mM, complete inhibition
KCN
-
70% residual activity at 1 mM (pH 7.0)
L-alanine
Q8VPD4
-
L-arginine
Q8VPD4
; pH 7.6, 30C
L-citrulline
Q8VPD4
; pH 7.6, 30C
L-leucine
Q8VPD4
; pH 7.6, 30C
L-lysine
Q8VPD4
; pH 7.6, 30C
L-methionine
Q8VPD4
; pH 7.6, 30C
L-Norleucine
Q8VPD4
; pH 7.6, 30C
L-ornithine
Q8VPD4
pH 7.6, 30C
L-phenylalanine
Q8VPD4
; pH 7.6, 30C
L-Propargylglycine
-
irreversible inhibition in a dose- and time-dependent manner
L-serine
Q8VPD4
pH 7.6, 30C
L-tyrosine
Q8VPD4
; pH 7.6, 30C
m-Aminobenzoate
-
-
m-chlorobenzoate
-
-
m-chlorobenzoate
-, Q90W54
LAO inhibitor, does not inhibit the degradation of fibrinogen
m-fluorobenzoate
-
-
m-Hydroxybenzoate
-
-
m-Nitrobenzoate
-
-
Mg2+
-
EDTA, ATP and ADP, but not AMP can overcome inhibition
Mg2+
-
78% residual activity at 1 mM (pH 5.5)
Mn2+
-
63% residual activity at 1 mM (pH 5.5)
N-acetyl-L-tryptophan amide
-
inhibition of both isoforms L1 and L2
N-acetyltryptophan
-
inhibition of both isoforms L1 and L2
Na+
-
sodium salts, order of effectiveness: SCN-, NO3-, Cl-, Br-, I-, F-, HCOO-, CH3COO-
NaF
-
10 mM, 85% inhibition
NH4+
-
inhibition of amino acid oxidase activity, no inhibition of L-hydroxy acid oxidase activity
Ni2+
-
55% residual activity at 1 mM (pH 5.5)
nordihydroguaiaretic acid
-
-
o-aminobenzoate
-
-
o-aminobenzoic acid
-, Q90W54
LAO inhibitor, slightly inhibiting the degradation of fibrinogen
o-chlorobenzoate
-
-
o-fluorobenzoate
-
-
o-hydroxybenzoate
-
-
o-mercaptobenzoate
-
-
o-Nitrobenzoate
-
-
o-phenanthroline
-
15% residual activity at 1 mM (pH 5.5)
orthanilic acid
-
-
p-Aminobenzoate
-
-
p-Aminobenzoic acid
-
-
p-Chlorobenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-fluorobenzoate
-
-
p-hydroxybenzoate
-
-
p-nitrobenzoate
-
-
phenylhydrazine
-
complete inhibition at 1 mM (pH 5.5)
Semicarbazide
-
32% residual activity at 1 mM (pH 5.5)
Sodium azide
-
slight
Thiosemicarbazide
-
5 mM, 40% inhibition
Tunicamycin
-
inhibits secretion of recombinant LAAO
Zn2+
-
24% residual activity at 1 mM (pH 5.5)
additional information
-
good substrates, not poor substrates are inhibitors; substrate inhibition
-
additional information
-
substrate inhibition
-
additional information
-
substrate inhibition
-
additional information
-
insensitive to KCN at 1 mM; substrate inhibition
-
additional information
-
substrate inhibition
-
additional information
-
naturally occuring inhibitors
-
additional information
-
inhibition by cations increases in alkaline pH, inhibition by anions increases in acidic pH
-
additional information
snake
-
not: 5,5-dithiobis-(2-nitrobenzoic acid), trinitrobenzene sulfonic acid
-
additional information
A1IGW6
catalase almost completely abolishs the antibacterial activity of SSAP, indicating that H2O2 is the mediator of the activity of SSAP
-
additional information
-
LAAO has no activity on platelets in platelet-rich plasma. Catalase inhibits the platelet aggregation and platelet protein phosphorylation induced by LAAO
-
additional information
-
antimicrobial activity can be partially inhibited by catalase
-
additional information
Q6TGQ9
catalase inhibits dose-dependent parasite killing
-
additional information
Q6TGQ8
catalase inhibits dose-dependent parasite killing
-
additional information
-
antiparasite effects are inhibited by catalase
-
additional information
-
antibacterial activity of SSAP is completely abolished by addition of catalase
-
additional information
-
the antibacterial and anti-aggregating activity of LAAO is abolished by catalase
-
additional information
-
initial uncompetitive inhibition of L1 followed by mixed inhibition at higher concentrations suggesting the existence of two different inhibitor-binding sites distinct from the substrate-binding site. In the case of L2, initial linear competitive inhibition followed by mixed inhibition suggesting the existence of two nonoverlapping inhibitor-binding sites, one of which is the substrate-binding site
-
additional information
-
antibacterial activities are completely abolished by catalase
-
additional information
-
is inactivated by freezing or low pH. Bactericidal, antitumoral, trypanocidal, edematogenic, platelet-aggregating activities, and apoptotic DNA fragmentation in HL-60 cells are inhibited by catalase
-
additional information
-
catalase (1.5 micromol) at various concentrations of leucine does not completely inhibit cell death caused by LAAO (1.2 micromol) in leucine auxotrophic strain
-
additional information
-
no inhibition by caspase III inhibitor benzyloxycarbonyl-Asp(OMe)-fluoromethylketone
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
alpha,alpha'-dipyridyl
-
119% activity at 1 mM (pH 7.0)
CaCl2
-
2 mM, 50% activation
N-ethylmaleimide
-
remarkably enhances the lysine oxidase activity (500% activity at 1 mM (pH 7.0))
p-chloromercuribenzoic acid
-
remarkably enhances the lysine oxidase activity
Sodium azide
-
116% activity at 1 mM (pH 7.0)
KCN
-
enhances the lysine oxidase activity (177% activity at 1 mM (pH 5.5))
additional information
-
antibacterial activity of SSAP results from the generation of H2O2
-
additional information
-
the glycosylation site of the enzyme is not important for structural delineation of the active site
-
additional information
-
peptone up-regulates expression of the aaoSo gene
-
additional information
Hebeloma sp.
