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3.4.21.26: prolyl oligopeptidase

This is an abbreviated version!
For detailed information about prolyl oligopeptidase, go to the full flat file.

Word Map on EC 3.4.21.26

Reaction

Hydrolysis of --Pro-/- and to a lesser extent --Ala-/- in oligopeptides =

Synonyms

apPEP, cyproase I, EC 3.4.22.18, endoprolylpeptidase, eryngase, FAP, fibroblast activation protein, FlaP, glutenase, membrane-bound PE, More, mPOP, PE, PEP, PepO2, peptidase, postproline endo-, POP, POP Tb, POP Tc80, POPA, POPB, post proline cleaving enzyme, post prolyl cleaving enzyme, post-proline cleaving endopeptidase, post-proline cleaving enzyme, post-proline cutting enzyme, post-proline endopeptidase, postproline cleaving enzyme, postproline endopeptidase, postproline-cleaving enzyme, PPCE, PREP, PREPL A, proline endopeptidase, proline specific prolyl endopeptidase, proline-specific endopeptidase, proly endopeptidase, prolyl endopeptidase, prolyl endoprotease, prolyl oligopeptidase, prolyl oligopeptidase B, prolylendopeptidase, prost-proline cleaving enzyme, PsE, S28A, S28B, TNA1_POP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.26 prolyl oligopeptidase

Purification

Purification on EC 3.4.21.26 - prolyl oligopeptidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1.47fold after liquid chromatography, ionex column chromatography, gel chromatography
affinity chromatography
affinity purification
-
ammonium sulfate precipitation and Butyl Toyopearl column chromatography
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ammonium sulfate precipitation and Ni-Sepharose resin column chromatography
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ammonium sulfate precipitation DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography
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ammonium sulfate precipitation, DEAE-Sephacel column chromatography, phenyl Sepharose column chromatography, and Q-Sepharose column chromatography
-
ammonium sulfate precipitation, HiTrap DEAE column chromatography, and HiTrap Q column chromatography
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, hydroxyapatite column chromatography, and Resource Q column chromatography
-
anion exchange chromatography
ethanol precipitation, Fractogel EMD SO3– chromatography, Blue Sepharose column chromatography, and HA Ultrogel chromatography
-
extracellular isozyme, cytsolic isozyme, overview
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glutathione Sepharose column chromatography and Mono Q 5/50 GL column chromatography
-
gluthathione Sepharose column chromatography and Mono Q column chromatography
-
Hi-Trap column chromatography and Mono Q column chromatography
HisTrap column chromatography, HiTrap Q column chromatography, and Superdex S200 gel filtration
-
HiTrap DEAE column chromatography
-
Mono Q column chromatography
more than 90% purity
-
native enzyme expressed in Escherichia coli is rapidly thermally inactivated. Mutation E289G improves protein stability
-
Ni-NTA affinity column chromatography and Ultragel AcA 44 gel filtration
-
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography
Ni2+ affinity column chromatography and Superdex S200 gel filtration
-
nickel affinity chromatography, thrombin cleavage, gel filtration and concentrating with a Millipore Centripep YM10 filtration unit
partial
partial, ion exchange chromatography
-
Q-Sepharose column chromatography, Cu2+ Sepharose column chromatography, and Sephacryl S-100 gel filtration
-
recombinant enzyme from Escherichia coli by two different steps of anion exchange chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and anion exchange chromatography
recombinant tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography
wild-type and recombinant enzyme
-