Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme might be a valuable histological marker for prostate cancer | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Rattus norvegicus |
from a kidney library | Oryctolagus cuniculus |
from an intestinal cDNA library, the gene is localized on chromosome 15, intestinal and myeloid epithelial cells use different promoters | Homo sapiens |
the gene is localized on chromosome 7 | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | IC50: 0.002 mM, specific inhibitor | Sus scrofa | |
amastatin | slow, tight-binding inhibitor which involves a conformational change in the enzyme-inhibitor complex | Sus scrofa | |
bestatin | - |
Sus scrofa | |
Metal chelating agents | - |
Sus scrofa | |
additional information | no inhibition by sulfhydryl reagents | Sus scrofa | |
probestin | IC50: 50 nM | Sus scrofa | |
puromycin | very weak inhibition | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | CD13 | Homo sapiens | 9986 | - |
membrane | - |
Lactococcus lactis | 16020 | - |
membrane | - |
Lymantria dispar | 16020 | - |
membrane | brush border membranes | Homo sapiens | 16020 | - |
membrane | brush border membranes | Rattus norvegicus | 16020 | - |
membrane | brush border membranes | Sus scrofa | 16020 | - |
membrane | brush border membranes | Oryctolagus cuniculus | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Sus scrofa | - |
- |
microsome | - |
Oryctolagus cuniculus | - |
- |
plasma membrane | type II integral membrane protein located on the plasma membrane as an ectoenzyme | Homo sapiens | 5886 | - |
plasma membrane | type II integral membrane protein located on the plasma membrane as an ectoenzyme | Rattus norvegicus | 5886 | - |
plasma membrane | type II integral membrane protein located on the plasma membrane as an ectoenzyme | Sus scrofa | 5886 | - |
plasma membrane | type II integral membrane protein located on the plasma membrane as an ectoenzyme | Oryctolagus cuniculus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains a typical zinc-binding motif HEXXH, one Zn2+ per subunit | Rattus norvegicus | |
Zn2+ | zinc-metallopeptidase, Zn2+ binds to the active site | Homo sapiens | |
Zn2+ | zinc-metallopeptidase, Zn2+ binds to the active site | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
kidney brush border enzyme, gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sus scrofa | the brain enzyme is involved in termination of the action of neuropeptides, especially of enkephalins and angiotensin III, the kidney enzyme contributes to the extracellular catabolism of glutathione, the intestinal enzyme acts as a receptor for the transmissible gastroenteritis virus, a coronavirus, causing fatal diarrhea in newborn pigs | ? | - |
? | |
additional information | Lymantria dispar | the enzyme acts as major receptor for the CryIAc toxin of Bacillus thuringiensis | ? | - |
? | |
additional information | Homo sapiens | the enzyme in granulocytes may cooperate with neprilysin, the enzyme is involved in the metabolism of certain vasoactive peptides in vascular cells in the brain, the enzyme mediates human cytomegalovirus infection, the enzym regulates the cycle-dependent bioavailability of interleukin-8 in the endometrium, the enzyme's activity is regulated by estrogen | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Lactococcus lactis | - |
gene pepN | - |
Lymantria dispar | - |
gypsy moth | - |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
glycoprotein | - |
Oryctolagus cuniculus |
glycoprotein | heavily glycosylated | Sus scrofa |
glycoprotein | the enzyme contains 9 potential N-glycosylation sites | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
native enzyme from kidney and from small intestine, the kidney brush border enzyme is purified by proteinase treatment or detergent solubilization and chromatographical steps, e.g. affinity chromatography on a 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate resin followed by a concanavalin A resin, the small intestinal enzyme is purified by immunoadsorbent chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | - |
Sus scrofa | - |
blood vessel | lung endothelium | Sus scrofa | - |
blood vessel endothelium | lung blood vessels | Sus scrofa | - |
brain | - |
Homo sapiens | - |
brain | endothelial cells, synaptic membranes of astro- and pericytes | Sus scrofa | - |
granulocyte | - |
Homo sapiens | - |
hematopoietic cell | CD13 | Homo sapiens | - |
hematopoietic stem cell | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
kidney | - |
Sus scrofa | - |
kidney | - |
Oryctolagus cuniculus | - |
liver | - |
Sus scrofa | - |
lung | located on endothelial cells in blood vessels | Sus scrofa | - |
lymphocyte | - |
Homo sapiens | - |
melanoma cell | - |
Homo sapiens | - |
monocyte | - |
Homo sapiens | - |
