3.4.21.26: prolyl oligopeptidase

This is an abbreviated version!
For detailed information about prolyl oligopeptidase, go to the full flat file.

Reaction

Hydrolysis of --Pro-/- and to a lesser extent --Ala-/- in oligopeptides =

Synonyms

apPEP, cyproase I, EC 3.4.22.18, endoprolylpeptidase, eryngase, FAP, fibroblast activation protein, FlaP, glutenase, membrane-bound PE, More, mPOP, PE, PEP, PepO2, peptidase, postproline endo-, POP, POP Tb, POP Tc80, POPA, POPB, post proline cleaving enzyme, post prolyl cleaving enzyme, post-proline cleaving endopeptidase, post-proline cleaving enzyme, post-proline cutting enzyme, post-proline endopeptidase, postproline cleaving enzyme, postproline endopeptidase, postproline-cleaving enzyme, PPCE, PREP, PREPL A, proline endopeptidase, proline specific prolyl endopeptidase, proline-specific endopeptidase, proly endopeptidase, prolyl endopeptidase, prolyl endoprotease, prolyl oligopeptidase, prolyl oligopeptidase B, prolylendopeptidase, prost-proline cleaving enzyme, PsE, S28A, S28B, TNA1_POP

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.26 prolyl oligopeptidase

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Results	in table
15	Activating Compound
1921	AA Sequence
28	Application
1	CAS Registry Number
40	Cloned(Commentary)
12	Crystallization (Commentary)
1563	Disease/ Diagnostics
8	Expression
34	General Information
6	General Stability
294	IC50 Value
923	Inhibitors
45	kcat/KM [mM/s]
96	Ki Value [mM]
178	KM Value [mM]
86	Localization
17	Metals/Ions
88	Molecular Weight [Da]
234	Natural Substrates/ Products (Substrates)
249	Organism
2	Oxidation Stability
38	PDB ID
82	pH Optimum
20	pH Range
12	pH Stability
18	pI Value
7	Posttranslational Modification
55	Protein Variants
58	Purification (Commentary)
27	Reaction
12	Reaction Type
249	Reference
1	Renatured (Commentary)
386	Source Tissue
77	Specific Activity [micromol/min/mg]
3	Storage Stability
733	Substrates and Products (Substrate)
65	Subunits
285	Synonyms
44	Temperature Optimum [°C]
3	Temperature Range [°C]
21	Temperature Stability [°C]
155	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 3.4.21.26 - prolyl oligopeptidase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

4.08

Ala-Pro-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

1.31 - 106

Arg-Pro-4-nitroanilide

2 entries

1.82

Asp-Pro-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

57.6

benzyloxycarbonyl-Ala-Ala-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

410

benzyloxycarbonyl-Ala-Pro-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

47.4

benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide

Coprinopsis clastophylla

-

at pH 8.0 and 37°C

732333

15.1

benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin

Coprinopsis clastophylla

-

at pH 8.0 and 37°C

732333

0.16 - 6.9

calcitonin gene-related peptide

6 entries

-

2.9

Gly-Pro-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

496.4 - 596.7

L-Ala-L-Ala-L-Pro-4-nitroanilide

2 entries

5.7 - 4900

L-Leu-4-nitroanilide

10 entries

700 - 30000

L-Phe-4-nitroanilide

10 entries

33 - 209

N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin

2 entries

24 - 285.7

Z-Gly-L-Pro-4-nitroanilide

2 entries

623

Z-Gly-Pro-4-nitroanilide

Aeromonas caviae

Q9X6R4

-

709120

166.1 - 202.7

Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide

2 entries

additional information

additional information

Pyrococcus furiosus

-

kinetic and mechanistic studies

722617

-

1.31

Arg-Pro-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

106

Arg-Pro-4-nitroanilide

Pyrococcus furiosus

-

pH 7.5, 85°C

722617

0.16

calcitonin gene-related peptide

Mus musculus

-

wild type enzyme, diserine mutant of calcitonin gene-related peptide

707485

-

0.22

calcitonin gene-related peptide

Mus musculus

-

mutant D35A/K196A, full length calcitonin gene-related peptide

707485

-

0.28

calcitonin gene-related peptide

Mus musculus

-

wild type enzyme, full length calcitonin gene-related peptide

707485

-

0.66

calcitonin gene-related peptide

Mus musculus

-

mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide

707485

-

4.3

calcitonin gene-related peptide

Mus musculus

-

mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide

707485

-

6.9

calcitonin gene-related peptide

Mus musculus

-

wild type enzyme, shorter peptide of calcitonin gene-related peptide

707485

-

496.4

L-Ala-L-Ala-L-Pro-4-nitroanilide

Aspergillus oryzae

-

at 40°C and pH 4.0

732282

596.7

L-Ala-L-Ala-L-Pro-4-nitroanilide

Aspergillus oryzae

-

at pH 5.0 and 35°C

732281

5.7

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570K, at pH 8.0 and 25°C

753033

8.9

L-Leu-4-nitroanilide

Pleurotus eryngii

-

oxidized wild type enzyme, at pH 8.0 and 25°C

753033

35

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570F, at pH 8.0 and 25°C

753033

94

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M203Q/M570Q, at pH 8.0 and 25°C

753033

110

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M142Q/M203Q/M570Q/M620Q, at pH 8.0 and 25°C

753033

200

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570Q, at pH 8.0 and 25°C

753033

220

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570A, at pH 8.0 and 25°C

753033

220

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570E, at pH 8.0 and 25°C

753033

720

L-Leu-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M203Q, at pH 8.0 and 25°C

753033

4900

L-Leu-4-nitroanilide

Pleurotus eryngii

-

wild type enzyme, at pH 8.0 and 25°C

753033

700

L-Phe-4-nitroanilide

Pleurotus eryngii

-

oxidized wild type enzyme, at pH 8.0 and 25°C

753033

1100

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570K, at pH 8.0 and 25°C

753033

1800

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570F, at pH 8.0 and 25°C

753033

3500

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M203Q/M570Q, at pH 8.0 and 25°C

753033

3900

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570Q, at pH 8.0 and 25°C

753033

4600

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M142Q/M203Q/M570Q/M620Q, at pH 8.0 and 25°C

753033

6800

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570A, at pH 8.0 and 25°C

753033

8300

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M570E, at pH 8.0 and 25°C

753033

10000

L-Phe-4-nitroanilide

Pleurotus eryngii

-

mutant enzyme M203Q, at pH 8.0 and 25°C

753033

30000

L-Phe-4-nitroanilide

Pleurotus eryngii

-

wild type enzyme, at pH 8.0 and 25°C

753033

33

N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin

Trypanosoma brucei brucei

Q6HA27

in absence of DTT

709834

209

N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin

Trypanosoma brucei brucei

Q6HA27

in presence of DTT

709834

24

Z-Gly-L-Pro-4-nitroanilide

Aspergillus oryzae

-

at pH 5.0 and 35°C

732281

285.7

Z-Gly-L-Pro-4-nitroanilide

Aspergillus oryzae

-

at 40°C and pH 4.0

732282

166.1

Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide

Aspergillus oryzae

-

at 40°C and pH 4.0

732282

202.7

Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide

Aspergillus oryzae

-

at pH 5.0 and 35°C

732281