Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Lachesis muta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
L-leucine | at pH 8.0, temperature not specified in the publication | Lachesis muta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56538 | - |
2 * 56538, calculated from sequence of cDNA | Lachesis muta |
60000 | - |
2 * 60000, SDS-PAGE | Lachesis muta |
60852 | - |
2 * 60852, mass spectrometry | Lachesis muta |
120000 | - |
gel filtration | Lachesis muta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lachesis muta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Mono Q column chromatography and Sephacryl S-100 gel filtration or phenyl Sepharose column chromatography, Affi-gel column chromatography, and Sephacryl S-200 gel filtration | Lachesis muta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Lachesis muta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O + O2 | about 10% activity compared to L-methionine | Lachesis muta | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-glutamine + H2O + O2 | about 5% activity compared to L-methionine | Lachesis muta | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
L-isoleucine + H2O + O2 | about 35% activity compared to L-methionine | Lachesis muta | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | about 99% activity compared to L-methionine | Lachesis muta | 4-methyl-2-oxo-pentanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | 100% activity | Lachesis muta | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | about 55% activity compared to L-methionine | Lachesis muta | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | about 55% activity compared to L-methionine | Lachesis muta | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | about 40% activity compared to L-methionine | Lachesis muta | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
L-valine + H2O + O2 | less than 10% activity compared to L-methionine | Lachesis muta | 2-oxoisovalerate + NH3 + H2O2 | - |
? | |
additional information | no activity with L-lysine, L-cysteine, L-serine, L-threonine, and L-cystine | Lachesis muta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Lachesis muta |
homodimer | 2 * 56538, calculated from sequence of cDNA | Lachesis muta |
homodimer | 2 * 60852, mass spectrometry | Lachesis muta |
Synonyms | Comment | Organism |
---|---|---|
L-amino acid oxidase | - |
Lachesis muta |
LAAO | - |
Lachesis muta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Lachesis muta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 55% activity at pH 7.0, about 85% activity at pH 9.0 | Lachesis muta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Lachesis muta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lachesis muta | isoelectric focusing | - |
6.3 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme induces cytotoxicity on AGS cell line (gastric adenocarcinoma) and on MCF-7 cell line (breast adenocarcinoma). It presents antiparasitic activity on Leishmania brasiliensis, but Trypanosoma cruzi is resistant to this enzyme | Lachesis muta |