Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Trypanosoma congolense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Trypanosoma congolense | |
amastatin | - |
Trypanosoma congolense | |
bestatin | - |
Trypanosoma congolense | |
EDTA | - |
Trypanosoma congolense | |
puromycin | - |
Trypanosoma congolense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0329 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.04862 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.0579 | - |
Gly-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.08295 | - |
Gly-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.086 | - |
L-Met-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.098 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.139 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
0.164 | - |
L-Met-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Trypanosoma congolense | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, relative activity: 121%, compared to no cation: 100% | Trypanosoma congolense | |
Ca2+ | 10 mM, relative activity: 137%, compared to no cation: 100% | Trypanosoma congolense | |
Co2+ | 10 mM, relative activity: 151%, compared to no cation: 100% | Trypanosoma congolense | |
Co2+ | 10 mM, relative activity: 84%, compared to no cation: 100% | Trypanosoma congolense | |
Cu2+ | 10 mM, leads to complete inhibition | Trypanosoma congolense | |
Cu2+ | 10 mM, relative activity: 12%, compared to no cation: 100% | Trypanosoma congolense | |
Fe2+ | 10 mM, relative activity: 126%, compared to no cation: 100% | Trypanosoma congolense | |
Fe2+ | 10 mM, relative activity: 139%, compared to no cation: 100% | Trypanosoma congolense | |
Mg2+ | 10 mM, relative activity: 107%, compared to no cation: 100% | Trypanosoma congolense | |
Mg2+ | 10 mM, relative activity: 149%, compared to no cation: 100% | Trypanosoma congolense | |
Mn2+ | 10 mM, relative activity: 140%, compared to no cation: 100% | Trypanosoma congolense | |
Mn2+ | 10 mM, relative activity: 158%, compared to no cation: 100% | Trypanosoma congolense | |
Ni2+ | 10 mM, relative activity: 133%, compared to no cation: 100% | Trypanosoma congolense | |
Ni2+ | 10 mM, relative activity: 141%, compared to no cation: 100% | Trypanosoma congolense | |
Zn2+ | 10 mM, relative activity: 117%, compared to no cation: 100% | Trypanosoma congolense | |
Zn2+ | 10 mM, relative activity: 84%, compared to no cation: 100% | Trypanosoma congolense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
SDS-PAGE | Trypanosoma congolense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma congolense | F9W8I6 | - |
- |
Trypanosoma congolense | G0UZY5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | Gly + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
L-Met-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | L-Met + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alanyl aminopeptidase | - |
Trypanosoma congolense |
Ap1 | - |
Trypanosoma congolense |
Ap2 | - |
Trypanosoma congolense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma congolense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Trypanosoma congolense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000014 | - |
EDTA | pH 8, 37°C | Trypanosoma congolense | |
0.0000003 | - |
EDTA | pH 8, 37°C | Trypanosoma congolense | |
0.0000024 | - |
amastatin | pH 8, 37°C | Trypanosoma congolense | |
0.0000034 | - |
bestatin | pH 8, 37°C | Trypanosoma congolense | |
0.0000086 | - |
amastatin | pH 8, 37°C | Trypanosoma congolense | |
0.034 | - |
1,10-phenanthroline | pH 8, 37°C | Trypanosoma congolense | |
0.048 | - |
1,10-phenanthroline | pH 8, 37°C | Trypanosoma congolense | |
0.116 | - |
puromycin | pH 8, 37°C | Trypanosoma congolense | |
0.447 | - |
puromycin | pH 8, 37°C | Trypanosoma congolense |
General Information | Comment | Organism |
---|---|---|
malfunction | down-regulation by RNAi results in a slightly reduced growth rate in procyclic parasites in vitro | Trypanosoma congolense |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
2.21 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
48.96 | - |
L-Met-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
69.92 | - |
L-Met-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
466 | - |
Gly-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
686 | - |
Gly-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
1807 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense | |
1853 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8, 37°C | Trypanosoma congolense |