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Literature summary for 3.4.11.2 extracted from
- Purification and characterization of a Cl--activated aminopeptidase from bovine skeletal muscle (2006), Biosci. Biotechnol. Biochem., 70, 1110-1117.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 307% activity in the presence of 10 mM | Bos taurus |  |
| DTT | activates 3fold at 10 mM | Bos taurus | |
| EDTA | 146% activity in the presence of 10 mM | Bos taurus | |
| ethanol | 116% activity in the presence of 1 mM | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 21.6% inhibition at 10 mM; 78.4% residual activity in the presence of 10 mM | Bos taurus | |
| bestatin | 3.2% residual activity in the presence of 1 mM; 97% inhibition at 1 mM | Bos taurus | |
| Co2+ | 2% residual activity in the presence of 1 mM; 98% inhibition at 1 mM | Bos taurus | |
| Cu2+ | 1.6% residual activity in the presence of 1 mM; 98.4% inhibition at 1 mM | Bos taurus | |
| Fe3+ | 39.9% residual activity in the presence of 1 mM; 60.1% inhibition at 1 mM | Bos taurus | |
| iodoacetate | 38.8% residual activity in the presence of 10 mM | Bos taurus | |
| iodoacetic acid | 61.2% inhibition at 10 mM | Bos taurus | |
| K+ | 17.7% inhibition at 1 mM; 82.3% residual activity in the presence of 1 mM | Bos taurus | |
| Mg2+ | 45.1% inhibition at 1 mM; 54.9% residual activity in the presence of 1 mM | Bos taurus | |
| Mn2+ | 5% residual activity in the presence of 1 mM; 94.9% inhibition at 1 mM | Bos taurus | |
| pepstatin A | 22.3% inhibition at 1 mM; 77.7% residual activity in the presence of 1 mM | Bos taurus | |
| Phenylmethylsulfonylfluoride | complete inhibition in the presence of 1 mM | Bos taurus | |
| PMSF | complete inhibition at 1 mM | Bos taurus | |
| puromycin | 25.8% residual activity in the presence of 1 mM; 74.2% inhibition at 1 mM | Bos taurus | |
| SCN- | 4.2% residual activity in the presence of 1 mM | Bos taurus | |
| SO42- | 14% residual activity in the presence of 1 mM | Bos taurus | |
| sulfate | 86% inhibition at 1 M | Bos taurus | |
| Thiocyanide | 95.8% inhibition at 1 M | Bos taurus | |
| Zn2+ | 2.2% residual activity in the presence of 1 mM; 97.8% inhibition at 1 mM | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 44% activation at 1 mM | Bos taurus | |
| Ca2+ | 144% activity in the presence of 1 mM | Bos taurus | |
| Cl- | 3.6fold activation at 1 M | Bos taurus | |
| Cl- | 369% activity in the presence of 1 mM | Bos taurus | |
| F- | 107% activity in the presence of 1 mM | Bos taurus | |
| additional information | no effect by F- | Bos taurus | |
| nitrate | 3.25fold activation at 1 M | Bos taurus | |
| NO3- | 325% activity in the presence of 1 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, DEAE-cellulose column chromatography, Sephacryl S-200 gel filtration, phenyl-Sepharose column chromatography, and His-Trap affinity column chromatography | Bos taurus |
| native enzyme 659fold from skeletal muscle by ammonium sulfate fractionation, anion exchange chromatography, hydroxyapatite chromatography, hydrophobic interaction chromatography, and zinc affinity chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Bos taurus | - |
| skeletal muscle | Musculus longissimus thoracis | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 659 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-beta-naphthylamide + H2O | 100% activity, preferred substrate | Bos taurus | Ala + beta-naphthylamine | - |
? | |
| Arg-beta-naphthylamide + H2O | 16.9% activity compared to Ala-beta-naphthylamide | Bos taurus | Arg + beta-naphthylamine | - |
? | |
| L-Ala-2-naphthylamide + H2O | preferred substrate | Bos taurus | L-Ala + 2-naphthylamine | - |
? | |
| L-Arg-2-naphthylamide + H2O | 16.9% of the activity with L-Ala-2-naphthylamide | Bos taurus | L-Arg + 2-naphthylamine | - |
? | |
| L-Leu-2-naphthylamide + H2O | 16.0% of the activity with L-Ala-2-naphthylamide | Bos taurus | L-Leu + 2-naphthylamine | - |
? | |
| L-Lys-2-naphthylamide + H2O | 6.7% of the activity with L-Ala-2-naphthylamide | Bos taurus | L-Lys + 2-naphthylamine | - |
? | |
| L-Met-2-naphthylamide + H2O | 8.9% of the activity with L-Ala-2-naphthylamide | Bos taurus | L-Met + 2-naphthylamine | - |
? | |
| L-Pro-2-naphthylamide + H2O | 16.1% of the activity with L-Ala-2-naphthylamide | Bos taurus | L-Pro + 2-naphthylamine | - |
? | |
| Leu-beta-naphthylamide + H2O | 16% activity compared to Ala-beta-naphthylamide | Bos taurus | Leu + beta-naphthylamine | - |
? | |
| Lys-beta-naphthylamide + H2O | 6.7% activity compared to Ala-beta-naphthylamide | Bos taurus | Lys + beta-naphthylamine | - |
? | |
| Met-beta-naphthylamide + H2O | 8.9% activity compared to Ala-beta-naphthylamide | Bos taurus | Met + beta-naphthylamine | - |
? | |
| additional information | L-Ser-2-naphthylamide is a poor substrate, L-Glu-2-naphthylamide is no substrate | Bos taurus | ? | - |
? | |
| additional information | no activity with Glu-beta-naphthylamide | Bos taurus | ? | - |
? | |
| Pro-beta-naphthylamide + H2O | 16.1% activity compared to Ala-beta-naphthylamide | Bos taurus | Pro + beta-naphthylamine | - |
? | |
| Ser-beta-naphthylamide + H2O | 1.4% activity compared to Ala-beta-naphthylamide | Bos taurus | Ser + beta-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase D | - |
Bos taurus |
| APAse D | - |
Bos taurus |
| Cl--activated aminopeptidase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, purified enzyme, stable | Bos taurus |
| 55 | 65 | stable up to 55°C, but stability is almost lost at 65°C | Bos taurus |
| 65 | - |
10 min, purified enzyme, almost loss of activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bos taurus |
| 7.5 | - |
around pH 7.5 | Bos taurus |
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Release 2023.1 (January 2023)
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