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2,4-dinitrophenyl beta-D-glucopyranoside + H2O
2,4-dinitrophenol + beta-D-glucopyranose
-
-
-
-
?
2,4-dinitrophenyl beta-D-glucopyranoside + H2O
2,4-dinitrophenol + D-glucopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactose + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside
2-nitrophenol + D-galactose
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
-
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
-
-
-
?
2-nitrophenyl beta-D-glucoside + H2O
2-nitrophenol + beta-D-glucose
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + D-xylopyranose
-
-
-
-
?
2-nitrophenyl beta-galactoside + H2O
2-nitrophenol + D-galactose
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
-
laminaribiose
-
?
3beta-[beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyloxy]-20-[beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyloxy]dammar-24-en-12beta-ol + H2O
ginsenoside Rd + D-glucose
-
i.e. ginsenoside Rb1
-
-
?
4-methylumbelliferyl beta-D-fucopyranoside + H2O
4-methylumbelliferone + beta-D-fucopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-fucoside + H2O
4-methylumbelliferol + D-fucose
-
-
-
?
4-methylumbelliferyl beta-D-fucoside + H2O
4-methylumbelliferone + beta-D-fucose
-
-
-
?
4-methylumbelliferyl beta-D-galactopyranoside + H2O
4-methylumbelliferone + beta-D-galactopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
4-methylumbelliferyl beta-D-galacturonate + H2O
4-methylumbelliferone + beta-D-galacturonate
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferol + D-glucose
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
4-methylumbelliferyl beta-D-glucuronic acid + H2O
4-methylumbelliferone + beta-D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferol + D-glucose
-
-
-
?
4-methylumbelliferyl beta-D-mannopyranoside + H2O
4-methylumbelliferone + beta-D-mannopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferol + D-mannose
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
4-methylumbelliferyl beta-D-xylopyranoside + H2O
4-methylumbelliferone + beta-D-xylopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-xyloside + H2O
4-methylumbelliferol + D-xylose
-
-
-
?
4-methylumbelliferyl beta-D-xyloside + H2O
4-methylumbelliferone + beta-D-xylose
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O
4-methylumbelliferone + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
4-nitrophenyl beta-D-galactoside
4-nitrophenol + beta-D-galactose
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
4-nitrophenyl beta-D-glucoside
4-nitrophenol + beta-D-glucose
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenyl + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-mannoside + H2O
4-nitrophenol + D-mannose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + D-xylose
-
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
-
-
-
-
?
cellobiose + H2O
2 beta-D-glucose
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
-
?
cellobiose + H2O
beta-D-glucose + beta-D-glucose
-
-
-
-
?
cellobiose + H2O
D-glucose
cellobiose + H2O
D-glucose + D-glucose
cellotetraose + H2O
cellotriose + D-glucose
-
-
-
-
?
gentiobiose + H2O
2 beta-D-glucose
-
-
-
-
?
ginsenoside C-Mc1 + H2O
ginsenoside C-K + D-glucose + L-arabinofuranose
ginsenoside C-O + H2O
ginsenoside C-K + D-glucose + L-arabinopyranose
-
-
-
?
ginsenoside C-Y + H2O
ginsenoside C-K + L-arabinopyranose
-
-
-
?
ginsenoside F2 + H2O
ginsenoside C-K + D-glucose
highest activity
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + D-glucose
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + D-glucose + L-arabinopyranose
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside Rd + L-arabinopyranose
-
i.e. 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D glucopyranosyloxy]-20-[alpha-L-arabinopyranosyl-(1->6)-beta-D glucopyranosyloxy]dammar-24-en-12beta-ol
-
-
?
ginsenoside Rc + H2O
ginsenoside C-K + D-glucose + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
ginsenoside Mc + L-arabinofuranose
-
i.e. 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D glucopyranosyloxy]-20-[alpha-L-arabinofuranosyl-(1->6)-beta-D glucopyranosyloxy]dammar-24-en-12beta-ol
-
-
?
ginsenoside Rd + H2O
ginsenoside C-K + ?
-
-
-
?
ginsenoside Rd + H2O
ginsenoside C-K + D-glucose
-
-
-
?
ginsenoside Rd + H2O
ginsenoside K + D-glucose
-
i.e. 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyloxy]-20-(beta-D-glucopyranosyloxy)dammar-24-en-12beta-ol
-
-
?
lactose + H2O
beta-D-glucose + beta-D-galactose
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
laminaribiose + H2O
3 beta-D-glucose
-
-
-
-
?
laminaribiose + H2O
?
-
-
-
-
?
methyl beta-D-galactoside
methanol + D-galactose
oleuropein + H2O
oleuropein aglycone + D-glucopyranose
-
i.e. (4S,5E,6S)-4-[2-[2-(3,4-dihydroxyphenyl)ethoxy]-2-oxoethyl]-5-ethylidene-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)-2-tetrahydropyranyl]oxy]-4H-pyran-3-carboxylic acid methyl ester. The biotransformation produces unstable aglycone species formed by oleuropein hydrolysis that gives rise to the formation of hydroxytyrosol, at the operative temperatures of the bioreactor. The results of the biotransformation at 70ưC showed that the main products are hydroxytyrosol, and glucose, being the oleuropein aglycone present in low amount at the end of reaction
-
-
?
rebaudioside A + H2O
steviol + D-glucose
rubusoside + H2O
steviol + D-glucose
stevioside + H2O
steviol + D-glucose
-
-
-
?
additional information
?
-
2-nitrophenyl beta-D-galactopyranoside + H2O

2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
719097, 720966, 721007, 721008, 722128, 723982, 724154, 724623, 724903, 725589, 726456 -
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside

2-nitrophenol + D-galactose
-
-
-
r
2-nitrophenyl beta-D-galactoside
2-nitrophenol + D-galactose
-
-
-
r
2-nitrophenyl beta-D-glucoside + H2O

2-nitrophenol + beta-D-glucose
-
-
-
-
?
2-nitrophenyl beta-D-glucoside + H2O
2-nitrophenol + beta-D-glucose
-
-
-
-
?
2-nitrophenyl beta-galactoside + H2O

2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O

4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O

4-methylumbelliferol + D-glucose
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferol + D-glucose
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O

4-methylumbelliferone + beta-D-glucose
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O

4-methylumbelliferone + beta-D-mannose
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O

4-nitrophenol + alpha-L-fucopyranose
-
14% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
14% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O

4-nitrophenol + beta-D-fucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
73% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
73% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucoside + H2O

4-nitrophenol + beta-D-fucose
-
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O

4-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O

4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactoside

4-nitrophenol + beta-D-galactose
LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside
-
-
?
4-nitrophenyl beta-D-galactoside
4-nitrophenol + beta-D-galactose
LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside
-
-
?
4-nitrophenyl beta-D-galactoside + H2O

4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
the enzyme shows constant donor selectivity (Vmax ratio of the 4-nitrophenyl-beta-D-glucoside and 4-nitrophenyl-beta-D-galactoside) as the temperature increased
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O

4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O

4-nitrophenol + D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
52% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
52% of the activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O

4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucoside

4-nitrophenol + beta-D-glucose
LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside
-
-
?
4-nitrophenyl beta-D-glucoside
4-nitrophenol + beta-D-glucose
LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside
-
-
?
4-nitrophenyl beta-D-glucoside + H2O

