Literature summary for 3.2.1.B26 extracted from

Choi, J.H.; Shin, K.C.; Oh, D.K.
An L213A variant of beta-glycosidase from Sulfolobus solfataricus with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to compound K (2018), PLoS ONE, 13, e0191018 .

Search for more literature for 3.2.1.B26

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
G217A	the mutant converts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
G217N	the mutantconverts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
H342A	the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol)	Saccharolobus solfataricus
H342E	inactive	Saccharolobus solfataricus
K219R	the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol)	Saccharolobus solfataricus
L213A	the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol). The variant enzyme converts ginsenosides Rc and C-Mc into C-K with molar conversions of 97%, which are 1.5 and 2fold higher, respectively, than those of the wild type enzyme	Saccharolobus solfataricus
L213D	the mutant converts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
L213E	the mutant converts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
L213G	the mutant converts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
L213H	the mutant converts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
L213Q	the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol)	Saccharolobus solfataricus
L213R	inactive	Saccharolobus solfataricus
L213S	the mutant converts ginsenoside Rc to ginsenoside Rd	Saccharolobus solfataricus
L213W	the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol)	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P22498	-	-
Saccharolobus solfataricus DSM 1617	P22498	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0126	-	wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.0567	-	mutant enzyme L213A, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.0661	-	wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.0802	-	mutant enzyme L213A, with ginsenoside Rd as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.146	-	mutant enzyme L213A, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.211	-	wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.52	-	wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
0.54	-	mutant enzyme L213A, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
1.18	-	mutant enzyme L213A, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
1.74	-	wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
4.05	-	mutant enzyme L213A, with ginsenoside C-O as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
4.24	-	wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
8.56	-	wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
13.65	-	mutant enzyme L213A, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
24.1	-	wild type enzyme, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus
33.4	-	mutant enzyme L213A, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95°C	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ginsenoside C-Mc + H2O	-	Saccharolobus solfataricus	?	-	?
ginsenoside C-Mc + H2O	-	Saccharolobus solfataricus DSM 1617	?	-	?
ginsenoside C-Mc1 + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + D-glucose + L-arabinofuranose	-	?
ginsenoside C-O + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + D-glucose + L-arabinopyranose	-	?
ginsenoside C-Y + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + L-arabinopyranose	-	?
ginsenoside F2 + H2O	highest activity	Saccharolobus solfataricus	ginsenoside C-K + D-glucose	-	?
ginsenoside Rb1 + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + D-glucose	-	?
ginsenoside Rb2 + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + D-glucose + L-arabinopyranose	-	?
ginsenoside Rc + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + D-glucose + L-arabinofuranose	-	?
ginsenoside Rd + H2O	-	Saccharolobus solfataricus	ginsenoside C-K + D-glucose	-	?
additional information	no activity with alpha-L-arabinofuranoside linked to ginsenoside Rc	Saccharolobus solfataricus	?	-	?
additional information	the specific hydrolytic activity for the wild type enzyme follows the order ginsenoside F2 > Rb1 > C-O > C-Y > Rb2 > Rd > C-Mc> C-Mc1	Saccharolobus solfataricus	?	-	?
additional information	no activity with alpha-L-arabinofuranoside linked to ginsenoside Rc	Saccharolobus solfataricus DSM 1617	?	-	?
additional information	the specific hydrolytic activity for the wild type enzyme follows the order ginsenoside F2 > Rb1 > C-O > C-Y > Rb2 > Rd > C-Mc> C-Mc1	Saccharolobus solfataricus DSM 1617	?	-	?

Synonyms

Synonyms	Comment	Organism
beta-glycosidase	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)