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Literature summary for 3.4.11.2 extracted from

  • Umetsu, H.; Arai, M.; Ota, T.; Kudo, R.; Sugiura, H.; Ishiyama, H.; Sasaki, K.
    Purification and properties of an aminopeptidase from the mid-gut gland of scallop (Patinopecten yessoensis) (2003), Comp. Biochem. Physiol. B, 136B, 935-942.
No PubMed abstract available
Search for more literature for 3.4.11.2

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline the inhibition is completely reversible by Zn2+, but not by other metal ions such as Mg2+ or Ca2+, partially by Co2+ or Mn2+ Mizuhopecten yessoensis
actinonin slight inhibition Mizuhopecten yessoensis
arphamenine A slight inhibition Mizuhopecten yessoensis
bestatin
-
 Mizuhopecten yessoensis
EDTA
-
 Mizuhopecten yessoensis
additional information no inhibition by antipain, E64, leupeptin, chymostatin, PCMB, PMSF, amastatin, and puromycin Mizuhopecten yessoensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 L-Ala-7-amido-4-methylcoumarin pH 7.0, 30°C Mizuhopecten yessoensis
0.032
-
 L-Ala-4-nitroanilide pH 7.0, 30°C Mizuhopecten yessoensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 gel filtration Mizuhopecten yessoensis
61000
-
 1 * 61000, SDS-PAGE Mizuhopecten yessoensis

Organism

Organism UniProt Comment Textmining
Mizuhopecten yessoensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 340fold from midgut gland by acetone extraction, ammonium sulfate fractionation, ion exchange chromatography, isoelectric focusing, and hydrophobic interaction chromatography Mizuhopecten yessoensis

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
 Mizuhopecten yessoensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mizuhopecten yessoensis
53
-
 purified enzyme Mizuhopecten yessoensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Phe-Tyr-Glu + H2O sequential release of N-terminal amino acids Mizuhopecten yessoensis Ala + Phe + Tyr + Glu
-
 ?
L-Ala-4-nitroanilide + H2O
-
 Mizuhopecten yessoensis L-Ala + 4-nitroaniline
-
 ?
L-Ala-7-amido-4-methylcoumarin + H2O
-
 Mizuhopecten yessoensis L-Ala + 7-amino-4-methylcoumarin
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Mizuhopecten yessoensis L-Leu + 4-nitroaniline
-
 ?
L-Leu-7-amido-4-methylcoumarin + H2O
-
 Mizuhopecten yessoensis L-Leu + 7-amino-4-methylcoumarin
-
 ?
L-Met-4-nitroanilide + H2O
-
 Mizuhopecten yessoensis L-Met + 4-nitroaniline
-
 ?
L-Met-7-amido-4-methylcoumarin + H2O
-
 Mizuhopecten yessoensis L-Met + 7-amino-4-methylcoumarin
-
 ?
additional information substrate specificity, the enzyme prefers substrates with Ala or Met residues at the N-terminus, overview Mizuhopecten yessoensis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61000, SDS-PAGE Mizuhopecten yessoensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mizuhopecten yessoensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 L-Ala-7-amido-4-methylcoumarin pH 7.0, 30°C Mizuhopecten yessoensis
9.49
-
 L-Ala-7-amido-4-methylcoumarin pH 7.0, 30°C Mizuhopecten yessoensis
29.5
-
 L-Ala-4-nitroanilide pH 7.0, 30°C Mizuhopecten yessoensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mizuhopecten yessoensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable, purified enzyme Mizuhopecten yessoensis

pI Value

Organism Comment pI Value Maximum pI Value
Mizuhopecten yessoensis isoelectric focusing
-
 5.2