Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.11.2: membrane alanyl aminopeptidase

This is an abbreviated version!
For detailed information about membrane alanyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.2

Reaction

Release of an N-terminal amino acid, Xaa-/-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide =

Synonyms

AAP, Aap 1' aminopeptidase, Aap1 aminopeptidase, Aap1', AlaAP, alanine aminopeptidase, alanine-specific aminopeptidase, alanine/arginine aminopeptidase, alanyl aminopeptidase, alanyl-aminopeptidase, Alpha-aminoacylpeptide hydrolase, amino-oligopeptidase, amino-peptidase N, aminopeptidase D, aminopeptidase M, aminopeptidase M II, aminopeptidase N, aminopeptidase N type 1, aminopeptidase N/CD13, aminopeptidase N3, aminopeptidase, microsomal, aminopeptidase-N, ANPEP, AP-N, Ap1, Ap2, APAse D, APase2, Ape, APN, APN-1, APN-PI, APN/CD13, APN1, APN2, APN3, APN96, basic aminopeptidase, beta-alanyl aminopeptidase, CD13, Cl--activated aminopeptidase, CryIA(C) receptor, Cys-Gly-dipeptidase, cysteinyl-glycinase, dipeptidase, DP, EC 3.4.1. 2, EC 3.4.1.2, EC 3.4.3.5, ENPEP, ePepN, extracellular aminopeptidase, GP 130, GP150, L,(L/D)-aminopeptidase, L-alanine aminopeptidase, LAP, lap3, LeuAP M, leucine aminopeptidase, Leukemia antigen CD13, Lys-AP, Lysyl aminopeptidase, M1 alanine aminopeptidase, mAAP, membrane alanyl aminopeptidase N, membrane alanyl-aminopeptidase, Membrane glycoprotein H11, Membrane protein p161, Microsomal aminopeptidase, microsomal leucine aminopeptidase, More, Myeloid plasma membrane glycoprotein CD13, neutral aminopeptidase, NmAPN, p146, p146 type II alveolar epithelial cell antigen, p161, particle-bound aminopeptidase, PepN, peptidase N, plant aminopeptidase N, puromycin-insensitive neutral aminopeptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.2 membrane alanyl aminopeptidase

Specific Activity

Specific Activity on EC 3.4.11.2 - membrane alanyl aminopeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00051
-
plasma enzyme, 20°C
0.0016
-
plasma enzyme, 20°C
0.0017
-
plasma enzyme, 37°C
0.0033
-
plasma enzyme, 37°C
0.0035
-
normal healthy rats, membrane fraction of heart, APN-PI
0.0043
-
plasma enzyme, 20°C
0.0081
-
plasma enzyme, 37°C
0.0086
-
normal healthy rats, membrane fraction of adrenal gland, APN-PI
0.0115
-
normal healthy rats, membrane fraction of kidney medulla, APN-PI
0.019
-
immobilized enzyme, substrate L-Phe-4-nitroanilide
0.021
-
immobilized enzyme, substrate L-Tyr-4-nitroanilide
0.023
-
immobilized enzyme, substrates L-Ala-4-nitroanilide or L-Arg-4-nitroanilide
0.0238
-
normal healthy rats, membrane fraction of lung, APN-PI
0.044
using L-Phe-L-Phe as substrate, in 25 mM sodium phosphate at pH 7.5
0.046
using L-Tyr-L-Phe as substrate, in 25 mM sodium phosphate at pH 7.5
0.0891
-
normal healthy rats, membrane fraction of kidney cortex, APN-PI
0.12
-
free enzyme, substrate L-Phe-4-nitroanilide
0.142
using L-Lys-L-Phe as substrate, in 25 mM sodium phosphate at pH 7.5
0.15
-
free enzyme, substrate L-Tyr-4-nitroanilide
0.154
using L-Ala-L-Phe as substrate, in 25 mM sodium phosphate at pH 7.5
0.18
-
free enzyme, substrate L-Arg-4-nitroanilide
0.19
-
free enzyme, substrate L-Ala-4-nitroanilide
0.214
using L-Arg-L-Phe as substrate, in 25 mM sodium phosphate at pH 7.5
0.36
-
free enzyme in presence of Tween 80, substrate L-Phe-4-nitroanilide
0.46
-
free enzyme in presence of Tween 80, substrate L-Tyr-4-nitroanilide
0.55
-
free enzyme in presence of Tween 80, substrate L-Arg-4-nitroanilide
0.57
-
free enzyme in presence of Tween 80, substrate L-Ala-4-nitroanilide
0.6
-
L-cystinyl-bis-Gly as substrate
0.78
-
Gly-2-naphthylamide as substrate
0.83
-
value about, substrate L-Asp-7-amido-4-methylcoumarin
1.03
-
cell extract
1.38
-
cell extract
1.6
-
Met-2-naphthylamide as substrate
1.6 - 15
-
aminoacyl-4-nitroanilides as substrates
1.67
-
cell extract
1.68
-
Ala-p-nitroanilide as substrate
105
-
purified enzyme
11.5
-
Leu-p-nitroanilide as substrate
11.7
-
value about, substrate L-Gln-7-amido-4-methylcoumarin
116.7
-
value about, substrate L-Leu-7-amido-4-methylcoumarin
13.1
-
cell extract
144.5
-
purified isozyme APN96, substrate L-Leu-4-nitroanilide
15
-
value about, substrate L-Tyr-7-amido-4-methylcoumarin
16.7
-
value about, substrate L-Thr-7-amido-4-methylcoumarin
18.3
-
pH 7.0, 37°C
190
-
Leu-p-nitroanilide as substrate
233.3
-
value about, substrate L-Lys-4-nitroanilide
29
-
Ala-p-nitroanilide as substrate
3 - 3.9
-
Leu5-enkephalin as substrate
3.3
-
value about, substrate L-Trp-7-amido-4-methylcoumarin
3.54
-
Ala-2-naphthylamide as substrate
33.3
-
value about, substrate L-Pro-7-amido-4-methylcoumarin
33.5
-
Ala-Gly as substrate
4
-
Lys-4-methylcoumarin 7-amide as substrate
4.8
-
Leu-2-naphthylamide as substrate
5.7
-
Leu-4-methylcoumarin 7-amide as substrate
53
-
purified enzyme
58.3
-
value about, substrate L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
6.86
-
purified enzyme, substrate L-Ala-2-naphthylamide
6.9
-
Cys-Gly as substrate
659
-
purified enzyme
8.15
-
Phe-Gly as substrate
83.3
-
value about, substrate L-Met-7-amido-4-methylcoumarin
9.1
-
L-alpha-aminobutyryl-Gly as substrate
9.65
-
cell extract
91.7
-
value about, substrate Gly-7-amido-4-methylcoumarin
964
-
purified recombinant enzyme, substrate L-Ala-4-nitroanilide
additional information