Literature summary for 3.2.1.B26 extracted from

Petzelbauer, I.; Zeleny, R.; Reiter, A.; Kulbe, K.D.; Nidetzky, B.
Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose: II. Oligosaccharide formation by two thermostable beta-glycosidases (2000), Biotechnol. Bioeng., 69, 140-149.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	during lactose conversion at 70°C galactosyl transfer to acceptors other than water competes efficiently with complete hydrolysis of substrate. This process leads to transient formation of a range of new products, mainly disaccharides and trisaccharides, and shows a marked dependence on initial substrate concentration and lactose conversion. The molar ratio of trisaccharides to disaccharides is maximal at an early stage of reaction and decreases directly proportional to increasing substrate conversion. The major transgalactosylation products of SsbetaGl are beta-D-Galp-(1->3)-Glc and beta-D-Galp-(1->6)-Glc, and beta-D-Galp-(1->3)-lactose and beta-D-Galp-(1-->6)-lactose. The enzyme accumulates beta(1-->6)-linked glycosides, particularly allolactose, at a late stage of reaction	Saccharolobus solfataricus	?	-	?

Synonyms

Synonyms	Comment	Organism
SsbetaGly	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)