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Literature summary for 1.4.3.2 extracted from

  • Brearley, G.M.; Price, C.P.; Atkinson, T.; Hammond, P.M.
    Purification and partial characterization of a broad-range L-amino acid oxidase from Bacillus carotarum 2Pfa isolated from soil (1994), Appl. Microbiol. Biotechnol., 41, 670-676.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
L-arginine
-
Bacillus carotarum
0.259
-
L-asparagine
-
Bacillus carotarum
0.34
-
L-glutamine
-
Bacillus carotarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Bacillus carotarum
102000 115000 gel filtration, native PAGE Bacillus carotarum

Organism

Organism UniProt Comment Textmining
Bacillus carotarum
-
2Pfa
-
Bacillus carotarum 2Pfa
-
2Pfa
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus carotarum

Storage Stability

Storage Stability Organism
-20°C, 4 months, no loss of activity Bacillus carotarum
4°C, 4 months, no loss of activity Bacillus carotarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-amino acid + H2O + O2
-
Bacillus carotarum 2-oxo acid + NH3 + H2O2
-
?
L-amino acid + H2O + O2
-
Bacillus carotarum 2Pfa 2-oxo acid + NH3 + H2O2
-
?
L-arginine + H2O + O2
-
Bacillus carotarum 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
L-arginine + H2O + O2
-
Bacillus carotarum 2Pfa 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
L-asparagine + H2O + O2
-
Bacillus carotarum alpha-ketosuccinamic acid + NH3 + H2O2
-
r
L-glutamine + H2O + O2
-
Bacillus carotarum 2-oxoglutaramate + NH3 + H2O2
-
r
L-leucine + H2O + O2
-
Bacillus carotarum 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-leucine + H2O + O2
-
Bacillus carotarum 2Pfa 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-lysine + H2O + O2
-
Bacillus carotarum 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2
-
Bacillus carotarum 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-methionine + H2O + O2
-
Bacillus carotarum 2Pfa 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2
-
Bacillus carotarum phenylpyruvate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2
-
Bacillus carotarum 2Pfa phenylpyruvate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Bacillus carotarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus carotarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 L-phenylalanine Bacillus carotarum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5
-
Bacillus carotarum

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol of FAD per mol of enzyme Bacillus carotarum