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Literature summary for 3.2.1.B26 extracted from

  • Kaper, T.; Brouns, S.J.; Geerling, A.C.; De Vos, W.M.; Van der Oost, J.
    DNA family shuffling of hyperthermostable beta-glycosidases (2002), Biochem. J., 368, 461-470.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.B26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information generation of functional glycoside hydrolase hybrids by shuffling of the genes coding for Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS. Generating of thermostable hybrid beta-glycosidases and isolating high-performance variants Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol inhibits activity with 4-nitrophenyl beta-D-galactopyranoside Saccharolobus solfataricus
D-glucose inhibits activity with 4-nitrophenyl beta-D-galactopyranoside Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 70°C Saccharolobus solfataricus
1.57
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 70°C Saccharolobus solfataricus
13.5
-
 cellobiose pH 5.0, 70°C Saccharolobus solfataricus
37.9
-
 lactose pH 5.0, 70°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P22498
-
-
Saccharolobus solfataricus P2 P22498
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + D-glucopyranose
-
 ?
cellobiose + H2O
-
 Saccharolobus solfataricus D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Saccharolobus solfataricus P2 D-glucose + D-glucose
-
 ?
lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Saccharolobus solfataricus P2 D-glucose + D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
LACS
-
 Saccharolobus solfataricus
SSO3019 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus
95
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1.5 h, no residual activity Saccharolobus solfataricus
92
-
 half-life: less than 3 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.2
-
 cellobiose pH 5.0, 70°C Saccharolobus solfataricus
45
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 70°C Saccharolobus solfataricus
121
-
 lactose pH 5.0, 70°C Saccharolobus solfataricus
165
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 70°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000084
-
 (5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol pH 5.0, 70°C, substrate: 4-nitrophenyl beta-D-galactopyranoside Saccharolobus solfataricus
46
-
 D-glucose pH 5.0, 70°C, substrate: 4-nitrophenyl beta-D-galactopyranoside Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.72
-
 cellobiose pH 5.0, 70°C Saccharolobus solfataricus
3.19
-
 lactose pH 5.0, 70°C Saccharolobus solfataricus
105
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 70°C Saccharolobus solfataricus
375
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 70°C Saccharolobus solfataricus