EC Number   |
Inhibitors |
Structure |
|---|
   3.2.1.B26 | (5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol |
inhibits activity with 4-nitrophenyl beta-D-galactopyranoside |
   |
| 3.2.1.B26 | 1,4-D-galactonolactone |
0.1 M, 98% inhibition of beta-galactoside hydrolysis, 90% inhibition of beta-glucoside hydrolysis |
|
| 3.2.1.B26 | 1,4-dioxane |
10% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-fucopyranoside |
|
| 3.2.1.B26 | 1,5-D-gluconolactone |
0.1 M, complete inhibition of beta-galactoside hydrolysis and beta-glucoside hydrolysis |
|
| 3.2.1.B26 | 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucoside |
a mechanism-based inhibitor. 102fold molar excess, 80% inhibition of wild-type enzyme after 30 min and complete after overnight incubation. The E387G mutant enzyme is insensitive to the inhibitor |
|
| 3.2.1.B26 | 2,4-dinitrophenyl-beta-2-deoxy-2-fluoro-D-glucopyranoside |
- |
|
| 3.2.1.B26 | 2-phenethyl glucoimidazole |
- |
|
| 3.2.1.B26 | 4-nitrophenyl-beta-D-galactopyranoside |
at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate |
|
| 3.2.1.B26 | Ba2+ |
the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ |
|
| 3.2.1.B26 | butan-2-one |
10-20 (v/v) |
|