-
activities are the highest in mycelia grown in nitrogen-rich conditions
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.05
-
cysteine
-
-
3.1
-
djenkolic acid
Crotalus terrificus terrificus
-
-
83
-
djenkolic acid
-
-
0.274
-
L-Ala
Q8VPD4
pH 7.6, 30C
28.3
-
L-Ala
-
pH 8.5, 25C
116.5
-
L-Ala
-
pH 7.5, 0.9% NaCl, at 25C
0.274
-
L-alanine
Q8VPD4
pH 7.6, 30C
0.31
-
L-alanine
Q8VPD4
wild-type enzyme, 30C, pH 7.6
0.45
-
L-alanine
Q8VPD4
recombinant enzyme, expressed in Streptomyces lividans, 30C, pH 7.6
5.12
-
L-alanine
-
-
12.5
-
L-alanine
-
-
5
-
L-aminobutyric acid
-
-
0.07
-
L-Arg
Q8VPD4
pH 7.6, 30C
12.2
-
L-Arg
-
pH 7.5, 0.9% NaCl, at 25C
18.75
-
L-Arg
-
pH 8.5, 25C
0.037
-
L-arginine
Bacillus carotarum
-
-
0.07
-
L-arginine
Q8VPD4
pH 7.6, 30C
0.27
-
L-arginine
-
fraction II
2.33
-
L-arginine
-
-
2.9
-
L-arginine
-
-
4
-
L-arginine
Synechococcus cedrorum PCC 6908
-
-
19.07
-
L-Asn
-
pH 8.5, 25C
0.028
-
L-Asp
Q8VPD4
pH 7.6, 30C
0.04
-
L-Asp
-
pH 8.5, 25C
0.028
-
L-asparagine
Q8VPD4
pH 7.6, 30C
0.259
-
L-asparagine
Bacillus carotarum
-
-
0.36
-
L-asparagine
-
-
2.5
-
L-asparagine
-
-
0.026
-
L-citrulline
Q8VPD4
pH 7.6, 30C
21.3
-
L-citrulline
-
fraction II
4.5
-
L-cysteine
Crotalus terrificus terrificus
-
-
5.3
-
L-cysteine
-
-
5
-
L-cystine
Crotalus terrificus terrificus
-
-
0.085
-
L-Gln
Q8VPD4
pH 7.6, 30C
5.88
-
L-Gln
-
pH 8.5, 25C
0.411
-
L-Glu
Q8VPD4
pH 7.6, 30C
1.44
-
L-Glu
-
pH 8.5, 25C
0.411
-
L-Glutamic acid
Q8VPD4
pH 7.6, 30C
0.085
-
L-glutamine
Q8VPD4
pH 7.6, 30C
0.34
-
L-glutamine
Bacillus carotarum
-
-
4.84
-
L-His
-
pH 8.5, 25C
1.8
-
L-histidine
-
fraction II
2.3
-
L-histidine
A8QL50, -
-
2.5
-
L-histidine
-
-
4.97
-
L-histidine
-
-
6.2
-
L-histidine
-
-
1.3
-
L-homocysteine
Crotalus terrificus terrificus, Providencia rettgeri
-
-
0.72
-
L-homocystine
Crotalus terrificus terrificus
-
-
12.5
-
L-homocystine
-
-
1.4
-
L-Ile
-
pH 7.5, 0.9% NaCl, at 25C
1.64
-
L-Ile
-
pH 8.5, 25C
5.11
-
L-Ile
Q8VPD4
pH 7.6, 30C
0.00144
-
L-isoleucine
-
isoform L1
0.00189
-
L-isoleucine
-
isoform L2
0.8
-
L-isoleucine
-
-
2.9
-
L-isoleucine
A8QL50, -
-
4.02
-
L-isoleucine
-
-
5.11
-
L-isoleucine
Q8VPD4
pH 7.6, 30C
71
-
L-isoleucine
-
fraction I
0.028
-
L-Leu
Q8VPD4
pH 7.6, 30C
0.25
-
L-Leu
-
-
0.49
-
L-Leu
-
pH 7.5, 0.9% NaCl, at 25C
2.1
-
L-Leu
-
pH 4.7, 37C
60.7
-
L-Leu
-
pH 8.5, 25C
0.00923
-
L-leucine
-
in 50 mM Tris-HCl buffer pH 8.0, at 37C
0.028
-
L-leucine
Q8VPD4
pH 7.6, 30C
0.0465
-
L-leucine
-
pH 8.0, 37C
0.11
-
L-leucine
-
-
0.14
-
L-leucine
-
-
0.4
-
L-leucine
-
pH 8.5, 25C
0.56
-
L-leucine
-
-
0.6
-
L-leucine
-
isoform L2
0.63
-
L-leucine
-
-
0.75
-
L-leucine
-
-
0.75
-
L-leucine
-
isoform L1
0.97
-
L-leucine
-
at pH 8.0, temperature not specified in the publication
1
-
L-leucine
-
-
2.2
-
L-leucine
A8QL50, -
-
2.6
-
L-leucine
-
fraction I
0.015
-
L-Lys
Q8VPD4
pH 7.6, 30C
0.16
-
L-Lys
B5U982
37C, isozymes MPLAO1 and MPLAO3
21.5
-
L-Lys
-
pH 8.5, 25C
64
-
L-Lys
-
pH 7.5, 0.9% NaCl, at 25C
0.015
-
L-lysine
Q8VPD4
pH 7.6, 30C
0.04
-
L-lysine
-
-
0.19
-
L-lysine
A1IGW6
; 37C
0.37
-
L-lysine
-
pH 4.0, 37C
0.97
-
L-lysine
-
-
1.6
-
L-lysine
-
-
2.6
-
L-lysine
A8QL50, -
-
23.2
-
L-lysine
-
fraction II
0.039
-
L-Met
Q8VPD4
pH 7.6, 30C
0.373
-
L-Met
-
pH 7.5, 0.9% NaCl, at 25C
15
-
L-Met
-
pH 8.5, 25C
0.039
-
L-methionine
Q8VPD4
pH 7.6, 30C
0.223
-
L-methionine
-
isoform L2
0.24
-
L-methionine
-
-
0.297
-
L-methionine
-
isoform L1
0.35
-
L-methionine
Crotalus terrificus terrificus
-
-
0.65
-
L-methionine
-
pH 8.5, 25C
0.7
-
L-methionine
A8QL50, -
-
0.88
-
L-methionine
-
-
0.885
-
L-methionine
-
-
9.6
-
L-methionine
-
-
10
-
L-methionine
-
-
0.026
-
L-Norleucine
Q8VPD4
pH 7.6, 30C
0.13
-
L-Norleucine
-
-
0.91
-
L-norvaline
-
-
0.034
-
L-Orn
Q8VPD4
pH 7.6, 30C