4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
the enzyme shows constant donor selectivity (Vmax ratio of the 4-nitrophenyl-beta-D-glucoside and 4-nitrophenyl-beta-D-galactoside) as the temperature increases
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O

4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
highest activity
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
highest activity
-
-
?
cellobiose + H2O

D-glucose
-
-
-
-
?
cellobiose + H2O
D-glucose
-
-
-
-
?
cellobiose + H2O

D-glucose + D-glucose
-
-
-
-
?
cellobiose + H2O
D-glucose + D-glucose
-
-
-
?
cellobiose + H2O
D-glucose + D-glucose
-
-
-
?
cellotetraose + H2O

?
-
-
-
-
?
cellotetraose + H2O
?
-
-
-
-
?
cellotriose + H2O

?
-
-
-
-
?
cellotriose + H2O
?
-
-
-
-
?
ginsenoside C-Mc + H2O

?
-
-
-
?
ginsenoside C-Mc + H2O
?
-
-
-
?
ginsenoside C-Mc1 + H2O

ginsenoside C-K + D-glucose + L-arabinofuranose
-
-
-
?
ginsenoside C-Mc1 + H2O
ginsenoside C-K + D-glucose + L-arabinofuranose
-
-
-
?
hesperidin + H2O

?
-
-
-
?
hesperidin + H2O
?
-
-
-
?
lactose + H2O

D-glucose + D-galactose
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
-
-
-
?
lactose + H2O
D-glucose + D-galactose
-
-
-
r
lactose + H2O
D-glucose + D-galactose
-
-
-
r
lactose + H2O
D-glucose + D-galactose
-
-
-
?
lactose + H2O
D-glucose + D-galactose
-
-
-
?
methyl beta-D-galactoside

methanol + D-galactose
-
-
-
r
methyl beta-D-galactoside
methanol + D-galactose
-
-
-
r
naringin + H2O

?
-
-
-
?
rebaudioside A + H2O

steviol + D-glucose
negligible activity
-
-
?
rebaudioside A + H2O
steviol + D-glucose
negligible activity
-
-
?
rubusoside + H2O

steviol + D-glucose
44.2% yield
-
-
?
rubusoside + H2O
steviol + D-glucose
44.2% yield
-
-
?
additional information

