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Literature summary for 3.2.1.B26 extracted from

  • Nguyen, T.T.; Kim, S.B.; Kim, N.M.; Kang, C.; Chung, B.; Park, J.S.; Kim, D.
    Production of steviol from steviol glucosides using beta-glycosidase from Sulfolobus solfataricus (2016), Enzyme Microb. Technol., 93-94, 157-165 .
    View publication on PubMed
Search for more literature for 3.2.1.B26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta BL21(DE3)pLyS cells Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive inhibitor Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.04
-
 4-nitrophenyl beta-D-glucoside at pH 6.0 and 80°C Saccharolobus solfataricus
2.25
-
 2-nitrophenyl beta-galactoside at pH 6.0 and 80°C Saccharolobus solfataricus
15.82
-
 lactose at pH 6.0 and 80°C Saccharolobus solfataricus
17.21
-
 stevioside at pH 6.0 and 80°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P22498
-
-
Saccharolobus solfataricus DSM 1617 P22498
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-galactoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + D-galactose
-
 ?
2-nitrophenyl beta-galactoside + H2O
-
 Saccharolobus solfataricus DSM 1617 2-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O highest activity Saccharolobus solfataricus 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O highest activity Saccharolobus solfataricus DSM 1617 4-nitrophenol + D-glucose
-
 ?
lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Saccharolobus solfataricus DSM 1617 D-glucose + D-galactose
-
 ?
rebaudioside A + H2O negligible activity Saccharolobus solfataricus steviol + D-glucose
-
 ?
rebaudioside A + H2O negligible activity Saccharolobus solfataricus DSM 1617 steviol + D-glucose
-
 ?
rubusoside + H2O 44.2% yield Saccharolobus solfataricus steviol + D-glucose
-
 ?
rubusoside + H2O 44.2% yield Saccharolobus solfataricus DSM 1617 steviol + D-glucose
-
 ?
stevioside + H2O
-
 Saccharolobus solfataricus steviol + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
beta-glycosidase
-
 Saccharolobus solfataricus
bgly
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.62
-
 stevioside at pH 6.0 and 80°C Saccharolobus solfataricus
8.37
-
 lactose at pH 6.0 and 80°C Saccharolobus solfataricus
24
-
 2-nitrophenyl beta-galactoside at pH 6.0 and 80°C Saccharolobus solfataricus
38.65
-
 4-nitrophenyl beta-D-glucoside at pH 6.0 and 80°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.094
-
 stevioside at pH 6.0 and 80°C Saccharolobus solfataricus
0.53
-
 lactose at pH 6.0 and 80°C Saccharolobus solfataricus
10.67
-
 2-nitrophenyl beta-galactoside at pH 6.0 and 80°C Saccharolobus solfataricus
18.95
-
 4-nitrophenyl beta-D-glucoside at pH 6.0 and 80°C Saccharolobus solfataricus