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Literature summary for 1.4.3.2 extracted from

  • Kommoju, P.R.; Macheroux, P.; Ghisla, S.
    Molecular cloning, expression and purification of L-amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma (2007), Protein Expr. Purif., 52, 89-95.
    View publication on PubMed
Search for more literature for 1.4.3.2

Application

Application Comment Organism
additional information expressed LAAO exhibits the same electrophoretic mobility as native LAAO and exhibits approximately the same extent of glycosylation as authentic LAAO from snake venom. Catalytic properties and substrate specificity of recombinant LAAO are similar to those of native enzyme Calloselasma rhodostoma

Cloned(Commentary)

Cloned (Comment) Organism
LAAO open reading frame inserted after the alpha-MF-signal sequence. Expressed in Pichia pastoris Calloselasma rhodostoma

Inhibitors

Inhibitors Comment Organism Structure
tunicamycin inhibits secretion of recombinant LAAO Calloselasma rhodostoma

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Calloselasma rhodostoma
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Calloselasma rhodostoma
62000
-
 x * 62000, SDS-PAGE Calloselasma rhodostoma

Organism

Organism UniProt Comment Textmining
Calloselasma rhodostoma
-
-
-
Calloselasma rhodostoma
-
 expression in Pichia pastoris
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Calloselasma rhodostoma

Purification (Commentary)

Purification (Comment) Organism
by ion exchange and gel filtration, to homogeneity Calloselasma rhodostoma
recombinant enzyme Calloselasma rhodostoma

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 supernatant from the culture medium Calloselasma rhodostoma
3.15
-
 purified enzyme Calloselasma rhodostoma

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan Calloselasma rhodostoma 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-methionine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan Calloselasma rhodostoma 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-phenylalanine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan Calloselasma rhodostoma phenylpyruvate + NH3 + H2O2
-
 ?
L-tryptophan + H2O + O2 substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan Calloselasma rhodostoma 3-indole-2-oxopropanoate + NH3 + H2O2
-
 ?
L-tyrosine + H2O + O2 substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan Calloselasma rhodostoma p-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Calloselasma rhodostoma
homodimer 2 * 60000, SDS-PAGE Calloselasma rhodostoma

Synonyms

Synonyms Comment Organism
LAAO
-
 Calloselasma rhodostoma