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Literature summary for 1.4.3.2 extracted from

  • Abdelkafi-Koubaa, Z.; Jebali, J.; Othman, H.; Morjen, M.; Aissa, I.; Zouari-Kesentini, R.; Bazaa, A.; Ellefi, A.A.; Majdoub, H.; Srairi-Abid, N.; Gargouri, Y.; El Ayeb, M.; Marrakchi, N.
    A thermoactive L-amino acid oxidase from Cerastes cerastes snake venom purification, biochemical and molecular characterization (2014), Toxicon, 89, 32-44 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
N-acetyl-L-tryptophan about 90% inhibition at 50 nM Cerastes cerastes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
L-Phe at pH 7.8 and 50°C Cerastes cerastes
0.1
-
L-Met at pH 7.8 and 50°C Cerastes cerastes
0.25
-
L-Leu at pH 7.8 and 50°C Cerastes cerastes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
gel filtration Cerastes cerastes

Organism

Organism UniProt Comment Textmining
Cerastes cerastes
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Cerastes cerastes

Purification (Commentary)

Purification (Comment) Organism
Resource Q column chromatography, HiTrap Heparin column chromatograph, yand Sephadex G-75 gel filtration Cerastes cerastes

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Cerastes cerastes
-

Storage Stability

Storage Stability Organism
-20°C, 24 h, 85% loss of activity Cerastes cerastes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu + H2O + O2
-
Cerastes cerastes 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
?
L-Met + H2O + O2
-
Cerastes cerastes 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-Phe + H2O + O2
-
Cerastes cerastes phenylpyruvate + NH3 + H2O2
-
?
additional information casein is not a substrate Cerastes cerastes ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 58000, SDS-PAGE Cerastes cerastes
homodimer 2 * 56573, calculated from amino acid sequence Cerastes cerastes

Synonyms

Synonyms Comment Organism
L-amino acid: O2 oxidoreductase
-
Cerastes cerastes
LAAO
-
Cerastes cerastes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Cerastes cerastes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 after 120 min incubation, the enzyme retains 80% of its activity at 50°C. At a temperature over 55°C, the activity decreases dramatically to reach 10% at 60°C Cerastes cerastes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.5
-
L-Met at pH 7.8 and 50°C Cerastes cerastes
24.4
-
L-Leu at pH 7.8 and 50°C Cerastes cerastes
31.4
-
L-Phe at pH 7.8 and 50°C Cerastes cerastes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Cerastes cerastes

Cofactor

Cofactor Comment Organism Structure
FAD
-
Cerastes cerastes

pI Value

Organism Comment pI Value Maximum pI Value
Cerastes cerastes isoelectric focusing
-
6.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
97.6
-
L-Leu at pH 7.8 and 50°C Cerastes cerastes
185
-
L-Met at pH 7.8 and 50°C Cerastes cerastes
392.5
-
L-Phe at pH 7.8 and 50°C Cerastes cerastes