0.034
-
L-ornithine
Q8VPD4
pH 7.6, 30C
6.2
-
L-ornithine
-
-
13.3
-
L-ornithine
-
-
14.2
-
L-ornithine
-
fraction II
0.022
-
L-Phe
Q8VPD4
pH 7.6, 30C
0.13
-
L-Phe
-
pH 8.5, 25C
0.142
-
L-Phe
-
pH 7.5, 0.9% NaCl, at 25C
6.5
-
L-Phe
-
-
0.011
-
L-phenylalanine
-
-
0.022
-
L-phenylalanine
Q8VPD4
pH 7.6, 30C
0.03601
-
L-phenylalanine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
0.03782
-
L-phenylalanine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
0.0493
-
L-phenylalanine
-
isoform L2
0.05
-
L-phenylalanine
-
-
0.051
-
L-phenylalanine
-
-
0.0665
-
L-phenylalanine
-
isoform L1
0.16
-
L-phenylalanine
-
-
2.2
-
L-phenylalanine
Hebeloma sp.
-
strain SE5
3.1
-
L-phenylalanine
-
fraction I
3.5
-
L-phenylalanine
-
-
4.28
-
L-phenylalanine
-
-
5
-
L-phenylalanine
Hebeloma sp.
-
strain F-NB01
11.73
-
L-phenylalanine
-
at pH 7.0 and 40C
12.01
-
L-phenylalanine
-
at pH 7.0 and 30C
12.81
-
L-phenylalanine
-
at pH 7.0 and 20C
13.52
-
L-phenylalanine
-
at pH 7.0 and 50C
7.95
-
L-Propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
11.78
-
L-Propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
1.36
-
L-Ser
Q8VPD4
pH 7.6, 30C
1.36
-
L-serine
Q8VPD4
pH 7.6, 30C
0.27
-
L-Trp
-
pH 8.5, 25C
0.318
-
L-Trp
-
pH 7.5, 0.9% NaCl, at 25C
0.08
-
L-tryptophan
-
-
0.147
-
L-tryptophan
-
-
0.17
-
L-tryptophan
-
pH 8.5, 25C
0.211
-
L-tryptophan
-
isoform L1
0.235
-
L-tryptophan
-
isoform L2
2.2
-
L-tryptophan
-
-
2.3
-
L-tryptophan
-
-
4.2
-
L-tryptophan
-
fraction I
0.019
-
L-Tyr
Q8VPD4
pH 7.6, 30C
0.05
-
L-Tyr
-
pH 8.5, 25C
0.081
-
L-Tyr
-
pH 7.5, 0.9% NaCl, at 25C
0.019
-
L-tyrosine
Q8VPD4
pH 7.6, 30C
0.052
-
L-tyrosine
-
isoform L1
0.538
-
L-tyrosine
-
isoform L2
1
-
L-tyrosine
-
fraction I
1.57
-
L-tyrosine
-
-
2.2
-
L-tyrosine
-
-
3.73
-
L-Val
Q8VPD4
pH 7.6, 30C
13.92
-
L-Val
-
pH 7.5, 0.9% NaCl, at 25C
0.27
-
L-valine
-
-
3.73
-
L-valine
Q8VPD4
pH 7.6, 30C
1.9
-
S-adenosyl-L-homocysteine
Crotalus terrificus terrificus
-
-
24.8
-
S-adenosyl-L-homocysteine
-
-
100
-
S-adenosyl-L-homocysteine sulfoxide
-
-
14
-
S-adenosylhomocysteine
-
-
14
-
S-adenosylmethionine
-
-
2.2
-
S-ribosyl-L-homocysteine
Crotalus terrificus terrificus
-
-
2.2
-
S-ribosyl-L-homocysteine
-
-
13
-
S-ribosyl-L-homocysteine
-
-
45
-
methionine sulfoxide
-
-
additional information
-
additional information
-
effect of pH on Km
-
additional information
-
additional information
-, Q6IWZ0
kinetics
-
additional information
-
additional information
-
a chiral ligand exchange CE essay with zinc(II)-L-valine complex for determining enzyme kinetic constant of LAAO, method, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
293
-
L-Ala
-
pH 8.5, 25C
0.052
2.1
L-alanine
Q8VPD4
wild-type enzyme
0.057
0.65
L-alanine
Q8VPD4
recombinant enzyme, expressed in and Streptomyces lividans, 30C, pH 7.6
26.89
-
L-alanine
-
-
228.8
-
L-Arg
-
pH 8.5, 25C
22.54
-
L-arginine
-
-
84.08
-
L-Asn
-
pH 8.5, 25C
47.49
-
L-Asp
-
pH 8.5, 25C
5.26
-
L-asparagine
-
-
430.4
-
L-Gln
-
pH 8.5, 25C
790.5
-
L-Glu
-
pH 8.5, 25C
483.1
-
L-His
-
pH 8.5, 25C
4.15
-
L-histidine
-
-
66.7
-
L-Ile
-
pH 8.5, 25C
6.33
-
L-isoleucine
-
-
26
-
L-lactic acid
-
-
31.1
-
L-Leu
-
-
1025
-
L-Leu
-
pH 8.5, 25C
1.8
-
L-leucine
-
in 50 mM Tris-HCl buffer pH 8.0, at 37C
6.3
-
L-leucine
-
-
47.98
-
L-leucine
-
-
48.22
-
L-leucine
-
-
52
-
L-leucine
-
pH 8.5, 25C
86.6
-
L-leucine
-
-
2.6
-
L-Lys
B5U982
37C, isozyme MPLAO1
4.7
-
L-Lys
B5U982
37C, isozyme MPLAO3
318.9
-
L-Lys
-
pH 8.5, 25C
10.51
-
L-lysine
-
-
20.4
-
L-lysine
A1IGW6
; 37C
57.1
-
L-lysine
-
pH 4.0, 37C
589.3
-
L-Met
-
pH 8.5, 25C
0.21
-
L-methionine
-
pH 8.5, 25C
24.13
-
L-methionine
-
-
66.26
-
L-methionine
-
-
80.3
-
L-methionine
-
pH 8.5, 25C
142.6
-
L-Phe
-
pH 8.5, 25C
0.008
-
L-phenylalanine