?
-
-
hydrolase with broad specificity and noticeable glucan 1 -> 4-beta-D-glucosidase activity
-
-
?
additional information
?
-
-
kinetic studies of the reactions of galactosylated enzyme intermediates with a range of nucleophiles
-
-
?
additional information
?
-
-
broad specificity and remarkable glucan-1,4-beta-D-glucosidase (exocellobiase) activity
-
-
?
additional information
?
-
-
the enzyme hydrolyzes a variety of low-molecular-weight, beta-linked glycosides. Chromogenic beta-D-galactosides and beta-D-glucosides are hydrolyzed at a common active site. beta-Glucosides and beta-fucosides represent the preferred substrates
-
-
?
additional information
?
-
-
wide substrate specificity. Active on aryl-beta-galactose, -fucose, -glucose and -xylose and on di- and oligosaccharides. Among glucose dimers are hydrolysed in the order of decreasing activity: beta-(1,3), beta-(1,4), beta-(1,6). Ssbeta-Gly is an exo-acting enzyme with a preference for cellotetraose
-
-
?
additional information
?
-
-
catalyzes transglycosylation reaction with with lactose as donor and N-acetyllactosamine as acceptor to synthesize beta-galactooligosaccharides at different temperatures. The main products are beta-D-Gal-1,6-D-GlcNAc, beta-D-Gal-1,4-D-GlcNAc and several oligosaccharides
-
-
?
additional information
?
-
-
collection of information on the global and local dynamics in view of a full understanding of topology and motion hierarchy in proteins by dynamic fluorescence and site-specific labeling EPR
-
-
?
additional information
?
-
-
during lactose conversion at 70ưC galactosyl transfer to acceptors other than water competes efficiently with complete hydrolysis of substrate. This process leads to transient formation of a range of new products, mainly disaccharides and trisaccharides, and shows a marked dependence on initial substrate concentration and lactose conversion. The molar ratio of trisaccharides to disaccharides is maximal at an early stage of reaction and decreases directly proportional to increasing substrate conversion. The major transgalactosylation products of SsbetaGl are beta-D-Galp-(1->3)-Glc and beta-D-Galp-(1->6)-Glc, and beta-D-Galp-(1->3)-lactose and beta-D-Galp-(1-->6)-lactose. The enzyme accumulates beta(1-->6)-linked glycosides, particularly allolactose, at a late stage of reaction
-
-
?
additional information
?
-
-
mutant enzyme E387G catalyzes transglycosylation reactions. In the transglycosylation reactions, 2-nitrophenyl beta-D-glucopyranoside is used as the donor and different aryl and alkyl mono- and disaccharide substrates containing alpha- and beta-glycosidic linkages are used as acceptors. The formation of the beta-1,3 linkage is observed with acceptors containing the 2-nitrophenyl group, while beta-1,6-disaccharides are predominant with 4-nitrophenyl beta-D-glucoside. When a beta linkage of the acceptor is between two sugars, as for the formation of trisaccharides, the beta-1,3 acceptors possess affinity for the donor active site and are also partially hydrolyzed. The beta-1,4-based acceptor furnish only linear trisaccharides, albeit in very poor yield. The presence of alpha linkages in the acceptors induces beta-1,6 glycosylation despite the nature ofthe aglycons. In these cases, the yield is higher with the increasing lipophilic nature of the aglycon; however, the yield depends on the dimensions and nature of this group. In fact, while maltose as free disaccharide givse very poor yields of trisaccharide products, 33% yield is reached with 4-nitrophenyl alpha-D-glucopyranoside
-
-
?
additional information
?
-
the enzyme catalyzes the conversion of lactose to hexyl-beta-D-galactoside in hexanol (transgalactosylation reaction)
-
-
?
additional information
?
-
-
the enzyme catalyzes the enzymatic synthesis of 2-beta-D-galactopyranosyloxyethyl methacrylate starting from 2-hydroxyethyl methacrylate and 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
additional information
?
-
the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates
-
-
?
additional information
?
-
-
the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates
-
-
?
additional information
?
-
-
the enzyme performs the transfer glucose, galactose and fucose from different donors
-
-
?
additional information
?
-
-
the transfer of the galactosyl group from lactose to acceptors such as lactose or D-glucose rather than water is significant for both enzymes and depends on the initial lactose concentration as well as the time-dependent substrate/product ratio during batchwise lactose conversion
-
-
?
additional information
?
-
-
the enzyme shows broad specificity for beta-D-gluco-, fuco- and galactosides. It hydrolyses a large number of beta-linked glycoside dimers and oligomers; chromogenic beta-glucosides and beta-fucosides are the preferred substrates, and kinetic analysis indicates that they bind to a common catalytic site. The order of catalytic efficiency is beta 1-3 > beta 1-4 > beta 1-6 and cellotetraose > cellotriose > cellobiose for glucose dimers and oliogomers respectively. The cleavage occurres at the non-reducing end of the oligosaccharide, and the enzyme shows noticeable specificity also for the aglycone part of substrates. The enzyme from Sulfolobus solfataricus strain MT-4 is defined as a true glycosyl hydrolase with remarkable exo-glucosidase activity
-
-
?
additional information
?
-
enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose
-
-
?
additional information
?
-
-
enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose
-
-
?
additional information
?
-
no activity with alpha-L-arabinofuranoside linked to ginsenoside Rc
-
-
?
additional information
?
-
the specific hydrolytic activity for the wild type enzyme follows the order ginsenoside F2 > Rb1 > C-O > C-Y > Rb2 > Rd > C-Mc> C-Mc1
-
-
?
additional information
?
-
no activity with alpha-L-arabinofuranoside linked to ginsenoside Rc
-
-
?
additional information
?
-
the specific hydrolytic activity for the wild type enzyme follows the order ginsenoside F2 > Rb1 > C-O > C-Y > Rb2 > Rd > C-Mc> C-Mc1
-
-
?
additional information
?
-
enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose
-
-
?
additional information
?
-
-
the enzyme performs the transfer glucose, galactose and fucose from different donors
-
-
?
additional information
?
-
-
the enzyme shows broad specificity for beta-D-gluco-, fuco- and galactosides. It hydrolyses a large number of beta-linked glycoside dimers and oligomers; chromogenic beta-glucosides and beta-fucosides are the preferred substrates, and kinetic analysis indicates that they bind to a common catalytic site. The order of catalytic efficiency is beta 1-3 > beta 1-4 > beta 1-6 and cellotetraose > cellotriose > cellobiose for glucose dimers and oliogomers respectively. The cleavage occurres at the non-reducing end of the oligosaccharide, and the enzyme shows noticeable specificity also for the aglycone part of substrates. The enzyme from Sulfolobus solfataricus strain MT-4 is defined as a true glycosyl hydrolase with remarkable exo-glucosidase activity
-
-
?
additional information
?
-
-
the enzyme hydrolyzes a variety of low-molecular-weight, beta-linked glycosides. Chromogenic beta-D-galactosides and beta-D-glucosides are hydrolyzed at a common active site. beta-Glucosides and beta-fucosides represent the preferred substrates
-
-
?
additional information
?
-
the enzyme catalyzes the conversion of lactose to hexyl-beta-D-galactoside in hexanol (transgalactosylation reaction)
-
-
?
additional information
?
-
the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates
-
-
?
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(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
inhibits activity with 4-nitrophenyl beta-D-galactopyranoside
1,4-D-galactonolactone
-
0.1 M, 98% inhibition of beta-galactoside hydrolysis, 90% inhibition of beta-glucoside hydrolysis
1,4-dioxane
-
10% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-fucopyranoside
1,5-D-gluconolactone
-
0.1 M, complete inhibition of beta-galactoside hydrolysis and beta-glucoside hydrolysis
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucoside
-
a mechanism-based inhibitor. 102fold molar excess, 80% inhibition of wild-type enzyme after 30 min and complete after overnight incubation. The E387G mutant enzyme is insensitive to the inhibitor
2,4-dinitrophenyl-beta-2-deoxy-2-fluoro-D-glucopyranoside
-
-
2-phenethyl glucoimidazole
-
-
4-nitrophenyl-beta-D-galactopyranoside
-
at low and intermediate temperatures (from 25ưC to 50ưC), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80ưC) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate
Ba2+
-
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+
butan-2-one
-
10-20 (v/v)
Ca2+
-
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+
conduritol B epoxide
-
i.e. DL-1,2 anhydro-myo-inositol. The inhibitor is covalently bound to E387. Inhibitor-enzyme intermediate complex is formed more rapidly and hydrolyzed at a lower rate than it is for other glycosidases. One molecule of the inhibitor is covalently bound to each enzyme subunit
cyclophellitol
-
highly specific irreversible inhibitor of beta-glycosidases. Structural and dynamic aspects of beta-glycosidase from mesophilic and thermophilic bacteria by multitryptophanyl emission decay studies
D-arabinose
-
0.1 M, 64% inhibition of beta-galactoside hydrolysis, 20% inhibition of beta-glucoside hydrolysis
D-cellobiose
-
0.1 M, 79% inhibition of beta-galactoside hydrolysis, 25% inhibition of beta-glucoside hydrolysis
D-fucose
-
0.1 M, 58% inhibition of beta-galactoside hydrolysis, 23% inhibition of beta-glucoside hydrolysis
D-galactohydroximolactam
-
D-gluconic acid lactone
-
strong competitive inhibitor
DL-1,2 anhydro-myo-inositol
-
the enzyme is fully inactivated at 65ưC in presence of the inhibitor, according to pseudo-first-order kinetics. The process takes place through the formation of a stabilized inhibitor-enzyme intermediate. One molecule of the inhibitor is covalently bound to each enzyme subunit. The inhibitor iss covalently bound to E387
K+
-
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+
lactose
-
0.1 M, 41% inhibition of beta-galactoside hydrolysis, 18% inhibition of beta-glucoside hydrolysis
Li+
-
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+
Mg2+
-
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+
Na+
-
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+
Salicin
-
0.1 M, 97% inhibition of beta-galactoside hydrolysis, 92% inhibition of beta-glucoside hydrolysis
SDS
-
50ưC, 30 min, 0.3% SDS, 40% loss of activity
tetrahydrofuran
-
5% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-fucopyranoside
D-galactose

-
0.1 M, 21% inhibition of beta-galactoside hydrolysis, slight activation of beta-glucoside hydrolysis
D-galactose
-
measured with lactose as the substrate
D-glucose

-
0.1 M, 37% inhibition of beta-galactoside hydrolysis, slight activation of beta-glucoside hydrolysis
D-glucose
inhibits activity with 4-nitrophenyl beta-D-galactopyranoside
D-glucose
competitive inhibitor
additional information

