KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
L-arginine | - |
Bacillus carotarum | |
0.259 | - |
L-asparagine | - |
Bacillus carotarum | |
0.34 | - |
L-glutamine | - |
Bacillus carotarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Bacillus carotarum |
102000 | 115000 | gel filtration, native PAGE | Bacillus carotarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus carotarum | - |
2Pfa | - |
Bacillus carotarum 2Pfa | - |
2Pfa | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus carotarum |
Storage Stability | Organism |
---|---|
-20°C, 4 months, no loss of activity | Bacillus carotarum |
4°C, 4 months, no loss of activity | Bacillus carotarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid + H2O + O2 | - |
Bacillus carotarum | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | - |
Bacillus carotarum 2Pfa | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-arginine + H2O + O2 | - |
Bacillus carotarum | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-arginine + H2O + O2 | - |
Bacillus carotarum 2Pfa | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-asparagine + H2O + O2 | - |
Bacillus carotarum | alpha-ketosuccinamic acid + NH3 + H2O2 | - |
r | |
L-glutamine + H2O + O2 | - |
Bacillus carotarum | 2-oxoglutaramate + NH3 + H2O2 | - |
r | |
L-leucine + H2O + O2 | - |
Bacillus carotarum | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | - |
Bacillus carotarum 2Pfa | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-lysine + H2O + O2 | - |
Bacillus carotarum | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | - |
Bacillus carotarum | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | - |
Bacillus carotarum 2Pfa | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | - |
Bacillus carotarum | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | - |
Bacillus carotarum 2Pfa | phenylpyruvate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Bacillus carotarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus carotarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | L-phenylalanine | Bacillus carotarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | - |
Bacillus carotarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol of FAD per mol of enzyme | Bacillus carotarum |