-
at pH 7.0 and 20C
0.013
-
L-phenylalanine
-
at pH 7.0 and 30C
0.02
-
L-phenylalanine
-
at pH 7.0 and 50C
0.025
-
L-phenylalanine
-
at pH 7.0 and 40C
6.27
-
L-phenylalanine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
6.78
-
L-phenylalanine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
17.18
-
L-phenylalanine
-
-
48.23
-
L-phenylalanine
-
-
7.75
-
L-Propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
9.62
-
L-Propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
98.82
-
L-Trp
-
pH 8.5, 25C
6.58
-
L-tryptophan
-
-
42.6
-
L-tryptophan
-
pH 8.5, 25C
81.04
-
L-tryptophan
-
-
100.6
-
L-Tyr
-
pH 8.5, 25C
8.42
-
L-tyrosine
-
-
5.08
-
L-valine
-
-
additional information
-
additional information
-
mol amino acid/min x mol of enzyme bound FAD: 11000 L-arginine, 280 L-phenylalanine, 40 L-valine, 600 L-leucine
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.02
-
L-Ala
-
pH 7.5, 0.9% NaCl, at 25C
12025
0.94
-
L-Arg
-
pH 7.5, 0.9% NaCl, at 25C
12085
3.72
-
L-Ile
-
pH 7.5, 0.9% NaCl, at 25C
12263
21.53
-
L-Leu
-
pH 7.5, 0.9% NaCl, at 25C
12275
195
-
L-leucine
-
in 50 mM Tris-HCl buffer pH 8.0, at 37C
12291
0.03
-
L-Lys
-
pH 7.5, 0.9% NaCl, at 25C
12303
21.93
-
L-Met
-
pH 7.5, 0.9% NaCl, at 25C
12324
41.3
-
L-Phe
-
pH 7.5, 0.9% NaCl, at 25C
12354
18.02
-
L-Trp
-
pH 7.5, 0.9% NaCl, at 25C
12438
40.56
-
L-Tyr
-
pH 7.5, 0.9% NaCl, at 25C
12441
0.11
-
L-Val
-
pH 7.5, 0.9% NaCl, at 25C
12462
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01189
-
2-aminobenzoic acid
-
isoform L2, free enzyme
0.02544
-
2-aminobenzoic acide
-
isoform L2, enzyme-substrate complex
-
1.7
-
Benzoate
-
-
11
-
D-mandelate
-
-
4.5
-
Gly
Q8VPD4
pH 7.6, 30C
4.5
-
glycine
Q8VPD4
pH 7.6, 30C
221
-
L-alanine
Q8VPD4
recombinant L-AAO, expressed in and Streptomyces lividans, 30C, pH 7.6
5.42
-
L-Arg
Q8VPD4
pH 7.6, 30C
5.42
-
L-arginine
Q8VPD4
pH 7.6, 30C
20.6
-
L-citrulline
Q8VPD4
pH 7.6, 30C; pH 7.6, 30C
5.69
-
L-Leu
Q8VPD4
pH 7.6, 30C
5.69
-
L-leucine
Q8VPD4
pH 7.6, 30C
63.7
-
L-Lys
Q8VPD4
pH 7.6, 30C
63.7
-
L-lysine
Q8VPD4
pH 7.6, 30C
11
-
L-Mandelate
-
-
6.84
-
L-Met
Q8VPD4
pH 7.6, 30C
6.84
-
L-methionine
Q8VPD4
pH 7.6, 30C
2.91
-
L-Norleucine
Q8VPD4
pH 7.6, 30C; pH 7.6, 30C
12.5
-
L-Orn
Q8VPD4
pH 7.6, 30C
12.5
-
L-ornithine
Q8VPD4
pH 7.6, 30C
2.28
-
L-Phe
Q8VPD4
pH 7.6, 30C
2.28
-
L-phenylalanine
Q8VPD4
pH 7.6, 30C
7.49
-
L-Propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
9.69
-
L-Propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28C
192
-
L-Ser
Q8VPD4
pH 7.6, 30C
192
-
L-serine
Q8VPD4
pH 7.6, 30C
1.06
-
L-Tyr
Q8VPD4
pH 7.6, 30C
1.06
-
L-tyrosine
Q8VPD4
pH 7.6, 30C
1.6
-
m-Aminobenzoate
-
-
0.15
-
m-chlorobenzoate
-
-
0.37
-
m-fluorobenzoate
-
-
2.1
-
m-Hydroxybenzoate
-
-
0.3788
-
N-acetyl-L-tryptophan amide
-
isoform L2, free enzyme
0.3846
-
N-acetyl-L-tryptophan amide
-
isoform L1, enzyme-substrate complexe
0.5916
-
N-acetyl-L-tryptophan amide
-
isoform L1, free enzyme
0.7448
-
N-acetyl-L-tryptophan amide
-
isoform L2, enzyme-substrate complex
0.0935
-
N-acetyltryptophan
-
isoform L1, enzyme-substrate complex
0.1146
-
N-acetyltryptophan
-
isoform L2, free enzyme
0.1275
-
N-acetyltryptophan
-
isoform L1, free enzyme
0.2001
-
N-acetyltryptophan
-
isoform L2, enzyme-substrate complex
0.42
-
o-aminobenzoate
-
-
1
-
o-chlorobenzoate
-
-
1.4
-
o-fluorobenzoate
-
-
2.2
-
o-hydroxybenzoate
-
-
1
-
o-mercaptobenzoate
-
-
0.86
-
o-Nitrobenzoate
-
-
1.2
-
orthanilic acid
-
-
1.4
-
p-Aminobenzoate
-
-
0.7
-
p-Chlorobenzoate
-
-
1.3
-
p-fluorobenzoate
-
-
2.3
-
p-hydroxybenzoate
-
-
2.3
-
p-nitrobenzoate
-
-
0.34
-
m-Nitrobenzoate
-
-
additional information
-
additional information
Q8VPD4
wild-type L-AAO, no substrate inhibition up to 0.5 M L-alanine