additional information | wide tissue distribution | Homo sapiens | - |
additional information | wide tissue distribution | Rattus norvegicus | - |
additional information | wide tissue distribution | Sus scrofa | - |
additional information | wide tissue distribution | Oryctolagus cuniculus | - |
mucosa | - |
Sus scrofa | - |
myeloid leukemia cell | - |
Homo sapiens | - |
pericyte | - |
Sus scrofa | - |
placenta | - |
Sus scrofa | - |
prostate cancer cell | - |
Homo sapiens | - |
small intestine | mucosa | Sus scrofa | - |
T-lymphocyte | activated | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cys-Gly + H2O | - |
Homo sapiens | Cys + Gly | - |
? | |
Cys-Gly + H2O | - |
Rattus norvegicus | Cys + Gly | - |
? | |
Cys-Gly + H2O | - |
Sus scrofa | Cys + Gly | - |
? | |
Cys-Gly + H2O | - |
Oryctolagus cuniculus | Cys + Gly | - |
? | |
enkephalin + H2O | hydrolysis of the Tyr1-Gly2 bond | Homo sapiens | ? | - |
? | |
enkephalin + H2O | hydrolysis of the Tyr1-Gly2 bond | Rattus norvegicus | ? | - |
? | |
enkephalin + H2O | hydrolysis of the Tyr1-Gly2 bond | Sus scrofa | ? | - |
? | |
enkephalin + H2O | hydrolysis of the Tyr1-Gly2 bond | Oryctolagus cuniculus | ? | - |
? | |
L-Ala-2-naphthylamide + H2O | - |
Homo sapiens | L-Ala + 2-naphthylamine | - |
? | |
L-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | L-Ala + 2-naphthylamine | - |
? | |
L-Ala-2-naphthylamide + H2O | - |
Sus scrofa | L-Ala + 2-naphthylamine | - |
? | |
L-Ala-2-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Ala + 2-naphthylamine | - |
? | |
L-Ala-4-nitroanilide + H2O | - |
Homo sapiens | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | - |
Rattus norvegicus | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | - |
Sus scrofa | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | - |
Oryctolagus cuniculus | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Sus scrofa | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Oryctolagus cuniculus | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
additional information | the brain enzyme is involved in termination of the action of neuropeptides, especially of enkephalins and angiotensin III, the kidney enzyme contributes to the extracellular catabolism of glutathione, the intestinal enzyme acts as a receptor for the transmissible gastroenteritis virus, a coronavirus, causing fatal diarrhea in newborn pigs | Sus scrofa | ? | - |
? | |
additional information | the enzyme acts as major receptor for the CryIAc toxin of Bacillus thuringiensis | Lymantria dispar | ? | - |
? | |
additional information | the enzyme in granulocytes may cooperate with neprilysin, the enzyme is involved in the metabolism of certain vasoactive peptides in vascular cells in the brain, the enzyme mediates human cytomegalovirus infection, the enzym regulates the cycle-dependent bioavailability of interleukin-8 in the endometrium, the enzyme's activity is regulated by estrogen | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide | Rattus norvegicus | ? | - |
? | |
additional information | substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide | Oryctolagus cuniculus | ? | - |
? | |
additional information | substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide. Glu350 is involved in catalysis, the enzyme in lung is identical with the p146 type II alveolar epithelial cell antigen | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Oryctolagus cuniculus |
More | the enzyme contains a small cytoplasmic domain, a 24-amino-acid hydrophobic segment close to the N-terminus which serves as membrane anchor, and the bulk domain including the active site as ectodomain | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase M | - |
Sus scrofa |
aminopeptidase N | - |
Homo sapiens |
aminopeptidase N | - |
Rattus norvegicus |
aminopeptidase N | - |
Sus scrofa |
aminopeptidase N | - |
Oryctolagus cuniculus |
aminopeptidase N | - |
Lactococcus lactis |
aminopeptidase N | - |
Lymantria dispar |
CD13 | cluster differentiation antigen | Homo sapiens |
Cys-Gly-dipeptidase | - |
Sus scrofa |
cysteinyl-glycinase | - |
Sus scrofa |
mAAP | - |
Homo sapiens |
mAAP | - |
Rattus norvegicus |
mAAP | - |
Sus scrofa |
mAAP | - |
Oryctolagus cuniculus |
mAAP | - |
Lactococcus lactis |
mAAP | - |
Lymantria dispar |
p146 type II alveolar epithelial cell antigen | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | dependent on substrate concentration | Homo sapiens |
7 | 7.5 | dependent on substrate concentration | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
amastatin | preincubation of the enzyme with the inhibitor | Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sus scrofa | about | - |
5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00005 | - |
IC50: 50 nM | Sus scrofa | probestin | |
0.002 | - |
IC50: 0.002 mM, specific inhibitor | Sus scrofa | actinonin |