-
soluble and immobilized enzyme show no inhibition by glucose
-
additional information
the activities of the wild-type enzyme in 30% acetonitrile, butanone and acetone are higher than those in dimethyl sulfoxide, dimethylformamide and 1,4-dioxane. The low boiling point of acetone limits the application of this solvent in thermophilic enzyme reactions
-
additional information
-
the activities of the wild-type enzyme in 30% acetonitrile, butanone and acetone are higher than those in dimethyl sulfoxide, dimethylformamide and 1,4-dioxane. The low boiling point of acetone limits the application of this solvent in thermophilic enzyme reactions
-
additional information
-
cations can cause a strong attenuation of the ion pair interactions E474Ć¢ĀĀK72 and D473Ć¢ĀĀR402, with consequent partial dissociation of the tetrameric structure
-
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0.17
2,4-dinitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
0.95 - 5.3
2-nitrophenyl beta-D-galactopyranoside
0.87 - 1.1
2-nitrophenyl beta-D-galactoside
0.5 - 16.4
2-nitrophenyl beta-D-glucopyranoside
0.03 - 1.01
2-Nitrophenyl beta-D-glucoside
2.25
2-nitrophenyl beta-galactoside
at pH 6.0 and 80ưC
3.9
2-nitrophenyl-beta-D-galactopyranoside
-
-
13
2-Nitrophenyl-beta-D-glucopyranoside
-
pH 5.5, 65ưC
0.011 - 0.41
4-methylumbelliferyl beta-D-fucopyranoside
0.011 - 18.6
4-methylumbelliferyl beta-D-fucoside
0.066 - 2.2
4-methylumbelliferyl beta-D-galactopyranoside
0.066 - 5.28
4-methylumbelliferyl beta-D-galactoside
1.3 - 3.46
4-methylumbelliferyl beta-D-galacturonate
0.046 - 1.61
4-methylumbelliferyl beta-D-glucopyranoside
0.046 - 22.1
4-methylumbelliferyl beta-D-glucoside
1.3 - 1.4
4-methylumbelliferyl beta-D-glucuronic acid
1.3
4-methylumbelliferyl beta-D-glucuronide
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.036 - 0.9
4-methylumbelliferyl beta-D-mannopyranoside
0.036 - 24.2
4-methylumbelliferyl beta-D-mannoside
0.068 - 1.26
4-methylumbelliferyl beta-D-xylopyranoside
0.13 - 13.7
4-methylumbelliferyl beta-D-xyloside
2.6
4-methylumbelliferyl-beta-D-glucopyranoside
-
pH 5.5, 65ưC
1.2
4-methylumbelliferyl-beta-D-xylopyranoside
-
pH 5.5, 65ưC
0.095 - 0.45
4-nitrophenyl beta-D-fucopyranoside
0.09 - 18.6
4-nitrophenyl beta-D-fucoside
0.77 - 4.79
4-nitrophenyl beta-D-galactopyranoside
0.57 - 47
4-nitrophenyl beta-D-galactoside
0.095 - 0.6
4-nitrophenyl beta-D-glucopyranoside
0.03 - 22.1
4-nitrophenyl beta-D-glucoside
24.2
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
5.1 - 13.7
4-nitrophenyl beta-D-xyloside
0.3
4-nitrophenyl cellobioside
0.7
4-nitrophenyl-beta-D-fucoside
-
-
4.3
4-nitrophenyl-beta-D-galactopyranoside
-
-
0.085 - 54
4-nitrophenyl-beta-D-glucopyranoside
25
4-nitrophenyl-beta-D-xylopyranoside
-
pH 5.5, 65ưC
0.3
4-nitrophenyl-cellobioside
-
-
0.57 - 1.93
ginsenoside Rb1
0.52 - 1.59
ginsenoside Rd
192
methyl beta-D-galactoside
release of methanol, pH 7.5, 80ưC
17.21
stevioside
at pH 6.0 and 80ưC
additional information
additional information
-
0.95
2-nitrophenyl beta-D-galactopyranoside

-
pH 6.5, 65ưC, wild-type enzyme
0.95
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme R488A
0.99
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
1.14
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 60ưC
1.47
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme R489A
1.5
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1.53
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme E206Q
1.9
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme His489
1.97
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme delVal484-His489
1.99
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
3.9
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, recombinant enzyme
3.9
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme
4
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, wild-type enzyme
4.1
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme
5
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, enzyme immobilized on chitosan activated with glutaraldehyde
5.3
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, immobilized enzyme
0.87
2-nitrophenyl beta-D-galactoside

-
pH 5.4, 75ưC
1.1
2-nitrophenyl beta-D-galactoside
release of 2-nitrophenol, pH 7.5, 80ưC
0.5
2-nitrophenyl beta-D-glucopyranoside

-
wild-type, pH 6.5, 65ưC, presence of 2 M formate
1.01
2-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
1.01
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65ưC
1.1
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.0, 65ưC
1.17
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.5, 65ưC, presence of 2 M formate
2.2
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 5.0, 65ưC
4.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 4.0, 65ưC
16.4
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 3.0, 65ưC
0.03
2-Nitrophenyl beta-D-glucoside

-
pH 6.5, 65ưC, mutant enzyme E206Q
1.01
2-Nitrophenyl beta-D-glucoside
-
pH 6.5, 65ưC, wild-type enzyme
0.011
4-methylumbelliferyl beta-D-fucopyranoside

-
pH 6.5, 80ưC, wild-type enzyme
0.023
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.34
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
0.41
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
0.011
4-methylumbelliferyl beta-D-fucoside

pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.011
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, wild-type enzyme
0.21
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45ưC, wild-type enzyme
0.34
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
0.34
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, mutant enzyme E432C
0.41
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
0.41
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, mutant enzyme W433C
18.6
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45ưC, mutant enzyme W433C
0.066
4-methylumbelliferyl beta-D-galactopyranoside

-
pH 6.5, 80ưC, wild-type enzyme
0.083
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.47
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
2.2
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
0.066
4-methylumbelliferyl beta-D-galactoside

pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.066
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, wild-type enzyme
0.47
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
0.47
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, mutant enzyme E432C
0.57
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, wild-type enzyme
0.81
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, mutant enzyme E432C
2.2
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
2.2
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, mutant enzyme W433C
5.28
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, mutant enzyme W433C
1.3
4-methylumbelliferyl beta-D-galacturonate

pH 6.5, 80ưC, wild-type enzyme
2.72
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45ưC, mutant enzyme W433C
3.46
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45ưC, mutant enzyme E432C
0.046
4-methylumbelliferyl beta-D-glucopyranoside

-
pH 6.5, 80ưC, wild-type enzyme
0.068
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.34
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
1.61
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
0.046
4-methylumbelliferyl beta-D-glucoside

pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.046
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, wild-type enzyme
0.15
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, wild-type enzyme
0.34
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
0.34
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, mutant enzyme E432C
0.51
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, mutant enzyme E432C
1.61
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
1.61
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, mutant enzyme W433C
22.1
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, mutant enzyme W433C
1.3
4-methylumbelliferyl beta-D-glucuronic acid

-
pH 6.5, 80ưC, wild-type enzyme
1.4
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80ưC, mutant enzyme M439C
0.036
4-methylumbelliferyl beta-D-mannopyranoside

-
pH 6.5, 80ưC, wild-type enzyme
0.042
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.18
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
0.9
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
0.036
4-methylumbelliferyl beta-D-mannoside

pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.036
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, wild-type enzyme
0.18
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, mutant enzyme W433C
0.9
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
0.9
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, mutant enzyme E432C
24.2
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45ưC, wild-type enzyme
0.068
4-methylumbelliferyl beta-D-xylopyranoside

-
pH 6.5, 80ưC, mutant enzyme M439C
0.13
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
0.59
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
1.26
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
0.13
4-methylumbelliferyl beta-D-xyloside

pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.13
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, wild-type enzyme
0.59
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
0.59
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, mutant enzyme W433C
1.26
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
1.26
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, mutant enzyme E432C
5.1
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45ưC, wild-type enzyme
13.7
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45ưC, mutant enzyme E432C
0.095
4-nitrophenyl beta-D-fucopyranoside

-
pH 6.5, 60ưC
0.13
4-nitrophenyl beta-D-fucopyranoside
-
pH 4.9, 77ưC
0.4
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.45
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
0.09
4-nitrophenyl beta-D-fucoside

-
pH 6.5, 65ưC, mutant enzyme E206Q
0.21
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
0.45
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 65ưC, wild-type enzyme
0.7
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 75ưC, recombinant enzyme
1
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 75ưC, wild-type enzyme
18.6
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
0.77
4-nitrophenyl beta-D-galactopyranoside