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.1
-
CaCl2
Synechococcus cedrorum PCC 6908, Synechococcus elongatus PCC 7942
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.003
-
-
cell-free extract, using L-lysine as substrate, at pH 7.0 and 65C
0.01
-
-
supernatant from the culture medium
0.24
-
-
activity against L-lysine
0.38
-
-
activity against L-glutamine
0.5
-
-
activity against L-isoleucine
0.67
-
-
activity against L-tyrosine
0.81
-
-
activity against L-glutaminic acid
1.16
-
-
after 389fold purification, using L-lysine as substrate, at pH 7.0 and 65C
1.19
-
-
activity against L-histidine
1.56
-
-
crude venom
3.15
-
-
purified enzyme
3.8
-
-
substrate L-isoleucine, pH 8.5, 25C
4.4
-
-
L-threonine
4.5
-
-
substrate L-arginine, pH 8.5, 25C; substrate L-phenylalanine, pH 8.5, 25C
4.6
-
Q8VPD4
homogenous enzyme, L-alanine as susbtrate, 30C, pH 7.6
4.61
-
Q8VPD4
-
4.9
-
-
L-aspartate
5
-
-
with glycine or L-lysine as substrate
5.4
-
Q8VPD4
wild-type and recombinant enzyme; wild-type enzyme: 76fold purification, recombinant enzyme, expressed in and Streptomyces lividans: 186fold purification, 30C, pH 7.6
5.5
-
-
substrate L-histidine, pH 8.5, 25C
5.75
-
-
activity against L-arginine
6
-
-
L-glutamate
6.5
-
-
substrate L-leucine, pH 8.5, 25C
6.94
-
-
isoform L2
7.2
-
-
substrate L-tryptophan, pH 8.5, 25C
8.75
-
-
activity against L-tryptophan
8.96
-
-
isoform L1
9
-
-
purified native enzyme, pH 4.7, 37C
9.38
-
-
activity against L-phenylalanine
10
-
-
with L-glutamine as substrate
10.2
-
A1IGW6
; 37C
12.5
-
-
L-histidine
14.4
-
-
L-valine
14.4
-
-
substrate L-methionine, pH 8.5, 25C
14.8
-
-
L-tyrosine
15
-
-
activity against L-leucine
15.5
-
-
L-tryptophan
16.6
-
-
L-arginine
17
-
-
L-isoleucine
17.4
-
-
S-adenosy-L-cysteine
17.8
-
-
L-alanine
18.4
-
-
L-asparagine
18.8
-
-
L-leucine
18.9
-
-
L-lysine
20.4
-
-
L-cystine
21
-
-
activity against L-methionine
21.4
-
-
L-ornithine
24.2
-
-
L-serine
24.3
-
-
L-methionine
25.2
-
-
S-adenosyl-L-homocysteine
26
-
-
purified native enzyme, pH 4.0, 37C
26.5
-
-
L-ethionine; L-phenylalanine
30.5
-
-
L-methionine sulfoximine
35
-
-
with L-serine as substrate
39.5
-
-
L-glutamine
40
-
-
with L-threonine or L-proline as substrate
60
-
B5AR80
purified enzyme, substrate L-Ala
79.92
-
-
51.3fold purified
87.5
-
-
purified enzyme, substrate L-Leu
90
-
-
with L-arginine as substrate
110
-
B5AR80
purified enzyme, substrate L-Val
140
-
-
with L-histidine as substrate
140
-
B5AR80
purified enzyme, substrates L-Tyr or L-Glu
230
-
-
with L-alanine as substrate
300
-
-
substrate L-histidine, 25C, pH 7.2
310
-
B5AR80
purified enzyme, substrate L-Trp
335
-
B5AR80
purified enzyme, substrate L-His
340
-
B5AR80
purified enzyme, substrate L-Arg
410
-
-
substrate L-valine, 25C, pH 7.2
430
-
-
with L-valine as substrate
435
-
-
substrate L-isoleucine, 25C, pH 7.2
435
-
-
with L-isoleucine as substrate
440
-
-
substrate L-methionine, 25C, pH 7.2
460
-
-
with L-methionine as substrate
480
-
-
with L-leucine as substrate
480
-
B5AR80
purified enzyme, substrate L-Ile
487.7
-
-
12.9fold purified
545
-
B5AR80
purified enzyme, substrate L-Phe
575
-
B5AR80
purified enzyme, substrate L-Leu
580
-
-
substrate L-leucine, 25C, pH 7.2
580
-
B5AR80
purified enzyme, substrate L-Met
585
-
-
substrate L-tryptophan, 25C, pH 7.2
585
-
-
with L-tryptophan as substrate
635
-
-
with L-tyrosine as substrate
665
-
-
with L-phenylalanine as substrate
685
-
-
substrate L-tyrosine, 25C, pH 7.2
765
-
-
substrate L-phenylalanine, 25C, pH 7.2
776
-
-
L-phenylalanine
additional information
-
-
-
additional information
-
-
-
additional information
-
snake
-
enzyme assays
additional information
-
-
-
additional information
-
-
enzyme assays
additional information
-
Bothrops sp.
