Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.2 extracted from

  • Golich, F.C.; Han, M.; Crowder, M.W.
    Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli (2006), Protein Expr. Purif., 47, 634-639.
    View publication on PubMed
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
gene pepN, cloning from genomic DNA of strain W3110, overexpression in strain BL21(DE3), method optimization Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information poor or no inhibition by Fe2+ Escherichia coli
Zn2+ recombinant enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Escherichia coli
0.2
-
 L-Leu-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
0.28
-
 L-Arg-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
0.38
-
 L-Ala-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 5 mol per mol of enzyme Escherichia coli
Zn2+ 0.1 mol per mole of enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98750
-
 mass spectrometry Escherichia coli
99000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene pepN
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 3.8fold from overexpressing strain BL21(DE3) by anion exchange chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
964
-
 purified recombinant enzyme, substrate L-Ala-4-nitroanilide Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O
-
 Escherichia coli L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O
-
 Escherichia coli L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Escherichia coli L-Leu + 4-nitroaniline
-
 ?
additional information no activity with L-Val-4-nitroanilide, L-Met-4-nitroanilide, and L-Pro-4-nitroanilide Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 98750, recombinant enzyme, mass spectrometry Escherichia coli

Synonyms

Synonyms Comment Organism
aminopeptidase N
-
 Escherichia coli
PepN
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 L-Leu-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
158
-
 L-Arg-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli
354
-
 L-Ala-4-nitroanilide pH 7.0, 37°C, recombinant enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli