Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetyl-L-tryptophan | about 90% inhibition at 50 nM | Cerastes cerastes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-Phe | at pH 7.8 and 50°C | Cerastes cerastes | |
0.1 | - |
L-Met | at pH 7.8 and 50°C | Cerastes cerastes | |
0.25 | - |
L-Leu | at pH 7.8 and 50°C | Cerastes cerastes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Cerastes cerastes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerastes cerastes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cerastes cerastes |
Purification (Comment) | Organism |
---|---|
Resource Q column chromatography, HiTrap Heparin column chromatograph, yand Sephadex G-75 gel filtration | Cerastes cerastes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Cerastes cerastes | - |
Storage Stability | Organism |
---|---|
-20°C, 24 h, 85% loss of activity | Cerastes cerastes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu + H2O + O2 | - |
Cerastes cerastes | 2-oxo-4-methylvaleric acid + NH3 + H2O2 | - |
? | |
L-Met + H2O + O2 | - |
Cerastes cerastes | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-Phe + H2O + O2 | - |
Cerastes cerastes | phenylpyruvate + NH3 + H2O2 | - |
? | |
additional information | casein is not a substrate | Cerastes cerastes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 58000, SDS-PAGE | Cerastes cerastes |
homodimer | 2 * 56573, calculated from amino acid sequence | Cerastes cerastes |
Synonyms | Comment | Organism |
---|---|---|
L-amino acid: O2 oxidoreductase | - |
Cerastes cerastes |
LAAO | - |
Cerastes cerastes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cerastes cerastes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | after 120 min incubation, the enzyme retains 80% of its activity at 50°C. At a temperature over 55°C, the activity decreases dramatically to reach 10% at 60°C | Cerastes cerastes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.5 | - |
L-Met | at pH 7.8 and 50°C | Cerastes cerastes | |
24.4 | - |
L-Leu | at pH 7.8 and 50°C | Cerastes cerastes | |
31.4 | - |
L-Phe | at pH 7.8 and 50°C | Cerastes cerastes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Cerastes cerastes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Cerastes cerastes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cerastes cerastes | isoelectric focusing | - |
6.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
97.6 | - |
L-Leu | at pH 7.8 and 50°C | Cerastes cerastes | |
185 | - |
L-Met | at pH 7.8 and 50°C | Cerastes cerastes | |
392.5 | - |
L-Phe | at pH 7.8 and 50°C | Cerastes cerastes |