-
pH 4.9, 77ưC
0.83
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 60ưC
0.84
4-nitrophenyl beta-D-galactopyranoside
-
pH 7.5, 75ưC
1.17
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
1.57
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 70ưC
2.33
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
2.51
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
4.3
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, recombinant enzyme
4.7
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, wild-type enzyme
4.79
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme E206Q
0.57
4-nitrophenyl beta-D-galactoside

pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
0.59
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 30ưC
0.72
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 60ưC
0.77
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 65ưC
0.81
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.82
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 40ưC
1.09
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 50ưC
1.61
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 75ưC
3.16
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 85ưC
5.28
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
6.2
4-nitrophenyl beta-D-galactoside
-
pH 6.5, 90ưC
21
4-nitrophenyl beta-D-galactoside
-
pH 6.5, 70ưC
47
4-nitrophenyl beta-D-galactoside
-
pH 6.5, 50ưC
0.095
4-nitrophenyl beta-D-glucopyranoside

-
pH 4.9, 77ưC
0.12
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 70ưC
0.16
4-nitrophenyl beta-D-glucopyranoside
pH 6.5, 37ưC
0.2
4-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 60ưC
0.45
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.6
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
0.03
4-nitrophenyl beta-D-glucoside

-
pH 6.5, 65ưC, mutant enzyme E206Q
0.15
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
0.3
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 65ưC, wild-type enzyme
0.5
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 75ưC, recombinant enzyme
0.5
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 75ưC, wild-type enzyme
0.51
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
2.04
4-nitrophenyl beta-D-glucoside
at pH 6.0 and 80ưC
4.2
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 90ưC
13
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 70ưC
21
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 50ưC
22.1
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
5.1
4-nitrophenyl beta-D-xyloside

pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
13.7
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.3
4-nitrophenyl cellobioside

-
pH 6.5, 75ưC, recombinant enzyme
0.3
4-nitrophenyl cellobioside
-
pH 6.5, 75ưC, wild-type enzyme
0.085
4-nitrophenyl-beta-D-glucopyranoside

-
pH 6.0, 60ưC, 1 bar
0.16
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 500 bar
0.41
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 750 bar
0.5
4-nitrophenyl-beta-D-glucopyranoside
-
-
0.55
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 1000 bar
0.86
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 1500 bar
54
4-nitrophenyl-beta-D-glucopyranoside
-
pH 5.5, 65ưC
13.5
cellobiose

pH 5.0, 70ưC
20
cellobiose
-
pH 7.0, 75ưC, enzyme immobilized on chitosan activated with glutaraldehyde
21.54
cellobiose
-
pH and temperature not specified in the publication, recombinant enzyme
21.6
cellobiose
-
pH and temperature not specified in the publication, immobilized enzyme
22.4
cellobiose
-
pH and temperature not specified in the publication, native enzyme
31.5
cellobiose
-
pH 7.0, 75ưC, soluble enzyme
32.7
cellobiose
-
pH and temperature not specified in the publication, soluble enzyme
0.57
ginsenoside Rb1

wild type enzyme, at pH 5.5 and 95ưC
1.93
ginsenoside Rb1
mutant enzyme W361F, at pH 5.5 and 95ưC
0.52
ginsenoside Rd

wild type enzyme, at pH 5.5 and 95ưC
1.59
ginsenoside Rd
mutant enzyme W361F, at pH 5.5 and 95ưC
15.82
lactose

at pH 6.0 and 80ưC
37.9
lactose
pH 5.0, 70ưC
44
lactose
-
pH 5.5, 70ưC, enzyme immobilized on Eupergit C
63
lactose
-
pH 5.5, 70ưC, soluble enzyme
70
lactose
-
pH 5.5, 70ưC, enzyme immobilized on chitosan
92
lactose
-
pH 5.5, 80ưC
131
lactose
-
pH 5.5, 70ưC, enzyme immobilized on controlled pore glass
196
lactose
release of D-glucose, pH 7.5, 80ưC
1.29
laminaribiose

-
pH and temperature not specified in the publication, native enzyme
1.4
laminaribiose
-
pH and temperature not specified in the publication, recombinant enzyme
169
oleuropein

-
pH 7.0, 60ưC, soluble enzyme
240.4
oleuropein
-
pH 7.0, 70ưC, soluble enzyme
additional information
additional information

-
kinetic studies of the reactions of galactosylated enzyme intermediates with a range of nucleophiles
-
additional information
additional information
-
KM increases with temperature: the increase is moderate at lower temperatures (from 30ưC to 65ưC) and more evident between 65ưC and 85ưC. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverĆ¢ĀĀBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated
-
additional information
additional information
-
kinetic constant values for soluble enzyme and for enzyme immobilized on chitosan activated with glutaraldehyde are comparable
-
additional information
additional information
-
kinetic constants for transglycosylation reactions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
275
2,4-dinitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
34 - 2437
2-nitrophenyl beta-D-galactopyranoside
1300 - 2400
2-nitrophenyl beta-D-galactoside
20.7 - 901.4
2-nitrophenyl beta-D-glucopyranoside
4.5 - 252
2-Nitrophenyl beta-D-glucoside
24
2-nitrophenyl beta-galactoside
at pH 6.0 and 80ưC
4.7
2-Nitrophenyl-beta-D-glucopyranoside
-
pH 5.5, 65ưC
18 - 91
4-methylumbelliferyl beta-D-fucopyranoside
5.16 - 89
4-methylumbelliferyl beta-D-fucoside
5.4 - 98
4-methylumbelliferyl beta-D-galactopyranoside
0.047 - 98
4-methylumbelliferyl beta-D-galactoside
0.004 - 0.81
4-methylumbelliferyl beta-D-galacturonate
5.1 - 190
4-methylumbelliferyl beta-D-glucopyranoside
0.018 - 140
4-methylumbelliferyl beta-D-glucoside
0.81 - 1.3
4-methylumbelliferyl beta-D-glucuronic acid
0.81
4-methylumbelliferyl beta-D-glucuronide
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.92 - 2.8
4-methylumbelliferyl beta-D-mannopyranoside
0.92 - 2.8
4-methylumbelliferyl beta-D-mannoside
1.5 - 9.3
4-methylumbelliferyl beta-D-xylopyranoside
0.028 - 3.95
4-methylumbelliferyl beta-D-xyloside
1.2
4-methylumbelliferyl-beta-D-glucopyranoside
-
pH 5.5, 65ưC
0.8
4-methylumbelliferyl-beta-D-xylopyranoside
-
pH 5.5, 65ưC
427 - 462
4-nitrophenyl beta-D-fucopyranoside
5.16 - 772
4-nitrophenyl beta-D-fucoside
31 - 2374
4-nitrophenyl beta-D-galactopyranoside
0.047 - 4380
4-nitrophenyl beta-D-galactoside
4.2 - 940
4-nitrophenyl beta-D-glucopyranoside
0.018 - 542
4-nitrophenyl beta-D-glucoside
2.65
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
0.028 - 3.95
4-nitrophenyl beta-D-xyloside
448 - 503
4-nitrophenyl cellobioside
4.9 - 542
4-nitrophenyl-beta-D-glucopyranoside
4.3
4-nitrophenyl-beta-D-xylopyranoside
-
pH 5.5, 65ưC
1343 - 2257
ginsenoside Rb1
6.6
methyl beta-D-galactoside
release of methanol, pH 7.5, 80ưC
1.62
stevioside
at pH 6.0 and 80ưC
additional information
additional information
-
34
2-nitrophenyl beta-D-galactopyranoside

