Literature summary for 3.4.21.26 extracted from

Huang, C.H.; Suen, C.S.; Lin, C.T.; Chien, C.H.; Lee, H.Y.; Chung, K.M.; Tsai, T.Y.; Jiaang, W.T.; Hwang, M.J.; Chen, X.
Cleavage-site specificity of prolyl endopeptidase FAP investigated with a full-length protein substrate (2011), J. Biochem., 149, 685-692.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Hi5 insect cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
(2S)-1-({(2S,4S)-4-[2-(1,3-Dihydro-2H-isoindol-2-yl)-2-oxo- ethyl]-5-oxopyrrolidin-2-yl}carbonyl)-4,4-difluoropyrrolidine-2- carbonitrile	potent and selective inhibitor of FAP, 0.01 mM used in assay conditions	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpa2-antiplasmin + H2O	Homo sapiens	not a robust substrate in vitro, the enzyme cleaves after Pro12 in the T9S10G11P12-N13 Q14E15Q16E17 sequence	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Ni-Sepharose resin column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpa2-antiplasmin + H2O	not a robust substrate in vitro, the enzyme cleaves after Pro12 in the T9S10G11P12-N13 Q14E15Q16E17 sequence	Homo sapiens	?	-	?
additional information	fibroblast activation protein prefers uncharged residues, including small or bulky hydrophobic amino acids, but not charged amino acids, especially acidic residue at P1, P3 and P4 sites. Fibroblast activation protein cannot cleave interleukins	Homo sapiens	?	-	?
SPRY2 + H2O	not an in vivo substrate of fibroblast activation protein	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
FAP	-	Homo sapiens
fibroblast activation protein	-	Homo sapiens
prolyl endopeptidase	-	Homo sapiens

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Release 2023.1 (January 2023)