Any feedback?
Literature summary for 3.2.1.B26 extracted from
- Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable beta-glycosidases (1999), Biotechnol. Bioeng., 64, 322-332.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | activated up to 2fold in the presence of D-glucose with respect to the maximum rate of glycosidic bond cleavage, measured with 2-nitrophenyl beta-D-galactoside as the substrate | Saccharolobus solfataricus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme suitable for hydrolysis of lactose at temperatures at 70-80°C | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactose | measured with lactose as the substrate | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 92 | - |
lactose | pH 5.5, 80°C | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| lactose + H2O | - |
Saccharolobus solfataricus | D-glucose + D-galactose | - |
? | |
| additional information | the transfer of the galactosyl group from lactose to acceptors such as lactose or D-glucose rather than water is significant for both enzymes and depends on the initial lactose concentration as well as the time-dependent substrate/product ratio during batchwise lactose conversion | Saccharolobus solfataricus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SsbetaGly | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability is not affected by the presence of Mg2+ | Saccharolobus solfataricus |
| 70 | - |
t1/2: 10 d | Saccharolobus solfataricus |
| 80 | - |
t1/2: about 5 d | Saccharolobus solfataricus |
| 90 | - |
t1/2: 0.7 d | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 366 | - |
D-galactose | pH 5.5, 80°C | Saccharolobus solfataricus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