-
pH 6.5, 65ưC, mutant enzyme E206Q
295
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
366
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme delVal484-His489
416
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme His489
427
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme R488A
498
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
611
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, mutant enzyme R489A
1239
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, wild-type enzyme
1252
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1416
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
1662
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, immobilized enzyme
1686
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, enzyme immobilized on chitosan activated with glutaraldehyde
1689
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme
1713
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, recombinant enzyme
1713
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme
2437
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 60ưC
1300
2-nitrophenyl beta-D-galactoside

release of 2-nitrophenol, pH 7.5, 80ưC
2400
2-nitrophenyl beta-D-galactoside
-
pH 5.4, 75ưC
20.7
2-nitrophenyl beta-D-glucopyranoside

-
mutant E387G, pH 6.0, 65ưC
52.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 5.0, 65ưC
72.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.5, 65ưC, presence of 2 M formate
309
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65ưC
321.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 4.0, 65ưC
439.8
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65ưC, presence of 2 M formate
538
2-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
901.4
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 3.0, 65ưC
4.5
2-Nitrophenyl beta-D-glucoside

-
pH 6.5, 65ưC, mutant enzyme E206Q
252
2-Nitrophenyl beta-D-glucoside
-
pH 6.5, 65ưC, wild-type enzyme
18
4-methylumbelliferyl beta-D-fucopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
31
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
80
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
91
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
5.16
4-methylumbelliferyl beta-D-fucoside

pH 6.5, 45ưC, wild-type enzyme
9.43
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45ưC, mutant enzyme W433C
18
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
18
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, mutant enzyme E432C
31
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
31
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, mutant enzyme W433C
80
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
89
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, wild-type enzyme
5.4
4-methylumbelliferyl beta-D-galactopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
14
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
94
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
98
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
0.047
4-methylumbelliferyl beta-D-galactoside

pH 6.5, 45ưC, mutant enzyme E432C
0.08
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, mutant enzyme W433C
5.4
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
5.4
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, mutant enzyme E432C
7.2
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, wild-type enzyme
14.6
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
14.6
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, mutant enzyme W433C
98
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
98
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, wild-type enzyme
0.004
4-methylumbelliferyl beta-D-galacturonate

pH 6.5, 45ưC, mutant enzyme E432C
0.004
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45ưC, mutant enzyme W433C
0.81
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 80ưC, wild-type enzyme
5.1
4-methylumbelliferyl beta-D-glucopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
33
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
140
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
190
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.018
4-methylumbelliferyl beta-D-glucoside

pH 6.5, 45ưC, mutant enzyme E432C
2.72
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, mutant enzyme W433C
4.35
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, wild-type enzyme
5.1
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
5.1
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, mutant enzyme E432C
33
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
33
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, mutant enzyme W433C
140
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
140
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, wild-type enzyme
0.81
4-methylumbelliferyl beta-D-glucuronic acid

-
pH 6.5, 80ưC, wild-type enzyme
1.3
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80ưC, mutant enzyme M439C
0.92
4-methylumbelliferyl beta-D-mannopyranoside

-
pH 6.5, 80ưC, mutant enzyme W433C
1.8
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
2.3
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
2.8
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
0.92
4-methylumbelliferyl beta-D-mannoside

pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
0.92
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, mutant enzyme W433C
1.8
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
1.8
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, wild-type enzyme
2.65
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45ưC, wild-type enzyme
2.8
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
2.8
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, mutant enzyme E432C
1.5
4-methylumbelliferyl beta-D-xylopyranoside

-
pH 6.5, 80ưC, mutant enzyme W433C
2.8
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
3.8
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
9.3
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.028
4-methylumbelliferyl beta-D-xyloside

pH 6.5, 45ưC, mutant enzyme E432C
1.5
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
1.5
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, mutant enzyme W433C
2.8
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
2.8
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, mutant enzyme E432C
3.8
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
3.8
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, wild-type enzyme
3.95
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45ưC, wild-type enzyme
427
4-nitrophenyl beta-D-fucopyranoside

-
pH and temperature not specified in the publication, recombinant enzyme
460
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, native enzyme
462
4-nitrophenyl beta-D-fucopyranoside
-
pH 6.5, 60ưC
5.16
4-nitrophenyl beta-D-fucoside

pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
9.43
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
35
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 65ưC, mutant enzyme E206Q
263
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 65ưC, wild-type enzyme
503
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 75ưC, recombinant enzyme
772
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 75ưC, wild-type enzyme
31
4-nitrophenyl beta-D-galactopyranoside

-
pH 6.5, 65ưC, mutant enzyme E206Q
165
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 70ưC
275
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
445.6
4-nitrophenyl beta-D-galactopyranoside
-
pH 7.5, 75ưC
930
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1020
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, wild-type enzyme
1285
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75ưC, recombinant enzyme
1341
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
2374
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 60ưC
0.047
4-nitrophenyl beta-D-galactoside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.08
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
7.2
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
56
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 30ưC
143
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 40ưC
293
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 50ưC
648
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 60ưC
1000
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 65ưC
2288
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 75ưC
4380
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 85ưC
4.2
4-nitrophenyl beta-D-glucopyranoside

pH 6.5, 37ưC
45
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 70ưC
634
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
726
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, native enzyme
940
4-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 60ưC
0.018
4-nitrophenyl beta-D-glucoside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
2.72
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
4.35
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
4.4
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 65ưC, mutant enzyme E206Q
38.65
4-nitrophenyl beta-D-glucoside
at pH 6.0 and 80ưC
240
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 65ưC, wild-type enzyme
542
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 75ưC, recombinant enzyme
542
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 75ưC, wild-type enzyme
0.028
4-nitrophenyl beta-D-xyloside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
3.95
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
448
4-nitrophenyl cellobioside

-
pH 6.5, 75ưC, recombinant enzyme
503
4-nitrophenyl cellobioside
-
pH 6.5, 75ưC, wild-type enzyme
4.9
4-nitrophenyl-beta-D-glucopyranoside

-
pH 5.5, 65ưC
542
4-nitrophenyl-beta-D-glucopyranoside
-
-
23.2
cellobiose

pH 5.0, 70ưC
547
cellobiose
-
pH and temperature not specified in the publication, recombinant enzyme
628
cellobiose
-
pH and temperature not specified in the publication, soluble enzyme
644
cellobiose
-
pH 7.0, 75ưC, soluble enzyme
654
cellobiose
-
pH and temperature not specified in the publication, native enzyme
727
cellobiose
-
pH and temperature not specified in the publication, immobilized enzyme
746
cellobiose
-
pH 7.0, 75ưC, enzyme immobilized on chitosan activated with glutaraldehyde
1343
ginsenoside Rb1

mutant enzyme W361F, at pH 5.5 and 95ưC
2257
ginsenoside Rb1
wild type enzyme, at pH 5.5 and 95ưC
92
ginsenoside Rd

wild type enzyme, at pH 5.5 and 95ưC
1049
ginsenoside Rd
mutant enzyme W361F, at pH 5.5 and 95ưC
8.37
lactose

at pH 6.0 and 80ưC
121
lactose
pH 5.0, 70ưC
1500
lactose
release of D-glucose, pH 7.5, 80ưC
524
laminaribiose