-
activity in 19 different genus Bothrops venoms
additional information
-
B5AR80
substrate specificity, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
-
assay at
4.7
-
-
first optimum
6.6
-
-
L-leucine
7
-
-
L-Ile, L-Leu, L-Cys, L-Met, L-Phe, L-Tyr
7
-
-, Q6IWZ0
bactericidal assay at
7
-
-
assay at
7
-
-
pH optimum for oxidation of L-lysine
7.2
7.5
Crotalus terrificus terrificus
-
2 optima, one between pH 7.2 and 7.5 and the other above pH 8.5, L-cysteine, S-adenosyl-L-homocysteine
7.2
-
B5AR80
assay at
7.2
-
-
assay at
7.4
7.8
-
S-ribosyl-L-homocysteine, S-adenosyl-L-homocysteine, S-adenosyl-L-methionine, L-methionine
7.4
-
-, Q90W54
assay at
7.5
-
-
L-leucine
7.6
-
Q8VPD4
assay at
8
8.4
Crotalus terrificus terrificus
-
homocysteine, cysteine
8
8.5
Bacillus carotarum
-
L-phenylalanine
8
9
Q8VPD4
; for 10 mM L-alanine, L-leucine, L-phenylalanine, the pH-profiles are almost identical
8
-
-
L-lactic acid
8.1
-
-
assay at
8.5
8.8
-
djenkolic acid
8.5
-
Crotalus terrificus terrificus
-
2 optima, one between pH 7.2 and 7.5 and the other above pH 8.5, L-cysteine, S-adenosylhomocysteine, with S-adenosyl-homocysteine and djenkolic acid increase of activity beyond pH 8.5
8.5
-
-
assay at
8.5
-
-
second optimum
8.5
-
-
assay at
8.5
-
-
assay at
8.7
-
-
L-leucine
8.8
9.2
-
S-adenosyl-L-homocysteine
9
-
-
broad optimum
9
-
-
pH optimum for oxidation of L-ornithine and L-arginine
9.5
-
-
L-phenylalanine
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
12
-
half maximal activity at pH 3.5 and 12
4
11
Q8VPD4
L-phenylalanine as substrate, pH 4: ca. 10% of the activity, pH 11: ca. 60% of the activity
4.5
10.5
Bacillus carotarum
-
-
4.5
10.5
Q8VPD4
L-alanine as substrate, pH 4.5, ca. 3% of the activity, pH 10.5, ca. 30% of the activity
4.5
8.5
-, Q6IWZ0
pH-profile, overview
5.5
8.5
-
activity assay range
6
10
Hebeloma sp.
-
-
7
9
-
about 55% activity at pH 7.0, about 85% activity at pH 9.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
B5AR80
assay at
25
-
-, Q6IWZ0
assay at
25
-
-
assay at
25
-
Q4F867
assay at
25
-
-
assay at
30
-
Q8VPD4
assay at
30
-
-
assay at
37
-
-, Q6IWZ0
bactericidal assay at
37
-
-, Q90W54
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
40
-
-
at pH 7.0
49
-
-
L-phenylalanine
50
60
-
temperature optimum for for oxidation of L-arginine
50
-
Bacillus carotarum
-
-
50
-
-
temperature optimum for for oxidation of L-ornithine
65
-
-
temperature optimum for oxidation of L-lysine
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
37
-
almost inactive at 0C and 37C
35
45
-
decrease of activity to 35-50% and 90-78% of maximal activity at 20-30C and 45-50C, respectively
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.4
-
-
isoelectric focussing
4.6
-
-, Q6T277
calculated
4.6
-
-
isoelectric focusing
4.7
-
-
isoelectric focusing
4.8
-
Q8VPD4
isoelectric focusing, using a narrow pH-range of 4-6
4.9
-
Q8VPD4
-
4.9
-
-
isoelectric focusing
4.96
-
B5U982
mature MPLAO3, sequence calculation
5
-
Q6TGQ9
-
5.3
-
D2KWB6
isoelectric focusing
5.43
-
-
isoelectric focusing
5.63
-
Q6TGQ9
estimated
5.7
-
-
isoelectric focusing
5.8
-
Q6TGQ8
estimated
6.3
-
B5AR80
sequence calculation
6.3
-
-
isoelectric focusing
6.9
-
-
-
8
-
-
isoelectric focusing
8
-
-
calculated from amino acid sequence
8.6
-
-
calculated from amino acid sequence
9.4
-
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
most primary mediastinal B-cell lymphoma (89%) are positive for enzyme immunostaining with strong intracytoplasmic staining in up to 100% of tumor cells, CD19(+) and CD19(-) cells display enzyme activity
Manually annotated by BRENDA team
-
64% positive immunostaining, no correlation between staining and histological grade of the follicular lymphoma, in small lymphocytic lymphoma 40% positive, restricted to paraimmunoblasts of proliferative centers, high enzyme activity, 7fold higher than in spleen lysate
Manually annotated by BRENDA team
-
dominantly expressed
Manually annotated by BRENDA team
-
variable percentage of positive staining in several tissues, solid tumors, interfollicular areas, sinuses of reactive lymph nodes, red pulp of lymphoid follicles, thymus medulla, all lymphoma subtypes, particularly high enzyme expression in classical Hodgkin lymphoma and nodular lymphocyte predominant Hodgkin lymphoma cells
Manually annotated by BRENDA team
Aplysia californica Cooper 1863
-
-
-
Manually annotated by BRENDA team
-
weakly expressed
Manually annotated by BRENDA team
-
42% positive staining of classical Hodgkin lymphoma in Hodgkin and Reed Sternberg cells, 91% positive staining of nodular lymphocyte predominant Hodgkin lymphoma cells
Manually annotated by BRENDA team
-
immunostaining in macrophages associated with non small-cell lung carcinomas, mesotheliomas, colorectal carcinomas and testicular germ cell tumors, positive staining in germinal centers of reactive lymph nodes and hyperplastic tonsils, and in the spleen
Manually annotated by BRENDA team
-
lactating, tissue-specific LAO
Manually annotated by BRENDA team
Hebeloma sp.
