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Literature summary for 1.4.3.2 extracted from
- Purification and characterization of L-amino acid oxidase from the venom of Trimeresurus mucrosquamatus (Taiwan habu snake) (1988), Toxicon, 26, 695-706.
General Stability
| General Stability | Organism |
|---|---|
| freezing, irreversible inactivation | Protobothrops mucrosquamatus |
| irreversible inactivation by lyophilization | Protobothrops mucrosquamatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzoic acid | - |
Protobothrops mucrosquamatus |  |
| Cd2+ | CdCl2 | Protobothrops mucrosquamatus | |
| HgCl | - |
Protobothrops mucrosquamatus | |
| iodoacetamide | - |
Protobothrops mucrosquamatus | |
| KCN | - |
Protobothrops mucrosquamatus | |
| MnCl2 | - |
Protobothrops mucrosquamatus | |
| p-Aminobenzoic acid | - |
Protobothrops mucrosquamatus | |
| p-chloromercuribenzoate | - |
Protobothrops mucrosquamatus | |
| ZnCl2 | - |
Protobothrops mucrosquamatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.17 | - |
L-leucine | - |
Protobothrops mucrosquamatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | MnCl2 inhibits | Protobothrops mucrosquamatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
2 * 70000, SDS-PAGE | Protobothrops mucrosquamatus |
| 140000 | - |
gel filtration | Protobothrops mucrosquamatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Protobothrops mucrosquamatus | - |
Taiwan habu snake | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Protobothrops mucrosquamatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Protobothrops mucrosquamatus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 40% v/v glycerol, 3 months | Protobothrops mucrosquamatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-amino acid + H2O + O2 | - |
Protobothrops mucrosquamatus | 2-oxo acid + NH3 + H2O2 | - |
? | |
| L-arginine + H2O + O2 | low reaction rate | Protobothrops mucrosquamatus | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
| L-asparagine + H2O + O2 | very low activity | Protobothrops mucrosquamatus | alpha-ketosuccinamic acid + NH3 + H2O2 | - |
? | |
| L-aspartic acid + H2O + O2 | low activity | Protobothrops mucrosquamatus | oxaloacetate + NH3 + H2O2 | - |
? | |
| L-cystine + H2O + O2 | very low activity | Protobothrops mucrosquamatus | ? + NH3 + H2O2 | - |
? | |
| L-glutamic acid + H2O + O2 | low activity | Protobothrops mucrosquamatus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| L-glutamine + H2O + O2 | very low activity | Protobothrops mucrosquamatus | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
| L-histidine + H2O + O2 | low reaction rate | Protobothrops mucrosquamatus | 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2 | - |
? | |
| L-isoleucine + H2O + O2 | low reaction rate | Protobothrops mucrosquamatus | alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2 | - |
? | |
| L-leucine + H2O + O2 | high reaction rate | Protobothrops mucrosquamatus | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
| L-methionine + H2O + O2 | high reaction rate | Protobothrops mucrosquamatus | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
| L-phenylalanine + H2O + O2 | high reaction rate | Protobothrops mucrosquamatus | phenylpyruvate + NH3 + H2O2 | - |
? | |
| L-tryptophan + H2O + O2 | low reaction rate | Protobothrops mucrosquamatus | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
| L-tyrosine + H2O + O2 | - |
Protobothrops mucrosquamatus | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
| L-valine + H2O + O2 | very low activity | Protobothrops mucrosquamatus | 2-oxoisovalerate + NH3 + H2O2 | - |
? | |
| additional information | not: proline | Protobothrops mucrosquamatus | ? | - |
? | |
| additional information | not: L-threonine | Protobothrops mucrosquamatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 70000, SDS-PAGE | Protobothrops mucrosquamatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, pH 6.8, stable up to | Protobothrops mucrosquamatus |
| 65 | - |
10 min, 54% loss of activity | Protobothrops mucrosquamatus |
| 75 | - |
10 min, 97% loss of activity | Protobothrops mucrosquamatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
L-Ile, L-Leu, L-Cys, L-Met, L-Phe, L-Tyr | Protobothrops mucrosquamatus |
| 8 | - |
L-His | Protobothrops mucrosquamatus |
| 9 | - |
L-Trp | Protobothrops mucrosquamatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
4°C, 24 h, complete inactivation | Protobothrops mucrosquamatus |
| 7 | 8 | 4°C, 24 h, stable | Protobothrops mucrosquamatus |
| 10 | - |
4°C, 24 h, complete inactivation | Protobothrops mucrosquamatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | 2 mol of FMN per mol of enzyme | Protobothrops mucrosquamatus | |
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