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Literature summary for 3.2.1.B26 extracted from
- Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. IV. Immobilization of two thermostable beta-glycosidases and optimization of a packed-bed reactor for lactose conversion (2002), Biotechnol. Bioeng., 77, 619-631.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the immobilized enzyme is useful for the hydrolysis of lactose in whey or milk by using a packed-bed enzyme reactor operated at 70°C | Saccharolobus solfataricus |
| synthesis | the immobilized enzyme is useful for the production of galactooligosaccharides by using a packed-bed enzyme reactor operated at 70°C | Saccharolobus solfataricus |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme can be immobilized with little loss of enzyme activity and catalytic efficiency by covalent coupling of the proteins via the surface amino groups to insoluble carriers | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 44 | - |
lactose | pH 5.5, 70°C, enzyme immobilized on Eupergit C | Saccharolobus solfataricus |  |
| 63 | - |
lactose | pH 5.5, 70°C, soluble enzyme | Saccharolobus solfataricus | |
| 70 | - |
lactose | pH 5.5, 70°C, enzyme immobilized on chitosan | Saccharolobus solfataricus | |
| 131 | - |
lactose | pH 5.5, 70°C, enzyme immobilized on controlled pore glass | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Saccharolobus solfataricus | D-glucose + D-galactose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SsbetaGly | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 5.5, immobilized enzyme, half-life: 10 days | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | soluble enzyme and immobilized enzymes | Saccharolobus solfataricus |
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