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Literature summary for 1.4.3.2 extracted from

  • Costal-Oliveira, F.; Stransky, S.; Guerra-Duarte, C.; Naves de Souza, D.L.; Vivas-Ruiz, D.E.; Yarleque, A.; Sanchez, E.F.; Chavez-Olortegui, C.; Braga, V.M.M.
    L-amino acid oxidase from Bothrops atrox snake venom triggers autophagy, apoptosis and necrosis in normal human keratinocytes (2019), Sci. Rep., 9, 781 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
medicine LAAO is involved in dermonecrosis in mice and shows cytotoxic effects in normal human keratinocytes, the major cell type in the epidermis, which undergoes extensive necrosis at the snakebite site. Pharmacological inhibition by N-acetyl cysteine prevents Bothrops atrox venom-induced necrosis. Treatment with purified LAAO decreases keratinocyte viability with an effective Concentration (EC50) of 5.1 microg/ml. Cytotoxicity caused by LAAO is mediated by H2O2 and treated cells undergo autophagy, followed by apoptosis and necrosis. LAAO induces morphological alterations that precede cell death Bothrops atrox

Organism

Organism UniProt Comment Textmining
Bothrops atrox P0CC17
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Purification (Commentary)

Purification (Comment) Organism
purification by molecular exclusion, ion exchange and affinity purification Bothrops atrox

Source Tissue

Source Tissue Comment Organism Textmining
venom
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 Bothrops atrox
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Storage Stability

Storage Stability Organism
LAAO is more stable when kept at -80°C, followed by 4°C, reactivation using sodium acetate buffer pH 5.0 for 30 min at 37°C leads to increased activity Bothrops atrox

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE and mass spectrometry Bothrops atrox