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Literature summary for 3.4.11.2 extracted from

  • Byun, T; Tang, M.; Sloma, A.; Brown, K.M.; Marumoto, C.; Fujii, M.; Blinkovsky, A.M.
    Aminopeptidase from Sphingomonas capsulata (2001), J. Biol. Chem., 276, 17902-17907.
    View publication on PubMed
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue Novosphingobium capsulatum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Novosphingobium capsulatum
additional information no inhibition by EDTA and PMSF Novosphingobium capsulatum
Zn2+ slight inhibition, as ferrocyanide, iodate, and phosphate, Ki value depends on solubility of the salt Novosphingobium capsulatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
 L-Ala-2-naphthylamide pH 7.5, 43°C Novosphingobium capsulatum
14
-
 L-Ala-4-nitroanilide pH 7.5, 43°C Novosphingobium capsulatum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Novosphingobium capsulatum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the enzyme contains the zinc binding motif HEXXH Novosphingobium capsulatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Novosphingobium capsulatum

Organism

Organism UniProt Comment Textmining
Novosphingobium capsulatum
-
-
-
Novosphingobium capsulatum IFO 12533
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture broth supernatant by ultrafiltration, ion exchange chromatography, and gel filtration Novosphingobium capsulatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
 purified enzyme Novosphingobium capsulatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-4-nitroanilide + H2O 14% of the activity with L-Ala-4-nitroanilide Novosphingobium capsulatum Gly + 4-nitroaniline
-
 ?
L-Ala-2-naphthylamide + H2O
-
 Novosphingobium capsulatum L-Ala + 2-naphthylamine
-
 ?
L-Ala-4-nitroanilide + H2O best substrate Novosphingobium capsulatum L-Ala + 4-nitroaniline
-
 ?
L-Asp-4-nitroanilide + H2O 14% of the activity with L-Ala-4-nitroanilide Novosphingobium capsulatum L-Asp + 4-nitroaniline
-
 ?
L-Glu-4-nitroanilide + H2O 12% of the activity with L-Ala-4-nitroanilide Novosphingobium capsulatum L-Glu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O 27% of the activity with L-Ala-4-nitroanilide Novosphingobium capsulatum L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O 3% of the activity with L-Ala-4-nitroanilide Novosphingobium capsulatum L-Lys + 4-nitroaniline
-
 ?
L-Met-4-nitroanilide + H2O 24% of the activity with L-Ala-4-nitroanilide Novosphingobium capsulatum L-Met + 4-nitroaniline
-
 ?
additional information no activity with 4-nitroanilides of Pro, Ile, Val, and Phe Novosphingobium capsulatum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Novosphingobium capsulatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
 Novosphingobium capsulatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 20 min, purified enzyme, loss of 40% activity Novosphingobium capsulatum
50
-
 20 min, purified enzyme, inactivation Novosphingobium capsulatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
860
-
 L-Ala-2-naphthylamide pH 7.5, 43°C Novosphingobium capsulatum
7600
-
 L-Ala-4-nitroanilide pH 7.5, 43°C Novosphingobium capsulatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Novosphingobium capsulatum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Novosphingobium capsulatum