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3.4.11.2: membrane alanyl aminopeptidase

This is an abbreviated version!
For detailed information about membrane alanyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.2

Reaction

Release of an N-terminal amino acid, Xaa-/-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide =

Synonyms

AAP, Aap 1' aminopeptidase, Aap1 aminopeptidase, Aap1', AlaAP, alanine aminopeptidase, alanine-specific aminopeptidase, alanine/arginine aminopeptidase, alanyl aminopeptidase, alanyl-aminopeptidase, Alpha-aminoacylpeptide hydrolase, amino-oligopeptidase, amino-peptidase N, aminopeptidase D, aminopeptidase M, aminopeptidase M II, aminopeptidase N, aminopeptidase N type 1, aminopeptidase N/CD13, aminopeptidase N3, aminopeptidase, microsomal, aminopeptidase-N, ANPEP, AP-N, Ap1, Ap2, APAse D, APase2, Ape, APN, APN-1, APN-PI, APN/CD13, APN1, APN2, APN3, APN96, basic aminopeptidase, beta-alanyl aminopeptidase, CD13, Cl--activated aminopeptidase, CryIA(C) receptor, Cys-Gly-dipeptidase, cysteinyl-glycinase, dipeptidase, DP, EC 3.4.1. 2, EC 3.4.1.2, EC 3.4.3.5, ENPEP, ePepN, extracellular aminopeptidase, GP 130, GP150, L,(L/D)-aminopeptidase, L-alanine aminopeptidase, LAP, lap3, LeuAP M, leucine aminopeptidase, Leukemia antigen CD13, Lys-AP, Lysyl aminopeptidase, M1 alanine aminopeptidase, mAAP, membrane alanyl aminopeptidase N, membrane alanyl-aminopeptidase, Membrane glycoprotein H11, Membrane protein p161, Microsomal aminopeptidase, microsomal leucine aminopeptidase, More, Myeloid plasma membrane glycoprotein CD13, neutral aminopeptidase, NmAPN, p146, p146 type II alveolar epithelial cell antigen, p161, particle-bound aminopeptidase, PepN, peptidase N, plant aminopeptidase N, puromycin-insensitive neutral aminopeptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.2 membrane alanyl aminopeptidase

Cloned

Cloned on EC 3.4.11.2 - membrane alanyl aminopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 70 kDa fragment of the 106 kDa APN is expressed in Escherichia coli BL21-CodonPlus (DE3)/pRIL cells
by RT-PCR, DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue
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DNA and amino acid sequence determination and analysis, genomic organization and structure, phylogenetic and evolutionary analysis
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DNA and amino acid sequence determination and analysis, identification of six polymorphisms e.g. C956T, G978T, G987A, and C398T, allele frequency and haplotype identification, overview
DNA and amino acid sequence determination and analysis, quantitativ expression analysis in monocytes and macrophages of percardial fluid
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expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
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expressed in Escherichia coli DH10B cells (binds Cry11Aa toxin with high affinity) and in Sf21 cells (enzymatically active, has a GPI-anchor but does not bind Cry11Aa toxin)
expressed in Escherichia coli DH5alpha and HEK-293T cells cells
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expression analysis
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expression in Escherichia coli
expression in Escherichia coli, which is more difficult for the full-length enzyme as for fragments, successfull expression of isozyme APN1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, but not of isozyme APN2, transient expression of isozyme APN1 on the cell surface in Sf21 cells under control of the inducible Drosophila HSP70 promoter
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expression in Escherichia coli, with His-tag
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expression in HEK-293 cell
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expression in HT-1080 cell
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expression in human embryonic kidney cell
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expression in LLC-PK1 cell
expression in Sf21 insect cells
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expression of the enzyme in murine CMT93 cells and in transgenic mice
expression of wild-type and mutant E350D in COS-7 cells
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four isozymes APN1-4, DNA and amino acid sequence determination and analysis
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from a kidney library
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from an intestinal cDNA library, the gene is localized on chromosome 15, intestinal and myeloid epithelial cells use different promoters
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gene AAP1', DNA and amino acid sequence determination and analysis, genetic structure, overview
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gene APN3harm, DNA and amino acid sequence determination and analysis from three Cry1Ac toxin-susceptible and three Cry1Ac toxin-resistant strains, expression of wild-type and mutant enzymes in Escherichia coli, the functional recombinant enzyme can bind the Cry1Ac toxin
gene mapping on chromosome 15q25-26, DNA and amino acid sequence analysis
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gene pepN, cloning from genomic DNA of strain W3110, overexpression in strain BL21(DE3), method optimization
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gene pepN, DNA and amino acid sequence determination and analysis, overvexpression
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isozyme BmAPN3, DNA and amino acid sequence determination and analysis
isozyme PxAPN3, DNA and amino acid sequence determination and analysis
overexpression in tumour cells
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recombinant expression of His-tagged enzyme APN in Spodoptera frugiperda SF9 cells using the baculovirus transfection system, recombinant expression of pea aphid APN in Escherichia coli for the production of polyclonal antibodies
the gene is localized on chromosome 7
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transient expression of wild-type and mutant enzymes in COS-1 cells, as GFP-tagged proteins, and in renal carcinoma N109 cells
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transient expression of wild-type and mutant enzymes in murine BALB/3T3 fibroblasts, and stable expression in CL1-0 cells
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