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Literature summary for 3.2.1.B26 extracted from

  • Perugino, G.; Trincone, A.; Giordano, A.; van der Oost, J.; Kaper, T.; Rossi, M.; Moracci, M.
    Activity of hyperthermophilic glycosynthases is significantly enhanced at acidic pH (2003), Biochemistry, 42, 8484-8493.
    View publication on PubMed
Search for more literature for 3.2.1.B26

Protein Variants

Protein Variants Comment Organism
E386G mutation in nucleophile residue E387, mutation completely abolishes activity under statndard conditions. The addition of 2 M sodium formate as an external nucleophile leads to the recovery of 8.40% activity with accumulation of oligosaccharides. At pH 3.0 and low concentrations of sodium formate buffer, the hyperthermophilic glycosynthase shows kcat values similar to those of the wild-type and 17fold higher than those observed at the usual reactivation conditions in 2 M sodium formate at pH 6.5 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 2-nitrophenyl beta-D-glucopyranoside wild-type, pH 6.5, 65°C, presence of 2 M formate Saccharolobus solfataricus
1.01
-
 2-nitrophenyl beta-D-glucopyranoside wild-type, pH 6.5, 65°C Saccharolobus solfataricus
1.1
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 6.0, 65°C Saccharolobus solfataricus
1.17
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 6.5, 65°C, presence of 2 M formate Saccharolobus solfataricus
2.2
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 5.0, 65°C Saccharolobus solfataricus
4.9
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 4.0, 65°C Saccharolobus solfataricus
16.4
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 3.0, 65°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 mutant E387G, substrate cellobiose, pH 3.0, 65°C Saccharolobus solfataricus
2.5
-
 mutant E387G, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
2.7
-
 mutant E387G, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
15
-
 mutant E387G, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
19.1
-
 wild-type, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
61.9
-
 wild-type, substrate cellobiose, pH 3.0, 65°C Saccharolobus solfataricus
63.3
-
 mutant E387G, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
127
-
 mutant E387G, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
150
-
 wild-type, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
196
-
 wild-type, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
219
-
 wild-type, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65°C Saccharolobus solfataricus
253
-
 wild-type, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 2,4-dinitrophenol + D-glucopyranose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + D-galactopyranose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + D-glucopyranose
-
 ?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + D-xylopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
 ?
cellobiose + H2O
-
 Saccharolobus solfataricus 2 D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
beta-Gly
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.7
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 6.0, 65°C Saccharolobus solfataricus
52.9
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 5.0, 65°C Saccharolobus solfataricus
72.9
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 6.5, 65°C, presence of 2 M formate Saccharolobus solfataricus
309
-
 2-nitrophenyl beta-D-glucopyranoside wild-type, pH 6.5, 65°C Saccharolobus solfataricus
321.9
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 4.0, 65°C Saccharolobus solfataricus
439.8
-
 2-nitrophenyl beta-D-glucopyranoside wild-type, pH 6.5, 65°C, presence of 2 M formate Saccharolobus solfataricus
901.4
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 3.0, 65°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18.5
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 6.0, 65°C Saccharolobus solfataricus
23.8
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 5.0, 65°C Saccharolobus solfataricus
45
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 6.5, 65°C, presence of 2 M formate Saccharolobus solfataricus
55
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 3.0, 65°C Saccharolobus solfataricus
65.9
-
 2-nitrophenyl beta-D-glucopyranoside mutant E387G, pH 4.0, 65°C Saccharolobus solfataricus
533
-
 2-nitrophenyl beta-D-glucopyranoside wild-type, pH 6.5, 65°C Saccharolobus solfataricus
850
-
 2-nitrophenyl beta-D-glucopyranoside wild-type, pH 6.5, 65°C, presence of 2 M formate Saccharolobus solfataricus