Literature summary for 3.2.1.B26 extracted from

Cobucci-Ponzano, B.; Aurilia, V.; Riccio, G.; Henrissat, B.; Coutinho, P.M.; Strazzulli, A.; Padula, A.; Corsaro, M.M.; Pieretti, G.; Pocsfalvi, G.; Fiume, I.; Cannio, R.; Rossi, M.; Moracci, M.
A new archaeal beta-glycosidase from Sulfolobus solfataricus: seeding a novel retaining beta-glycan-specific glycoside hydrolase family along with the human non-lysosomal glucosylceramidase GBA2 (2010), J. Biol. Chem., 285, 20691-20703.

Search for more literature for 3.2.1.B26

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
D406G	inactive mutant	Saccharolobus solfataricus
D458G	inactive mutant	Saccharolobus solfataricus
D462G	inactive mutant. Addition of 1 mM NaN3 rescues enzymatic activity using 2-nitrophenyl-beta-D-glucopyranoside as substrate. Activity is 7fold lower compared to wild-type	Saccharolobus solfataricus
E335G	inactive mutant	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
2,4-dinitrophenyl-beta-2-deoxy-2-fluoro-D-glucopyranoside	-	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
2.6	-	4-methylumbelliferyl-beta-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
13	-	2-Nitrophenyl-beta-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
25	-	4-nitrophenyl-beta-D-xylopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
54	-	4-nitrophenyl-beta-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75910	-	MALDI-TOF	Saccharolobus solfataricus
76000	-	gel filtration	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus	2-nitrophenol + D-glucopyranose	-	?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-glucopyranose	-	?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-xylopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + beta-D-xylopyranose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 76000, gel filtration	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
beta-glucosidase	-	Saccharolobus solfataricus
sso1353	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.8	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
1.2	-	4-methylumbelliferyl-beta-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
4.3	-	4-nitrophenyl-beta-D-xylopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
4.7	-	2-Nitrophenyl-beta-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus
4.9	-	4-nitrophenyl-beta-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	-	assay at	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
5.5	-	2,4-dinitrophenyl-beta-2-deoxy-2-fluoro-D-glucopyranoside	pH 5.5, 65°C	Saccharolobus solfataricus

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Release 2023.1 (January 2023)