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Literature summary for 1.4.3.2 extracted from

  • Singh, S.; Gogoi, B.; Bezbaruah, R.
    Aspergillus fumigatus L-amino acid oxidase-two step purification and characterization of the enzyme (2014), J. Microb. Biochem. Technol., 6, 96-101 .
No PubMed abstract available
Search for more literature for 1.4.3.2

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 70.07% inhibition at 10 mM Aspergillus fumigatus
alpha-Naphthol 12.4% inhibition at 10 mM Aspergillus fumigatus
benzoic acid complete inhibition at 10 mM Aspergillus fumigatus
Ca2+
-
 Aspergillus fumigatus
EDTA complete inhibition at 10 mM Aspergillus fumigatus
FeSO4
-
 Aspergillus fumigatus
glycine 29.56% inhibition at 10 mM Aspergillus fumigatus
H3BO3
-
 Aspergillus fumigatus
Na2MO4
-
 Aspergillus fumigatus
riboflavin 44.89% inhibition at 10 mM Aspergillus fumigatus
Sodium azide 49.63% inhibition at 10 mM Aspergillus fumigatus
Zn2+
-
 Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
43.47
-
 DL-Ala at pH 7.2 and 30°C Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ Cu2+ at 10 mM increases the enzyme activity by 1.65fold Aspergillus fumigatus
Mg2+ Mg2+ at 1 mM and 10 mM increased the enzyme activity by 1.72 and 2.22fold respectively Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
 gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sephadex A-50 column chromatography, and Sephadex G-75 gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-Ala + H2O + O2 42% activity compared to DL-Tyr Aspergillus fumigatus 2-oxopropanoic acid + NH3 + H2O2
-
 ?
DL-Leu + H2O + O2 55.6% activity compared to DL-Tyr Aspergillus fumigatus 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
DL-Tyr + H2O + O2 best substrates Aspergillus fumigatus 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
L-Ala + H2O + O2 54.1% activity compared to DL-Tyr Aspergillus fumigatus 2-oxopropanoic acid + NH3 + H2O2
-
 ?
L-Asp + H2O + O2 13.2% activity compared to DL-Tyr Aspergillus fumigatus oxaloacetate + NH3 + H2O2
-
 ?
L-Phe + H2O + O2 76% activity compared to DL-Tyr Aspergillus fumigatus phenylpyruvate + NH3 + H2O2
-
 ?
L-Ser + H2O + O2 66% activity compared to DL-Tyr Aspergillus fumigatus 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
additional information L-amino acids like L-threonine, L-cystin, L-tryptophan, L-glutamic acid are not attacked by the enzyme. Also there is no activity against the basic amino acid L-arginine, and no enzyme activity is detected with the D-amino acids Aspergillus fumigatus ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 55000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
L-AAO
-
 Aspergillus fumigatus
L-amino acid: O2 oxidoreductase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 35 the enzyme activity is 88.78% and 41.98% at 25°C and 35°C, respectively Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 90 the enzyme is stable over temperatures up to 40°C although loss in enzyme activity is seen. Treatment of the enzyme at 40 and 50°C for 5 minutes causes 38.47% and 67.19% loss in activity, respectively, compared to the untreated enzyme. When the time of incubation of the enzyme at 40 and 50°C is increased to 10 min, total loss of enzyme activity occurs. Treatment of the enzyme at higher temperatures of 60-90°C for 5 min completely inactivates the enzyme Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Aspergillus fumigatus