Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.2 extracted from

  • Isobe, K.; Taira, R.; Hoshi, Y.; Matsuda, S.; Yamada, M.; Hibi, M.; Kishino, S.; Ogawa, J.
    Characterization of a novel L-amino acid oxidase with protein oxidizing activity from Penicillium steckii AIU 027 (2014), J. Biosci. Bioeng., 117, 690-695 .
    View publication on PubMed
Search for more literature for 1.4.3.2

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 2% residual activity at 1 mM Penicillium steckii
alpha,alpha'-dipyridyl 15% residual activity at 1 mM Penicillium steckii
Co2+ 88% residual activity at 1 mM Penicillium steckii
EDTA 86% residual activity at 1 mM Penicillium steckii
Fe3+ complete inhibition at 1 mM Penicillium steckii
hydrazine 93% residual activity at 1 mM Penicillium steckii
hydroxylamine 12% residual activity at 1 mM Penicillium steckii
iodoacetate 75% residual activity at 1 mM Penicillium steckii
KCN 83% residual activity at 1 mM Penicillium steckii
Mg2+ 91% residual activity at 1 mM Penicillium steckii
Mn2+ 92% residual activity at 1 mM Penicillium steckii
N-ethylmaleimide 87% residual activity at 1 mM Penicillium steckii
NaN3 91% residual activity at 1 mM Penicillium steckii
Ni2+ 90% residual activity at 1 mM Penicillium steckii
o-phenanthroline 84% residual activity at 1 mM Penicillium steckii
Semicarbazide 70% residual activity at 1 mM Penicillium steckii
Zn2+ 55% residual activity at 1 mM Penicillium steckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
 fish collagen peptide II at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.007
-
 Nepsilon-benzyloxycarbonyl-L-lysine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.0081
-
 fish collagen peptide I at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.01
-
 L-Lys at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.012
-
 salmon gelatin at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.017
-
 L-Orn at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.17
-
 beta-Lactoglobulin at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
0.25
-
 poly-L-lysine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
17
-
 Gly-L-Lys at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
26.5
-
 Nalpha-benzyloxycarbonyl-L-lysine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
37.7
-
 L-Ala-L-Lys at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
56.4
-
 Nalpha-benzyloxycarbonyl-L-ornithine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe the enzyme contains iron Penicillium steckii
additional information not influenced by Cu2+ Penicillium steckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
290000
-
 gel filtration Penicillium steckii

Organism

Organism UniProt Comment Textmining
Penicillium steckii
-
-
-
Penicillium steckii AIU 027
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Toyopearl column chromatography, DEAE Toyopearl column chromatography, aminooctyl Toyopearl column chromatography and hydroxyapatite column chromatography Penicillium steckii

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Penicillium steckii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-lactoglobulin + H2O + O2
-
 Penicillium steckii ?
-
 ?
fish collagen peptide I + H2O + O2
-
 Penicillium steckii ?
-
 ?
fish collagen peptide II + H2O + O2
-
 Penicillium steckii ?
-
 ?
Gly-L-Lys + H2O + O2
-
 Penicillium steckii ?
-
 ?
L-Ala-L-Lys + H2O + O2
-
 Penicillium steckii ?
-
 ?
L-Arg + H2O + O2
-
 Penicillium steckii 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
L-Arg + H2O + O2
-
 Penicillium steckii AIU 027 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
L-Glu + H2O + O2
-
 Penicillium steckii 2-oxoglutarate + NH3 + H2O2
-
 ?
L-Glu + H2O + O2
-
 Penicillium steckii AIU 027 2-oxoglutarate + NH3 + H2O2
-
 ?
L-Leu + H2O + O2
-
 Penicillium steckii 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
L-Lys + H2O + O2
-
 Penicillium steckii 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
L-Met + H2O + O2
-
 Penicillium steckii 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-Met + H2O + O2
-
 Penicillium steckii AIU 027 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-Orn + H2O + O2
-
 Penicillium steckii 2-oxo-5-aminopentanoate + NH3 + H2O2
-
 ?
L-Orn + H2O + O2
-
 Penicillium steckii AIU 027 2-oxo-5-aminopentanoate + NH3 + H2O2
-
 ?
additional information no activity with D-amino acids, aminoalcohols and amines as well as L-threonine, L-isoleucine, L-serine, L-valine, Lcysteine, L-aspartic acid, L-proline and glycine Penicillium steckii ?
-
 ?
additional information no activity with D-amino acids, aminoalcohols and amines as well as L-threonine, L-isoleucine, L-serine, L-valine, Lcysteine, L-aspartic acid, L-proline and glycine Penicillium steckii AIU 027 ?
-
 ?
myoglobin + H2O + O2
-
 Penicillium steckii ?
-
 ?
Nalpha-benzyloxycarbonyl-L-lysine + H2O + O2
-
 Penicillium steckii Nalpha-benzyloxycarbonyl-L-aminoadipic delta-semialdehyde + NH3 + H2O2
-
 ?
Nalpha-benzyloxycarbonyl-L-ornithine + H2O + O2
-
 Penicillium steckii ?
-
 ?
Nepsilon-benzyloxycarbonyl-L-lysine + H2O + O2
-
 Penicillium steckii ?
-
 ?
poly-L-lysine + H2O + O2
-
 Penicillium steckii ?
-
 ?
salmon gelatin + H2O + O2
-
 Penicillium steckii ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 75300, SDS-PAGE Penicillium steckii

Synonyms

Synonyms Comment Organism
L-AAO
-
 Penicillium steckii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 when the enzyme is incubated at pH 6.0 for 30 min at various temperatures, the enzyme is stable blow 40°C Penicillium steckii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 the enzyme is stable for 30 min in the pH range from 5.0 to 6.0, but more than 90% of original activity is lost in the alkaline region from pH 7.0 Penicillium steckii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Penicillium steckii

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium steckii isoelectric focusing
-
 3.2