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Literature summary for 3.4.11.2 extracted from

  • Pillay, D.; Boulange, A.F.; Coustou, V.; Baltz, T.; Coetzer, T.H.
    Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense (2013), Exp. Parasitol., 135, 675-684.
    View publication on PubMed
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trypanosoma congolense

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Trypanosoma congolense
amastatin
-
 Trypanosoma congolense
bestatin
-
 Trypanosoma congolense
EDTA
-
 Trypanosoma congolense
puromycin
-
 Trypanosoma congolense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0329
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.04862
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.0579
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.08295
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.086
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.098
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.139
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
0.164
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Trypanosoma congolense 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, relative activity: 121%, compared to no cation: 100% Trypanosoma congolense
Ca2+ 10 mM, relative activity: 137%, compared to no cation: 100% Trypanosoma congolense
Co2+ 10 mM, relative activity: 151%, compared to no cation: 100% Trypanosoma congolense
Co2+ 10 mM, relative activity: 84%, compared to no cation: 100% Trypanosoma congolense
Cu2+ 10 mM, leads to complete inhibition Trypanosoma congolense
Cu2+ 10 mM, relative activity: 12%, compared to no cation: 100% Trypanosoma congolense
Fe2+ 10 mM, relative activity: 126%, compared to no cation: 100% Trypanosoma congolense
Fe2+ 10 mM, relative activity: 139%, compared to no cation: 100% Trypanosoma congolense
Mg2+ 10 mM, relative activity: 107%, compared to no cation: 100% Trypanosoma congolense
Mg2+ 10 mM, relative activity: 149%, compared to no cation: 100% Trypanosoma congolense
Mn2+ 10 mM, relative activity: 140%, compared to no cation: 100% Trypanosoma congolense
Mn2+ 10 mM, relative activity: 158%, compared to no cation: 100% Trypanosoma congolense
Ni2+ 10 mM, relative activity: 133%, compared to no cation: 100% Trypanosoma congolense
Ni2+ 10 mM, relative activity: 141%, compared to no cation: 100% Trypanosoma congolense
Zn2+ 10 mM, relative activity: 117%, compared to no cation: 100% Trypanosoma congolense
Zn2+ 10 mM, relative activity: 84%, compared to no cation: 100% Trypanosoma congolense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
 SDS-PAGE Trypanosoma congolense

Organism

Organism UniProt Comment Textmining
Trypanosoma congolense F9W8I6
-
-
Trypanosoma congolense G0UZY5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense Gly + 7-amino-4-methylcoumarin
-
 ?
L-Ala-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense L-Ala + 7-amino-4-methylcoumarin
-
 ?
L-Leu-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense L-Leu + 7-amino-4-methylcoumarin
-
 ?
L-Met-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense L-Met + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
alanyl aminopeptidase
-
 Trypanosoma congolense
Ap1
-
 Trypanosoma congolense
Ap2
-
 Trypanosoma congolense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma congolense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Trypanosoma congolense

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000014
-
 EDTA pH 8, 37°C Trypanosoma congolense
0.0000003
-
 EDTA pH 8, 37°C Trypanosoma congolense
0.0000024
-
 amastatin pH 8, 37°C Trypanosoma congolense
0.0000034
-
 bestatin pH 8, 37°C Trypanosoma congolense
0.0000086
-
 amastatin pH 8, 37°C Trypanosoma congolense
0.034
-
 1,10-phenanthroline pH 8, 37°C Trypanosoma congolense
0.048
-
 1,10-phenanthroline pH 8, 37°C Trypanosoma congolense
0.116
-
 puromycin pH 8, 37°C Trypanosoma congolense
0.447
-
 puromycin pH 8, 37°C Trypanosoma congolense

General Information

General Information Comment Organism
malfunction down-regulation by RNAi results in a slightly reduced growth rate in procyclic parasites in vitro Trypanosoma congolense

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.83
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
2.21
-
 L-Leu-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
48.96
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
69.92
-
 L-Met-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
466
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
686
-
 Gly-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
1807
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense
1853
-
 L-Ala-7-amido-4-methylcoumarin pH 8, 37°C Trypanosoma congolense