Application | Comment | Organism |
---|---|---|
synthesis | engineering Escherichia coli to produce phenylpyruvate. Knock-out of three aminotransferases increases the phenylpyuvate titer from 3.3 to 3.9 g/l and the substrate conversion ratio to 97.5%. The L-amino acid deaminase triple mutant D165K/F263M/L336M produces 10.0 g phenylpyruvate per l, with a substrate conversion ratio of 100%. An optimal fed-batch biotransformation process gives 21 g phenylpyruvate per l within 8 h | Proteus mirabilis |
Cloned (Comment) | Organism |
---|---|
xcpression in Escherichia coli | Proteus mirabilis |
Protein Variants | Comment | Organism |
---|---|---|
D165K | slight increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
D165K/F263M | slight increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
D165K/F263M/L336 | 2fold increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
D165K/L336M | slight increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
D165K/S179L/F263V/L336V | slight increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
F263M | slight increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
F263M/L336M | 2fold increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
L336M | slight increase in kcat/KM value for phenylpyruvate | Proteus mirabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
L-phenylalanine | mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
22.4 | - |
L-phenylalanine | mutant F263M/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
22.6 | - |
L-phenylalanine | mutant D165K/F263M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
23.8 | - |
L-phenylalanine | mutant F263M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
24.1 | - |
L-phenylalanine | mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
24.3 | - |
L-phenylalanine | mutant D165K/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
24.5 | - |
L-phenylalanine | mutant L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
24.8 | - |
L-phenylalanine | mutant D165K, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
26.2 | - |
L-phenylalanine | wild-type, pH 7.0, temperature not specified in the publication | Proteus mirabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus mirabilis | - |
- |
- |
Proteus mirabilis KCTC 2566 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + O2 | - |
Proteus mirabilis | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | - |
Proteus mirabilis KCTC 2566 | phenylpyruvate + NH3 + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
L-phenylalanine | wild-type, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
1.67 | - |
L-phenylalanine | mutant D165K, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
1.69 | - |
L-phenylalanine | mutant L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
1.72 | - |
L-phenylalanine | mutant D165K/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
1.82 | - |
L-phenylalanine | mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
1.87 | - |
L-phenylalanine | mutant F263M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
2.16 | - |
L-phenylalanine | mutant D165K/F263M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
2.21 | - |
L-phenylalanine | mutant F263M/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
2.25 | - |
L-phenylalanine | mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
L-phenylalanine | wild-type, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.067 | - |
L-phenylalanine | mutant D165K, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.069 | - |
L-phenylalanine | mutant L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.071 | - |
L-phenylalanine | mutant D165K/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.076 | - |
L-phenylalanine | mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.079 | - |
L-phenylalanine | mutant F263M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.096 | - |
L-phenylalanine | mutant D165K/F263M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.099 | - |
L-phenylalanine | mutant F263M/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis | |
0.102 | - |
L-phenylalanine | mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication | Proteus mirabilis |