-
pH and temperature not specified in the publication, native enzyme
573
laminaribiose
-
pH and temperature not specified in the publication, recombinant enzyme
274
oleuropein

-
pH 7.0, 60ưC, soluble enzyme
350.8
oleuropein
-
pH 7.0, 70ưC, soluble enzyme
additional information
additional information

-
kcat increases exponentially with temperature. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverĆ¢ĀĀBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated
-
additional information
additional information
-
kinetic constants for transglycosylation reactions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1617
2,4-dinitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
313.6 - 2137
2-nitrophenyl beta-D-galactopyranoside
1200 - 2700
2-nitrophenyl beta-D-galactoside
18.5 - 850
2-nitrophenyl beta-D-glucopyranoside
10.67
2-nitrophenyl beta-galactoside
at pH 6.0 and 80ưC
53 - 7300
4-methylumbelliferyl beta-D-fucopyranoside
0.053 - 7300
4-methylumbelliferyl beta-D-fucoside
6.3 - 1490
4-methylumbelliferyl beta-D-galactopyranoside
0.0063 - 1490
4-methylumbelliferyl beta-D-galactoside
0.0006 - 0.06
4-methylumbelliferyl beta-D-galacturonate
15 - 2900
4-methylumbelliferyl beta-D-glucopyranoside
0.015 - 2900
4-methylumbelliferyl beta-D-glucoside
0.6 - 0.92
4-methylumbelliferyl beta-D-glucuronic acid
0.6 - 5.1
4-methylumbelliferyl beta-D-glucuronide
3.2 - 53
4-methylumbelliferyl beta-D-mannopyranoside
0.001 - 76
4-methylumbelliferyl beta-D-mannoside
2.2 - 136
4-methylumbelliferyl beta-D-xylopyranoside
0.002 - 30
4-methylumbelliferyl beta-D-xyloside
941 - 4863
4-nitrophenyl beta-D-fucopyranoside
0.068 - 42.6
4-nitrophenyl beta-D-fucoside
105 - 2860
4-nitrophenyl beta-D-galactopyranoside
0.015 - 1400
4-nitrophenyl beta-D-galactoside
26.25 - 4700
4-nitrophenyl beta-D-glucopyranoside
0.036 - 29.8
4-nitrophenyl beta-D-glucoside
0.001 - 0.11
4-nitrophenyl beta-D-mannoside
0.002 - 0.77
4-nitrophenyl beta-D-xyloside
1764 - 11910
4-nitrophenyl-beta-D-glucopyranoside
697 - 3967
ginsenoside Rb1
0.034
methyl beta-D-galactoside
release of methanol, pH 7.5, 80ưC
0.094
stevioside
at pH 6.0 and 80ưC
additional information
additional information
-
313.6
2-nitrophenyl beta-D-galactopyranoside

-
pH 7.0, 75ưC, immobilized enzyme
337
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme, enzyme immobilized on chitosan activated with glutaraldehyde
412
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme
439
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75ưC, soluble enzyme
712
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
844
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
2137
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 60ưC
1200
2-nitrophenyl beta-D-galactoside

release of 2-nitrophenol, pH 7.5, 80ưC
2700
2-nitrophenyl beta-D-galactoside
-
pH 5.4, 75ưC
18.5
2-nitrophenyl beta-D-glucopyranoside

-
mutant E387G, pH 6.0, 65ưC
23.8
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 5.0, 65ưC
45
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.5, 65ưC, presence of 2 M formate
55
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 3.0, 65ưC
65.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 4.0, 65ưC
533
2-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65ưC, wild-type enzyme
533
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65ưC
850
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65ưC, presence of 2 M formate
53
4-methylumbelliferyl beta-D-fucopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
76
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
4000
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
7300
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
0.053
4-methylumbelliferyl beta-D-fucoside

pH 6.5, 80ưC, mutant enzyme E432C
0.068
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45ưC, mutant enzyme E432C
0.076
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, mutant enzyme W433C
0.506
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45ưC, mutant enzyme W433C
2.5
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
7.3
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80ưC, wild-type enzyme
42.6
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45ưC, wild-type enzyme
53
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
7300
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
6.3
4-methylumbelliferyl beta-D-galactopyranoside

-
pH 6.5, 80ưC, mutant enzyme W433C
11
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme E432C
1130
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
1490
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
0.0063
4-methylumbelliferyl beta-D-galactoside

pH 6.5, 80ưC, mutant enzyme W433C
0.011
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, mutant enzyme E432C
0.015
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, mutant enzyme W433C
0.058
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, mutant enzyme E432C
0.6
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
1.49
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80ưC, wild-type enzyme
11
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
12.7
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45ưC, wild-type enzyme
1490
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.0006
4-methylumbelliferyl beta-D-galacturonate

pH 6.5, 80ưC, wild-type enzyme
0.001
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45ưC, mutant enzyme E432C
0.0013
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45ưC, mutant enzyme W433C
0.06
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45ưC, wild-type enzyme
15
4-methylumbelliferyl beta-D-glucopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
20
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
2900
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
2900
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
0.015
4-methylumbelliferyl beta-D-glucoside

pH 6.5, 80ưC, mutant enzyme E432C
0.02
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, mutant enzyme W433C
0.036
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, mutant enzyme E432C
0.123
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, mutant enzyme W433C
2.9
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80ưC, wild-type enzyme
6.3
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
15
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
29.8
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45ưC, wild-type enzyme
2900
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
0.6
4-methylumbelliferyl beta-D-glucuronic acid

-
pH 6.5, 80ưC, wild-type enzyme
0.92
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80ưC, mutant enzyme M439C
0.6
4-methylumbelliferyl beta-D-glucuronide

pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
5.1
4-methylumbelliferyl beta-D-glucuronide
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
3.2
4-methylumbelliferyl beta-D-mannopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
5.1
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
50
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
53
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.001
4-methylumbelliferyl beta-D-mannoside

pH 6.5, 45ưC, mutant enzyme E432C
0.0032
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, mutant enzyme E432C
0.005
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45ưC, mutant enzyme W433C
0.0051
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, mutant enzyme W433C
0.05
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, wild-type enzyme
0.11
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45ưC, wild-type enzyme
3.2
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
50
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80ưC, wild-type enzyme
76
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
2.2
4-methylumbelliferyl beta-D-xylopyranoside

-
pH 6.5, 80ưC, mutant enzyme E432C
2.5
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, mutant enzyme W433C
30
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, wild-type enzyme
136
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80ưC, mutant enzyme M439C
0.002
4-methylumbelliferyl beta-D-xyloside

pH 6.5, 45ưC, mutant enzyme E432C
0.0022
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, mutant enzyme E432C
0.0025
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, mutant enzyme W433C
0.003
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45ưC, mutant enzyme W433C
0.03
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80ưC, wild-type enzyme
0.77
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45ưC, wild-type enzyme
2.2
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme E432C
20
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, mutant enzyme W433C
30
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80ưC, wild-type enzyme
941
4-nitrophenyl beta-D-fucopyranoside