-
-
Manually annotated by BRENDA team
-
weakly expressed
Manually annotated by BRENDA team
A1IGW6
mucus; skin mucus
Manually annotated by BRENDA team
-
mucus, dominantly expressed. Quantity of SSAP mRNA in skin varies among individuals, ranging from 1.1 to 13.9 nanog/microg total RNA, although no relationship is found between the size of fish and gene expression
Manually annotated by BRENDA team
B5U982
isozymes MPLAO1, MPLAO2, and MPLAO3
Manually annotated by BRENDA team
Crotalus terrificus terrificus, Providencia rettgeri
-
-
Manually annotated by BRENDA team
-
five LAAO isozymes
Manually annotated by BRENDA team
additional information
-
no expression in muscle, stomach, intestine, liver and spleen
Manually annotated by BRENDA team
additional information
-
enzyme expression in solid tumors only in 7.4% of 121 cases (5/10 mesotheliomas, 2/13 non small-cell carcinomas, 1/6 thyroid carcinoma, and 1/3 ovarian carcinoma), observations suggest aril in inflammatory processes, only occasional positive staining (17%) of non-mediastinal diffuse large B cell lymphoma; strong enzyme activity in L428 cell-line associated with detectable enzyme secretion, higher enzymatic activity than in SU-DHL-4 cells, higher enzyme activity in KM-H2 cell line than in Su-DHL-4 cells but lower than in L428 cells, total tumor cell lysate with negligible enzyme activity, CD19(+) fraction of marginal zone lymphoma cell with negligible enzyme activity, some activity in CD19(-) stromal cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
B5U982
skin mucus, isozyme MPLAO3 contains a signal peptide, comprising residues Met1-Ala26
-
Manually annotated by BRENDA team
Aplysia californica Cooper 1863
-
defensive ink
-
-
Manually annotated by BRENDA team
-
enzyme is part of photosystem II particles
Manually annotated by BRENDA team
Synechococcus cedrorum PCC 6908
-
-
-
Manually annotated by BRENDA team
Synechococcus cedrorum PCC 6908
-
-
-
Manually annotated by BRENDA team
Corynebacterium sp. A20
-
-
-
-
Manually annotated by BRENDA team
Synechococcus cedrorum PCC 6908
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
12000
-
-
gel filtration
53000
-
-
Western blotting
53780
-
Q6TGQ8
sequence analysis
54130
-
P72346
sequence analysis
54760
-
Q6TGQ9
sequence analysis
54770
-
-
sequence analysis
59000
-
-
matrix-assisted laser desorption/ionization mass spectroscopy
60000
-
-, Q6IWZ0
gel filtration
60700
-
-
MALDI-TOF mass spectrometry
68000
-
-
gel filtration
68000
-
-
HPLC
70000
-
-
gel filtration
96000
-
-
gel filtration, mass spectrometry
98000
-
-
gel filtration
99000
-
Q8VPD4
gel filtartion; gel filtration
102000
115000
Bacillus carotarum
-
gel filtration, native PAGE
104000
-
Q8VPD4
MALDI-TOF/MS
108800
-
-
gel filtration
110000
-
-
gel filtration
110000
-
-
gel filtration
110000
-
-
gel filtration
112000
-
-
-
113000
-
O09046
gel filtration
113000
-
-
mammary gland-specific isozyme, gel filtration
120000
-
-
gel filtration
120000
-
A1IGW6
gel filtration; gel filtration
120000
-
Q4F867
gel filtration
120000
-
-
gel filtration
124400
-
-
gel filtration
125000
-
Q6TGQ9
gel filtration
128000
153000
-
approach to equilibrium method
130000
140000
-
gel filtration
130000
140000
-
sedimentation equilibrium
130000
-
-
gel filtration
130800
-
-
mass spectrometry
132000
-
-
gel filtration
135000
-
-
-
135000
-
-
gel filtration
138000
-
-
calculation from diffusion constant, sedimentation coefficient and FAD content
140000
-
-
gel filtration
140000
-
-
gel filtration
140000
-
-
or above, gel filtration
140000
-
B5U982
isozyme MPLAO3, gel filtration
145000
-
B5U982
isozyme MPLAO1, gel filtration
150000
-
-
approach to equilibrium method
150000
-
-
gel filtration
160000
-
-
gel filtration
280000
-
B5U982
isozyme MPLAO2, peak 1, gel filtration
300000
-
-
disc gel electrophoresis, linear acrylamide concentration
310000
314000
-
high speed equilibrium method, gel filtration
600000
-
B5U982
isozyme MPLAO2, peak 2, gel filtration
900000
1000000
-
form Malpha, gel filtration, sedimentation equilibrium
1200000
1300000
-
form Mbeta, gel filtration, sedimentation equilibrium
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q8VPD4
x * 53200, SDS-PAGE
?
-
x * 62000, SDS-PAGE
?
-, Q6T277
x * 60000, SDS-PAGE, x * 60167, calculated
?
-
x * 43000, SDS-PAGE
?
-
x * 55000, LAAO I, SDS-PAGE
?
-
x * 67000, SDS-PAGE
?
-
x * 58000, mammary gland-specific isozyme, SDS-PAGE
?
-, Q90W54
x * 60000, SDS-PAGE
?
-
x * 38200, deglycosylated enzyme, SDS-PAGE
?
