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Literature summary for 3.4.21.26 extracted from

  • Li, M.; Chen, C.; Davies, D.R.; Chiu, T.K.
    An induced-fit mechanism for prolyl endopeptidase (2010), J. Biol. Chem., 285, 21487-95.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Aeromonas caviae

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Aeromonas caviae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.81
-
 Z-Gly-Pro-4-nitroanilide
-
 Aeromonas caviae

Organism

Organism UniProt Comment Textmining
Aeromonas caviae Q9X6R4
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography, thrombin cleavage, gel filtration and concentrating with a Millipore Centripep YM10 filtration unit Aeromonas caviae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Gly-Pro-4-nitroanilide + H2O assay at pH 8.0, 34°C Aeromonas caviae Z-Gly-Pro + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
apPEP
-
 Aeromonas caviae
prolyl endopeptidase
-
 Aeromonas caviae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
 assay at Aeromonas caviae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
505
-
 Z-Gly-Pro-4-nitroanilide
-
 Aeromonas caviae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Aeromonas caviae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
623
-
 Z-Gly-Pro-4-nitroanilide
-
 Aeromonas caviae