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Literature summary for 1.4.3.2 extracted from

  • Nagashima, Y.; Tsukamoto, C.; Kitani, Y.; Ishizaki, S.; Nagai, H.; Yanagimoto, T.
    Isolation and cDNA cloning of an antibacterial L-amino acid oxidase from the skin mucus of the great sculpin Myoxocephalus polyacanthocephalus (2009), Comp. Biochem. Physiol. B, 154, 55-61.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozymes MPLAO1, MPLAO2, and MPLAO3, DNA and amino acid sequence determinhation and analysis, phylogenetic analysis and sequence comparisons Myoxocephalus polyacanthocephalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
L-Lys 37°C, isozymes MPLAO1 and MPLAO3 Myoxocephalus polyacanthocephalus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular skin mucus, isozyme MPLAO3 contains a signal peptide, comprising residues Met1-Ala26 Myoxocephalus polyacanthocephalus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54769
-
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation Myoxocephalus polyacanthocephalus
56000
-
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation Myoxocephalus polyacanthocephalus
58000
-
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation Myoxocephalus polyacanthocephalus
140000
-
isozyme MPLAO3, gel filtration Myoxocephalus polyacanthocephalus
145000
-
isozyme MPLAO1, gel filtration Myoxocephalus polyacanthocephalus
280000
-
isozyme MPLAO2, peak 1, gel filtration Myoxocephalus polyacanthocephalus
600000
-
isozyme MPLAO2, peak 2, gel filtration Myoxocephalus polyacanthocephalus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Lys + H2O + O2 Myoxocephalus polyacanthocephalus
-
6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
?
additional information Myoxocephalus polyacanthocephalus MPLAO3 exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, and is most active against Aeromonas salmonicida strain JCM7874, which is attributable to H2O2, because the activity is completely lost in the presence of catalase ?
-
?

Organism

Organism UniProt Comment Textmining
Myoxocephalus polyacanthocephalus B5U982 isozymes MPLAO1, MPLAO2, and MPLAO3
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes by lectin affinity and anion exchange chromatography, isozyme MPLAO1 and MPLAO3 are purified to homogeneity, while isozyme MPLAO2 is not Myoxocephalus polyacanthocephalus

Source Tissue

Source Tissue Comment Organism Textmining
skin mucus isozymes MPLAO1, MPLAO2, and MPLAO3 Myoxocephalus polyacanthocephalus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Lys + H2O + O2
-
Myoxocephalus polyacanthocephalus 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
?
additional information MPLAO3 exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, and is most active against Aeromonas salmonicida strain JCM7874, which is attributable to H2O2, because the activity is completely lost in the presence of catalase Myoxocephalus polyacanthocephalus ?
-
?

Subunits

Subunits Comment Organism
dimer x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation Myoxocephalus polyacanthocephalus
More isozyme MPLAO3 contains a signal peptide, comprising residues Met1-Ala26, and the mature protein, Val28-Phe520, peptide mapping and sequencing of isozymes MPLAO3 and MPLAO1 Myoxocephalus polyacanthocephalus

Synonyms

Synonyms Comment Organism
LAO
-
Myoxocephalus polyacanthocephalus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Myoxocephalus polyacanthocephalus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
L-Lys 37°C, isozyme MPLAO1 Myoxocephalus polyacanthocephalus
4.7
-
L-Lys 37°C, isozyme MPLAO3 Myoxocephalus polyacanthocephalus

pI Value

Organism Comment pI Value Maximum pI Value
Myoxocephalus polyacanthocephalus mature MPLAO3, sequence calculation
-
4.96

General Information

General Information Comment Organism
physiological function the antibacterial LAO isozymes may be involved in the innate immunity of the great sculpin Myoxocephalus polyacanthocephalus skin Myoxocephalus polyacanthocephalus