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Literature summary for 3.2.1.B26 extracted from

  • Hansson, T.; Adlercreutz, P.
    Enzymatic synthesis of hexyl glycosides from lactose at low water activity and high temperature using hyperthermostable beta-glycosidases (2002), Biocatal. Biotransform., 20, 167-178.
No PubMed abstract available
Search for more literature for 3.2.1.B26

Application

Application Comment Organism
synthesis optimization of hexyl-beta-D-glycoside synthesis from lactose in hexanol at low water activity and high temperature. Compared to other beta-glycosidases in lactose conversion into alkyl glycoside, the enzyme shows high activity in a hexanol one-phase system and synthesized high yields of both hexyl-beta-D-galactoside and hexyl-beta-D-glucoside. Using 32 g/l lactose (93 mM), the enzyme synthesizes yields of 41% galactoside (38.1 mM) and 29% glucoside (27.0 mM) Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P22498
-
-
Saccharolobus solfataricus P2 P22498
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.58
-
 pH 6.5, 75°C, enzyme immobilized on XAD-4 Saccharolobus solfataricus
0.59
-
 pH 6.5, 75°C, enzyme immobilized on XAD-16 Saccharolobus solfataricus
1.71
-
 pH 6.5, 75°C, lyophilized enzyme Saccharolobus solfataricus
2.22
-
 pH 6.5, 75°C, addition of 1% (w/v) SDS Saccharolobus solfataricus
3.53
-
 pH 6.5, 75°C, enzyme immobilized on Celite Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactoside LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactoside LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucoside LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-glucose
-
 ?
additional information the enzyme catalyzes the conversion of lactose to hexyl-beta-D-galactoside in hexanol (transgalactosylation reaction) Saccharolobus solfataricus ?
-
 ?
additional information the enzyme catalyzes the conversion of lactose to hexyl-beta-D-galactoside in hexanol (transgalactosylation reaction) Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

Synonyms Comment Organism
LACS
-
 Saccharolobus solfataricus
SSO3019 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharolobus solfataricus