Cloned (Comment) | Organism |
---|---|
gene pepN, DNA and amino acid sequence determination and analysis, overvexpression | Streptomyces lividans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Streptomyces lividans | |
EDTA | - |
Streptomyces lividans | |
additional information | no inhibition by PMSF | Streptomyces lividans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | the enzyme contains no secretion signal sequence | Streptomyces lividans | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metallopeptidase, zinc-binding motif HELAH | Streptomyces lividans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
gel filtration | Streptomyces lividans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces lividans | the enzyme might be involved in the turnover of intracellular peptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
gene pepN | - |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate fractionation, ion exchange chromatography and gel filtration | Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-4-nitroanilide + H2O | low activity | Streptomyces lividans | Gly + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | - |
Streptomyces lividans | L-Ala + 4-nitroaniline | - |
? | |
L-Arg-2-naphthylamide + H2O | - |
Streptomyces lividans | L-Arg + 2-naphthylamine | - |
? | |
L-Arg-4-nitroanilide + H2O | - |
Streptomyces lividans | L-Arg + 4-nitroaniline | - |
? | |
L-Leu-2-naphthylamide + H2O | - |
Streptomyces lividans | L-Leu + 2-naphthylamine | - |
? | |
L-Leu-4-nitroanilide + H2O | best substrate | Streptomyces lividans | L-Leu + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | - |
Streptomyces lividans | L-Lys + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | - |
Streptomyces lividans | L-Met + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | slow activity | Streptomyces lividans | L-Phe + 4-nitroaniline | - |
? | |
L-Pro-4-nitroanilide + H2O | low activity | Streptomyces lividans | L-Pro + 4-nitroaniline | - |
? | |
L-Ser-4-nitroanilide + H2O | slow activity | Streptomyces lividans | L-Ser + 4-nitroaniline | - |
? | |
additional information | the enzyme might be involved in the turnover of intracellular peptides | Streptomyces lividans | ? | - |
? | |
additional information | no activity with L-Val-4-nitroanilide, L-Glu-4-nitroanilide, and L-Asp-4-nitroanilide, no activity with N-alpha-benzoyl-L-Arg-2-naphthylamide | Streptomyces lividans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 95000, SDS-PAGE | Streptomyces lividans |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase N | - |
Streptomyces lividans |
More | the enzyme belongs to the peptidase family M1 | Streptomyces lividans |
PepN | - |
Streptomyces lividans |