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Literature summary for 3.4.11.2 extracted from

  • Butler, M.J.
    Aminopeptidase N (Streptomyces lividans) (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 322-323.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene pepN, DNA and amino acid sequence determination and analysis, overvexpression Streptomyces lividans

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Streptomyces lividans
EDTA
-
Streptomyces lividans
additional information no inhibition by PMSF Streptomyces lividans

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular the enzyme contains no secretion signal sequence Streptomyces lividans 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metallopeptidase, zinc-binding motif HELAH Streptomyces lividans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
gel filtration Streptomyces lividans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces lividans the enzyme might be involved in the turnover of intracellular peptides ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
gene pepN
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate fractionation, ion exchange chromatography and gel filtration Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-4-nitroanilide + H2O low activity Streptomyces lividans Gly + 4-nitroaniline
-
?
L-Ala-4-nitroanilide + H2O
-
Streptomyces lividans L-Ala + 4-nitroaniline
-
?
L-Arg-2-naphthylamide + H2O
-
Streptomyces lividans L-Arg + 2-naphthylamine
-
?
L-Arg-4-nitroanilide + H2O
-
Streptomyces lividans L-Arg + 4-nitroaniline
-
?
L-Leu-2-naphthylamide + H2O
-
Streptomyces lividans L-Leu + 2-naphthylamine
-
?
L-Leu-4-nitroanilide + H2O best substrate Streptomyces lividans L-Leu + 4-nitroaniline
-
?
L-Lys-4-nitroanilide + H2O
-
Streptomyces lividans L-Lys + 4-nitroaniline
-
?
L-Met-4-nitroanilide + H2O
-
Streptomyces lividans L-Met + 4-nitroaniline
-
?
L-Phe-4-nitroanilide + H2O slow activity Streptomyces lividans L-Phe + 4-nitroaniline
-
?
L-Pro-4-nitroanilide + H2O low activity Streptomyces lividans L-Pro + 4-nitroaniline
-
?
L-Ser-4-nitroanilide + H2O slow activity Streptomyces lividans L-Ser + 4-nitroaniline
-
?
additional information the enzyme might be involved in the turnover of intracellular peptides Streptomyces lividans ?
-
?
additional information no activity with L-Val-4-nitroanilide, L-Glu-4-nitroanilide, and L-Asp-4-nitroanilide, no activity with N-alpha-benzoyl-L-Arg-2-naphthylamide Streptomyces lividans ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 95000, SDS-PAGE Streptomyces lividans

Synonyms

Synonyms Comment Organism
aminopeptidase N
-
Streptomyces lividans
More the enzyme belongs to the peptidase family M1 Streptomyces lividans
PepN
-
Streptomyces lividans