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Literature summary for 3.2.1.B26 extracted from

  • Pouwels, J.; Moracci, M.; Cobucci-Ponzano, B.; Perugino, G.; van der Oost, J.; Kaper, T.; Lebbink, J.H.; de Vos, W.M.; Ciaramella, M.; Rossi, M.
    Activity and stability of hyperthermophilic enzymes: a comparative study on two archaeal beta-glycosidases (2000), Extremophiles, 4, 157-164.
    View publication on PubMed
Search for more literature for 3.2.1.B26

Activating Compound

Activating Compound Comment Organism Structure
SDS 50°C, 30 min, 0.03%, slight activation Saccharolobus solfataricus

General Stability

General Stability Organism
chaotropes (I-, ClO4-, NO3- and Cs+) have a strong destabilising effect on the protein by perturbing hydrophobic interactions Saccharolobus solfataricus
denaturing effect of NaCl and Na2SO4 Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
SDS 50°C, 30 min, 0.3% SDS, 40% loss of activity Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM increases with temperature: the increase is moderate at lower temperatures (from 30°C to 65°C) and more evident between 65°C and 85°C. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in Lineweaver–Burk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated Saccharolobus solfataricus
0.59
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 30°C Saccharolobus solfataricus
0.72
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 60°C Saccharolobus solfataricus
0.77
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 65°C Saccharolobus solfataricus
0.82
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 40°C Saccharolobus solfataricus
0.87
-
 2-nitrophenyl beta-D-galactoside pH 5.4, 75°C Saccharolobus solfataricus
1.09
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 50°C Saccharolobus solfataricus
1.61
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 75°C Saccharolobus solfataricus
3.16
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 85°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Sbetagly
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 inactivation is coupled to irreversible aggregation Saccharolobus solfataricus
75
-
 30 min, 90% residual activity Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kcat increases exponentially with temperature. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in Lineweaver–Burk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated Saccharolobus solfataricus
56
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 30°C Saccharolobus solfataricus
143
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 40°C Saccharolobus solfataricus
293
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 50°C Saccharolobus solfataricus
648
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 60°C Saccharolobus solfataricus
1000
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 65°C Saccharolobus solfataricus
2288
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 75°C Saccharolobus solfataricus
2400
-
 2-nitrophenyl beta-D-galactoside pH 5.4, 75°C Saccharolobus solfataricus
4380
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 85°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6.5 at 65°C Saccharolobus solfataricus
5 5.5 at 75°C Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 activity dependence on pH at 65°C and 75°C on 5 mM 4-nitrophenyl beta-D-galactoside Saccharolobus solfataricus
4 7 pH 4.0: about 60% of maximal activity at 75°C, pH 7.0: about 50% of maximal activity at 75°C Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.4
-
 75°C, half-life is less than 5 min, irreversible aggregation Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information the efficiency (kcat/KM) increases with temperature and reaches its maximal efficiency at 65°C. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in Lineweaver–Burk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated Saccharolobus solfataricus
1400
-
 4-nitrophenyl beta-D-galactoside pH 5.4, 75°C Saccharolobus solfataricus
2700
-
 2-nitrophenyl beta-D-galactoside pH 5.4, 75°C Saccharolobus solfataricus