-
pH and temperature not specified in the publication, recombinant enzyme
1151
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, native enzyme
4863
4-nitrophenyl beta-D-fucopyranoside
-
pH 6.5, 60ưC
0.068
4-nitrophenyl beta-D-fucoside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.506
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
42.6
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
105
4-nitrophenyl beta-D-galactopyranoside

pH 5.0, 70ưC
370
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
530.4
4-nitrophenyl beta-D-galactopyranoside
-
pH 7.5, 75ưC
576
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
2860
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 60ưC
0.015
4-nitrophenyl beta-D-galactoside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
0.058
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
12.7
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
1400
4-nitrophenyl beta-D-galactoside
-
pH 5.4, 75ưC
26.25
4-nitrophenyl beta-D-glucopyranoside

pH 6.5, 37ưC
375
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 70ưC
1058
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1633
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, native enzyme
4700
4-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 60ưC
0.036
4-nitrophenyl beta-D-glucoside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.123
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
18.95
4-nitrophenyl beta-D-glucoside
at pH 6.0 and 80ưC
29.8
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
0.001
4-nitrophenyl beta-D-mannoside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.005
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
0.11
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
0.002
4-nitrophenyl beta-D-xyloside

pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme E432C
0.003
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45ưC, mutant enzyme W433C
0.77
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45ưC, wild-type enzyme
1764
4-nitrophenyl-beta-D-glucopyranoside

-
pH 6.0, 60ưC, 1500 bar
3356
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 1000 bar
5811
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 750 bar
11130
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 500 bar
11910
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60ưC, 1 bar
1.72
cellobiose

pH 5.0, 70ưC
19.2
cellobiose
-
pH and temperature not specified in the publication, soluble enzyme
20.4
cellobiose
-
pH 7.0, 75ưC
25
cellobiose
-
pH and temperature not specified in the publication, recombinant enzyme
29
cellobiose
-
pH and temperature not specified in the publication, native enzyme
33.7
cellobiose
-
pH and temperature not specified in the publication, immobilized enzyme
37.3
cellobiose
-
pH 7.0, 75ưC, enzyme immobilized on chitosan activated with glutaraldehyde
697
ginsenoside Rb1

mutant enzyme W361F, at pH 5.5 and 95ưC
3967
ginsenoside Rb1
wild type enzyme, at pH 5.5 and 95ưC
178
ginsenoside Rd

wild type enzyme, at pH 5.5 and 95ưC
660
ginsenoside Rd
mutant enzyme W361F, at pH 5.5 and 95ưC
0.53
lactose

at pH 6.0 and 80ưC
3.19
lactose
pH 5.0, 70ưC
7.7
lactose
release of D-glucose, pH 7.5, 80ưC
407
laminaribiose

-
pH and temperature not specified in the publication, native enzyme
410
laminaribiose
-
pH and temperature not specified in the publication, recombinant enzyme
1.46
oleuropein

-
pH 7.0, 70ưC, soluble enzyme
1.62
oleuropein
-
pH 7.0, 60ưC, soluble enzyme
additional information
additional information

-
the efficiency (kcat/KM) increases with temperature and reaches its maximal efficiency at 65ưC. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverĆ¢ĀĀBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated
-
additional information
additional information
-
kinetic constants for transglycosylation reactions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0019
wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95ưC
0.0072
mutant enzyme W361F, with ginsenoside C-Mc as substrate, at pH 5.5 and 95ưC
0.012
mutant enzyme W361F, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95ưC
0.0126
wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95ưC
0.0154
wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95ưC
0.0201
wild type enzyme, with ginsenoside Rc as substrate, at pH 5.5 and 95ưC
0.0233
mutant enzyme W361F, with ginsenoside Rc as substrate, at pH 5.5 and 95ưC
0.0355
mutant enzyme W361F, with naringin as substrate, at pH 5.5 and 95ưC
0.0391
wild type enzyme, with hesperidin as substrate, at pH 5.5 and 95ưC
0.0567
mutant enzyme L213A, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95ưC
0.0661
wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95ưC
0.0802
mutant enzyme L213A, with ginsenoside Rd as substrate, at pH 5.5 and 95ưC
0.146
mutant enzyme L213A, with ginsenoside C-Mc as substrate, at pH 5.5 and 95ưC
0.211
wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95ưC
0.233
wild type enzyme, with naringin as substrate, at pH 5.5 and 95ưC
0.288
mutant enzyme W361F, with hesperidin as substrate, at pH 5.5 and 95ưC
0.338
wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95ưC
0.52
wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95ưC
0.54
mutant enzyme L213A, with ginsenoside Rb2 as substrate, at pH 5.5 and 95ưC
0.58
pH 6.5, 75ưC, enzyme immobilized on XAD-4
0.59
pH 6.5, 75ưC, enzyme immobilized on XAD-16
1.18
mutant enzyme L213A, with ginsenoside C-Y as substrate, at pH 5.5 and 95ưC
1.2
-
mutant E387G, substrate cellobiose, pH 3.0, 65ưC
1.347
mutant enzyme W361F, with ginsenoside C-Y as substrate, at pH 5.5 and 95ưC
1.415
mutant enzyme W361F, with ginsenoside Rd as substrate, at pH 5.5 and 95ưC
1.643
wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95ưC
1.71
pH 6.5, 75ưC, lyophilized enzyme
1.74
wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95ưC
127
-
mutant E387G, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65ưC
13.13
wild type enzyme, with ginsenoside F2 as substrate, at pH 5.5 and 95ưC
13.227
mutant enzyme W361F, with ginsenoside F2 as substrate, at pH 5.5 and 95ưC
13.65
mutant enzyme L213A, with ginsenoside Rb1 as substrate, at pH 5.5 and 95ưC
15
-
mutant E387G, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65ưC
150
-
wild-type, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65ưC
19.1
-
wild-type, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65ưC
196
-
wild-type, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65ưC
2.031
mutant enzyme W361F, with ginsenoside C-O as substrate, at pH 5.5 and 95ưC
2.22
pH 6.5, 75ưC, addition of 1% (w/v) SDS
2.5
-
mutant E387G, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65ưC
2.7
-
mutant E387G, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65ưC
219
-
wild-type, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65ưC
24.1
wild type enzyme, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95ưC
253
-
wild-type, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65ưC
3.213
mutant enzyme W361F, with ginsenoside Rb1 as substrate, at pH 5.5 and 95ưC
3.53
pH 6.5, 75ưC, enzyme immobilized on Celite
33.4
mutant enzyme L213A, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95ưC
4.05
mutant enzyme L213A, with ginsenoside C-O as substrate, at pH 5.5 and 95ưC
4.24
wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95ưC
4.388
wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95ưC
5.082
wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95ưC
6.354
wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95ưC
61.9
-
wild-type, substrate cellobiose, pH 3.0, 65ưC
63.3
-
mutant E387G, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65ưC
8.56
wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95ưC
9.077
mutant enzyme W361F, with ginsenoside Rb2 as substrate, at pH 5.5 and 95ưC
166

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