-
x * 60000, SDS-PAGE
?
D2KWB6
x * 52000, SDS-PAGE, 6 m urea ?PAGE, and 2D-PAGE
?
-
x * 72000, SDS-PAGE
?
-
x * 54864, calculated from amino acid sequence; x * 62025, MALDI-TOF mass spectrometry; x * 63600, SDS-PAGE
?
Pseudoalteromonas flavipulchra C2
-
x * 60000, SDS-PAGE
-
dimer
-
2 * 68000, SDS-PAGE
dimer
-
2 * 70000, SDS-PAGE
dimer
-
2 * 69000
dimer
-
2 * 66000
dimer
-
2 * 58700
dimer
-
2 * 69000
dimer
-
2 * 66000
dimer
-
2 * 57400
dimer
-
2 * 65000
dimer
-
2 * 56000
dimer
-
2 * 70000, sedimentation equilibrium in presence of 6 M guanidine hydrochloride, 2 types of polypeptide chains
dimer
-
2 * 49000, SDS-PAGE
dimer
O09046
2 * 60000, SDS-PAGE
dimer
-
2 * 66000, SDS-PAGE
dimer
-
2 * 60000, SDS-PAGE
dimer
Bacillus carotarum
-
2 * 54000, SDS-PAGE
dimer
-
2 * 65000-70000, SDS-PAGE
dimer
Q8VPD4
2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules
dimer
-
2 * 66000, SDS-PAGE
dimer
-
2 * 55000, SDS-PAGE
dimer
-
2 * 542000, calculated and crystallization data
dimer
-
2 * 66000, SDS-PAGE
dimer
A1IGW6
2 * 53000, SDS-PAGE
dimer
-
2 * 60900, MALDI-TOF, 2 * 66000, SDS-PAGE
dimer
-
gel filtration
dimer
-
2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions
dimer
B5U982
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation
dimer
Bacillus carotarum 2Pfa
-
2 * 54000, SDS-PAGE
-
dimer or trimer
-
x * 53000, SDS-APGE
homodimer
A1IGW6
2 * 53000, SDS-PAGE, 2 * 55261, sequence analysis
homodimer
-
high-resolution
homodimer
-
2 * 60000, SDS-PAGE
homodimer
Q6TGQ9
2 * 60000, SDS-PAGE
homodimer
-
2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE
homodimer
-
SDS-PAGE
homodimer
B5AR80
2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation
homodimer
Q4F867
2 * 58000, SDS-PAGE
homodimer
-
2 * 65000, SDS-PAGE
homodimer
-
2 * 54500, SDS-PAGE
homodimer
-
2 * 56538, calculated from sequence of cDNA; 2 * 60000, SDS-PAGE; 2 * 60852, mass spectrometry
homodimer
-
2 * 54500, SDS-PAGE
-
monomer
-, Q6IWZ0
1 * 60000, SDS-PAGE
monomer
-
1 * 110000, SDS-PAGE
monomer
-
1 * 60000, SDS-PAGE
monomer
-
1 * 57000, SDS-PAGE
monomer
-
1 * 60000-63000, purified isoforms L1 and L2, size exclusion HPLC and SDS-PAGE
monomer
-
1 * 53000, SDS-PAGE
monomer
A8QL50, -
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis
monomer
-
1 * 55000, SDS-PAGE
monomer
-
1 * 55000, SDS-PAGE; 1 * 56827, calculated from amino acid sequence
oligomer
-
Malpha, ? * 66000, Mbeta, ? * 66000 + ? * 135000, latter polypeptide not required for amino acid oxidase activity
monomer
Pseudoalteromonas luteoviolacea CPMOR-1, Pseudoalteromonas luteoviolacea CPMOR-2, Pseudoalteromonas luteoviolacea NCIMB 1893T
-
1 * 110000, SDS-PAGE
-
additional information
snake
-
overview on structural properties
additional information
-
peptide mapping by mass spectrometry
additional information
B5AR80
structure analysis and molecular dynamics simulation, overview
additional information
-
peptide mapping
additional information
B5U982
isozyme MPLAO3 contains a signal peptide, comprising residues Met1-Ala26, and the mature protein, Val28-Phe520, peptide mapping and sequencing of isozymes MPLAO3 and MPLAO1
additional information
-
toxophallin peptide mapping and sequence analysis by MALDI-TOF mass spectrometry, overview
additional information
-
LAAO comprises the FAD-binding domain, the substrate binding domain, and the alpha-helical domain, the substrate binding domain is made up by residues 5-25, 73-129, 233-236 and 323-420, and contains 4 alpha-helices, formed by residues 16-25, 96-104, 375-383 and 386-401, and 11 beta-strands, residues 74-77, 82-85, 109-112, 120-123, 126-129, 233-236, 324-331, 345-347, 354-356, 367-374 and 412-419
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-, Q6IWZ0
sequence contains one potential glycosylation site. Glycosylation is not essential for antimicrobial activity
proteolytic modification
-, Q6IWZ0
sequence contains a signal peptide of 18 amino acids
glycoprotein
-, Q6T277
weakly glycosylated, sequence lacks classical N-glycosylation sites
flavoprotein
B5AR80
-
glycoprotein
B5AR80
-
glycoprotein
-
deglycosylation does not alter the enzymatic activity of the acid glycoprotein
glycoprotein
-
-
flavoprotein
-
-
glycoprotein
-
-
glycoprotein
-
deglycosylation does not alter enzymatic activity
glycoprotein
-
glycan is a bis-sialylated, biantennary, core-fucosylated dodecasaccharide
glycoprotein
-
desialysation reduces cytotoxicity of enzyme. Glycan moiety of enzyme is involved in binding and internalization to Jurkat cells
glycoprotein
-
both polypeptide chains are glycosylated
glycoprotein
-
2-5% carbohydrate, including sialic acid
glycoprotein
-
the enzyme has three potential glycosylation sites at Asn 172, 194, and 361