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Literature summary for 1.4.3.2 extracted from

  • Samel, M.; Tonismaegi, K.; Roennholm, G.; Vija, H.; Siigur, J.; Kalkkinen, N.; Siigur, E.
    L-Amino acid oxidase from Naja naja oxiana venom (2008), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 149, 572-580.
    View publication on PubMed

Application

Application Comment Organism
additional information the enzyme has antibacterial activity inhibiting the growth of Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria. LAAO dose-dependently inhibits ADP- or collagen-induced platelet aggregation with IC50 of 0.094 micromol and 0.036 micromol, respectively Naja oxiana

Inhibitors

Inhibitors Comment Organism Structure
additional information the antibacterial and anti-aggregating activity of LAAO is abolished by catalase Naja oxiana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
L-phenylalanine
-
Naja oxiana
0.147
-
L-tryptophan
-
Naja oxiana
0.75
-
L-leucine
-
Naja oxiana
0.885
-
L-methionine
-
Naja oxiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
1 * 57000, SDS-PAGE Naja oxiana
110000
-
gel filtration Naja oxiana

Organism

Organism UniProt Comment Textmining
Naja oxiana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, ion exchange chromatography, 100fold purified Naja oxiana

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Naja oxiana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.24
-
activity against L-lysine Naja oxiana
0.38
-
activity against L-glutamine Naja oxiana
0.5
-
activity against L-isoleucine Naja oxiana
0.67
-
activity against L-tyrosine Naja oxiana
0.81
-
activity against L-glutaminic acid Naja oxiana
1.19
-
activity against L-histidine Naja oxiana
5.75
-
activity against L-arginine Naja oxiana
8.75
-
activity against L-tryptophan Naja oxiana
9.38
-
activity against L-phenylalanine Naja oxiana
15
-
activity against L-leucine Naja oxiana
21
-
activity against L-methionine Naja oxiana

Storage Stability

Storage Stability Organism
4°C, several months Naja oxiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O + O2 good substrate Naja oxiana 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
L-leucine + H2O + O2 good substrate Naja oxiana 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2 good substrate Naja oxiana 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2 good substrate Naja oxiana phenylpyruvate + NH3 + H2O2
-
?
L-tryptophan + H2O + O2 good substrate Naja oxiana 3-indole-2-oxopropanoate + NH3 + H2O2
-
?
additional information L-histidine, L-glutaminic acid, L-tyrosine, L-isoleucine, L-glutamine and L-lysine are poor substrates Naja oxiana ?
-
?

Subunits

Subunits Comment Organism
dimer gel filtration Naja oxiana
monomer 1 * 57000, SDS-PAGE Naja oxiana

Synonyms

Synonyms Comment Organism
LAAO
-
Naja oxiana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-70
-
the enzyme is stable at low temperatures (-20°C, -70°C) for one week and completely loses its activity after heating at 70°C for 10 min Naja oxiana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.18
-
L-phenylalanine
-
Naja oxiana
47.98
-
L-leucine
-
Naja oxiana
66.26
-
L-methionine
-
Naja oxiana
81.04
-
L-tryptophan
-
Naja oxiana

pI Value

Organism Comment pI Value Maximum pI Value
Naja oxiana isoelectric focusing
-
8