Crystallization (Comment) | Organism |
---|---|
structures of L-AAO/MOG complexed with L-Lys, L-ornithine, and L-Arg. Residue Asp238 is located at the ceiling of a long hydrophobic pocket and forms a strong interaction with the terminal, positively charged group of the substrates. In the ligand-free structure, there are two channels connecting the active site and solvent, and a short region located at the dimer interface is disordered. In the L-Lys complex structure, a loop region is displaced to plug the channels | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
D238A | the interaction of Asp238 with the terminal, positively charged group of the substratesis critical for substrate binding but not for catalytic control between the oxidase/monooxygenase activities | Pseudomonas sp. |
D238E | mutant displays increased catalytic activities | Pseudomonas sp. |
D238F | mutant exhibits altered substrate specificity to long hydrophobic substrates | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
L-lysine | mutant D238E, pH 7, 30°C | Pseudomonas sp. | |
0.022 | - |
L-ornithine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
0.027 | - |
L-lysine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
0.041 | - |
L-ornithine | mutant D238E, pH 7, 30°C | Pseudomonas sp. | |
0.048 | - |
L-arginine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
0.059 | - |
L-lysine | mutant D238F, pH 7, 30°C | Pseudomonas sp. | |
0.08 | - |
L-arginine | mutant D238F, pH 7, 30°C | Pseudomonas sp. | |
0.41 | - |
L-arginine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.45 | - |
L-ornithine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.61 | - |
L-lysine | mutant D238A, pH 7, 30°C | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | W6JQJ6 | bifunctional L-amino acid oxidase/monooxygenase, cf. EC. 1.13.12.2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O + O2 | - |
Pseudomonas sp. | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-lysine + H2O + O2 | - |
Pseudomonas sp. | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
L-ornithine + H2O + O2 | - |
Pseudomonas sp. | 5-amino-2-oxopentanoate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-AAO/MOG | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-arginine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.1 | - |
L-arginine | mutant D238F, pH 7, 30°C | Pseudomonas sp. | |
0.17 | - |
L-lysine | mutant D238F, pH 7, 30°C | Pseudomonas sp. | |
0.37 | - |
L-ornithine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.41 | - |
L-arginine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
0.5 | - |
L-lysine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.58 | - |
L-ornithine | mutant D238E, pH 7, 30°C | Pseudomonas sp. | |
0.8 | - |
L-lysine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
1.5 | - |
L-ornithine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
2.3 | - |
L-lysine | mutant D238E, pH 7, 30°C | Pseudomonas sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-arginine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.82 | - |
L-lysine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
0.82 | - |
L-ornithine | mutant D238A, pH 7, 30°C | Pseudomonas sp. | |
1.3 | - |
L-arginine | mutant D238F, pH 7, 30°C | Pseudomonas sp. | |
3.5 | - |
L-lysine | mutant D238F, pH 7, 30°C | Pseudomonas sp. | |
8.5 | - |
L-arginine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
14 | - |
L-ornithine | mutant D238E, pH 7, 30°C | Pseudomonas sp. | |
30 | - |
L-lysine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
68 | - |
L-ornithine | wild-type, pH 7, 30°C | Pseudomonas sp. | |
130 | - |
L-lysine | mutant D238E, pH 7, 30°C | Pseudomonas sp. |