Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | maximal increase in enzyme activity at solvent concentrations of 15% (v/v) with 4-nitrophenyl beta-D-fucopyranoside as substrate | Saccharolobus solfataricus | |
butan-2-one | maximum increase in enzyme activity (1.5fold compared to activity in buffer) is measured at 5% (v/v) solvent concentrations with 4-nitrophenyl beta-D-fucopyranoside as substrate | Saccharolobus solfataricus | |
methyl acetate | 5% (v/v), increases activity with 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside or 4-nitrophenyl beta-D-glucopyranoside | Saccharolobus solfataricus | |
methyl acetate | highest activation at solvent concentrations of 5% (v/v) with 4-nitrophenyl beta-D-fucopyranoside as substrate | Saccharolobus solfataricus | |
tetrahydrofuran | 5% (v/v), increases activity with 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-dioxane | 10% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-fucopyranoside | Saccharolobus solfataricus | |
butan-2-one | 10-20 (v/v) | Saccharolobus solfataricus | |
D-gluconic acid lactone | strong competitive inhibitor | Saccharolobus solfataricus | |
tetrahydrofuran | 5% (v/v), decreases activity with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-fucopyranoside | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.83 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
1.14 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
72 h, stable | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
462 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
940 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
2374 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
2437 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
D-gluconic acid lactone | pH 6.5, 60°C | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2137 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
2860 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
4700 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus | |
4863 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.5, 60°C | Saccharolobus solfataricus |