Literature summary for 3.2.1.B26 extracted from

D'Auria, S.; Pellino, F.; La Cara, F.; Barone, R.; Rossi, M.; Nucci, R.
Immobilization on chitosan of a thermophilic beta-glycosidase expressed in Saccharomyces cerevisiae (1996), Appl. Biochem. Biotechnol., 61, 157-166.

Search for more literature for 3.2.1.B26

Application

Application	Comment	Organism
synthesis	development of a continuous cellobiose hydrolysis system for glucose production with a high degree of conversion by the enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	soluble and immobilized enzyme show no inhibition by glucose	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic constant values for soluble enzyme and for enzyme immobilized on chitosan activated with glutaraldehyde are comparable	Saccharolobus solfataricus
3.9	-	2-nitrophenyl beta-D-galactopyranoside	pH 7.0, 75°C, soluble enzyme	Saccharolobus solfataricus
5	-	2-nitrophenyl beta-D-galactopyranoside	pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus
20	-	cellobiose	pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus
31.5	-	cellobiose	pH 7.0, 75°C, soluble enzyme	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Storage Stability

Storage Stability	Organism
4°C, enzyme immobilized on chitosan activated with glutaraldehyde, stable for 2 months, 20% loss of activity after 4 months	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O	-	Saccharolobus solfataricus	2-nitrophenol + beta-D-galactopyranose	-	?
cellobiose + H2O	-	Saccharolobus solfataricus	D-glucose + D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
Sbetagly	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	assay at	Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	80	enzyme immobilized on chitosan activated with glutaraldehyde shows an increase in the temperature range 30°C-80°C in 50 mM sodium phosphate buffer, pH 7.0	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
75	-	5 h, enzyme immobilized on chitosan activated with glutaraldehyde, 15% loss of activity	Saccharolobus solfataricus
85	-	5 h, enzyme immobilized on chitosan activated with glutaraldehyde, 25% loss of activity	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
644	-	cellobiose	pH 7.0, 75°C, soluble enzyme	Saccharolobus solfataricus
746	-	cellobiose	pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus
1686	-	2-nitrophenyl beta-D-galactopyranoside	pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus
1713	-	2-nitrophenyl beta-D-galactopyranoside	pH 7.0, 75°C, soluble enzyme	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	7.5	pH 6.0: about 45% of maximal activity, pH 7.0: optimum, pH 7.5: less than 10% of maximal activity	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
20.4	-	cellobiose	pH 7.0, 75°C	Saccharolobus solfataricus
37.3	-	cellobiose	pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus
337	-	2-nitrophenyl beta-D-galactopyranoside	pH 7.0, 75°C, soluble enzyme, enzyme immobilized on chitosan activated with glutaraldehyde	Saccharolobus solfataricus
439	-	2-nitrophenyl beta-D-galactopyranoside	pH 7.0, 75°C, soluble enzyme	Saccharolobus solfataricus

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Release 2023.1 (January 2023)