Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl-beta-D-galactopyranoside | at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate | Saccharolobus solfataricus | |
additional information | this enzyme appears to be activated by pressure between atmospheric pressure and 2.5 kbar with a maximal activity at 1.25 kbar. However, this enzyme still displayed the best catalytic efficiency at atmospheric pressure because of a Km value drastically increased by pressure | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
highly barostable enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl-beta-D-galactopyranoside | at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 1 bar | Saccharolobus solfataricus | |
0.16 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 500 bar | Saccharolobus solfataricus | |
0.41 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 750 bar | Saccharolobus solfataricus | |
0.55 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 1000 bar | Saccharolobus solfataricus | |
0.86 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 1500 bar | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-glucopyranose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 120 | 80°C: about 40% of maximal activity, 110°C: 85% of maximal activity, 120°C: about 25% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
after a storage greater than 40 h at atmospheric pressure, the residual activity decreases | Saccharolobus solfataricus |
60 | - |
maintains a half-life of 91 h at 60°C and 2.5 kbar | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1764 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 1500 bar | Saccharolobus solfataricus | |
3356 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 1000 bar | Saccharolobus solfataricus | |
5811 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 750 bar | Saccharolobus solfataricus | |
11130 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 500 bar | Saccharolobus solfataricus | |
11910 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C, 1 bar | Saccharolobus solfataricus |