Literature summary for 3.2.1.B26 extracted from

Hancock, S.M.; Corbett, K.; Fordham-Skelton, A.P.; Gatehouse, J.A.; Davis, B.G.
Developing promiscuous glycosidases for glycoside synthesis: residues W433 and E432 in Sulfolobus solfataricus beta-glycosidase are important glucoside- and galactoside-specificity determinants (2005), Chembiochem, 6, 866-875.

Search for more literature for 3.2.1.B26

Application

Application	Comment	Organism
synthesis	formation of mannosides and xylosides by transglycosylation	Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutated sequences are expressed in Escherichia coli with a His7-tag to allow one-step chromatographic purification	Saccharolobus solfataricus
wild-type enzyme and mutant enzymes E432C and W433C are expressed in Escherichia coli with a His7-tag to allow one-step chromatographic purification	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
E432C	kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 200fold, kcat/KM for 4-methylumbelliferyl beta-D-galactoside is reduced 130fold, kcat/KM for 4-methylumbelliferyl beta-D-xyloside is reduced 1.5fold, kcat/KM for 4-methylumbelliferyl beta-D-fucoside is reduced 2900fold, kcat/KM for 4-methylumbelliferyl beta-D-mannoside is ibcreased 1.5fold	Saccharolobus solfataricus
E432C	the kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 200fold, and 4-methylumbelliferyl beta-D-galactoside is reduced 130fold. Although the activities with 4-methylumbelliferyl beta-D-mannoside and 4-methylumbelliferyl beta-D-xyloside are also reduced in the mutant, this is to a much smaller extent. The mutant enzyme shows remarkably broader specificity than wild-type enzyme	Saccharolobus solfataricus
W433C	kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 140fold, kcat/KM for 4-methylumbelliferyl beta-D-galactoside is reduced 230fold, kcat/KM for 4-methylumbelliferyl beta-D-xyloside is reduced 14fold, kcat/KM for 4-methylumbelliferyl beta-D-fucoside is reduced 138fold, kcat/KM for 4-methylumbelliferyl beta-D-mannoside is reduced 16fold	Saccharolobus solfataricus
W433C	the kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 140fold, and 4-methylumbelliferyl beta-D-galactoside is reduced 230fold. Although the activities with 4-methylumbelliferyl beta-D-mannoside and 4-methylumbelliferyl beta-D-xyloside are also reduced in the mutant, this is to a much smaller extent. The mutant enzyme shows remarkably broader specificity than wild-type enzyme	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the activities of the wild-type enzyme in 30% acetonitrile, butanone and acetone are higher than those in dimethyl sulfoxide, dimethylformamide and 1,4-dioxane. The low boiling point of acetone limits the application of this solvent in thermophilic enzyme reactions	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.011	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.011	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.036	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.036	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.046	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.046	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.066	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.066	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.13	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.13	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.15	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
0.15	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
0.18	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.21	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
0.21	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
0.34	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.34	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.34	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.34	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.41	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.41	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.47	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.47	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.51	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.51	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.57	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
0.57	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
0.59	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.59	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.81	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.81	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.9	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.9	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
1.26	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
1.26	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
1.3	-	4-methylumbelliferyl beta-D-glucuronide	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
1.3	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
1.61	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
1.61	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
2.2	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
2.2	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
2.72	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
3.46	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
5.1	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
5.1	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
5.28	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
5.28	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
13.7	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
13.7	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
18.6	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
18.6	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
22.1	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
22.1	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
24.2	-	4-nitrophenyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
24.2	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P22498	-	-
Saccharolobus solfataricus P2	P22498	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-methylumbelliferyl beta-D-fucoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferol + D-fucose	-	?
4-methylumbelliferyl beta-D-fucoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-fucose	-	?
4-methylumbelliferyl beta-D-galactoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-galactose	-	?
4-methylumbelliferyl beta-D-galactoside + H2O	-	Saccharolobus solfataricus P2	4-methylumbelliferone + beta-D-galactose	-	?
4-methylumbelliferyl beta-D-galacturonate + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-galacturonate	-	?
4-methylumbelliferyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-glucose	-	?
4-methylumbelliferyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus P2	4-methylumbelliferone + beta-D-glucose	-	?
4-methylumbelliferyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferol + D-glucose	-	?
4-methylumbelliferyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus P2	4-methylumbelliferol + D-glucose	-	?
4-methylumbelliferyl beta-D-glucuronide + H2O	-	Saccharolobus solfataricus	4-methylumbelliferol + D-glucose	-	?
4-methylumbelliferyl beta-D-mannoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-mannose	-	?
4-methylumbelliferyl beta-D-mannoside + H2O	-	Saccharolobus solfataricus P2	4-methylumbelliferone + beta-D-mannose	-	?
4-methylumbelliferyl beta-D-mannoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferol + D-mannose	-	?
4-methylumbelliferyl beta-D-xyloside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferol + D-xylose	-	?
4-methylumbelliferyl beta-D-xyloside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferone + beta-D-xylose	-	?
4-nitrophenyl beta-D-fucoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + D-fucose	-	?
4-nitrophenyl beta-D-galactoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + beta-D-galactose	-	?
4-nitrophenyl beta-D-galactoside + H2O	-	Saccharolobus solfataricus P2	4-nitrophenol + beta-D-galactose	-	?
4-nitrophenyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus	4-nitrophenyl + D-glucose	-	?
4-nitrophenyl beta-D-glucuronide + H2O	-	Saccharolobus solfataricus	4-nitrophenol + D-glucose	-	?
4-nitrophenyl beta-D-mannoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + D-mannose	-	?
4-nitrophenyl beta-D-xyloside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + D-xylose	-	?

Synonyms

Synonyms	Comment	Organism
LACS	-	Saccharolobus solfataricus
SsbetaG	-	Saccharolobus solfataricus
SSO3019	locus name	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.004	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.004	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.018	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.018	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.028	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.028	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.047	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.047	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.08	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.08	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.81	-	4-methylumbelliferyl beta-D-glucuronide	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.81	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.92	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.92	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
1.5	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
1.5	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
1.8	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
1.8	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
2.65	-	4-nitrophenyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
2.65	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
2.72	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
2.72	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
2.8	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
2.8	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
2.8	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
2.8	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
3.8	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
3.8	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
3.95	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
3.95	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
4.35	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
4.35	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
5.1	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
5.1	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
5.16	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
5.16	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
5.4	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
5.4	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
7.2	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
7.2	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
9.43	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
9.43	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
14.6	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
14.6	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
18	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
18	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
31	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
31	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
33	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
33	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
80	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
89	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
98	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
98	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
140	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
140	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.0006	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.001	-	4-nitrophenyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.001	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.001	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.0013	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.002	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.002	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.0022	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.0025	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.003	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.003	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.0032	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.005	-	4-nitrophenyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.005	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.0051	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.0063	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.011	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.015	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.015	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.015	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.02	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.03	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.036	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.036	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.05	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
0.053	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, mutant enzyme E432C	Saccharolobus solfataricus
0.058	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.058	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.06	-	4-methylumbelliferyl beta-D-galacturonate	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
0.068	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.068	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, mutant enzyme E432C	Saccharolobus solfataricus
0.076	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.11	-	4-nitrophenyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
0.11	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
0.123	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.123	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.506	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.506	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, mutant enzyme W433C	Saccharolobus solfataricus
0.6	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
0.6	-	4-methylumbelliferyl beta-D-glucuronide	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
0.77	-	4-nitrophenyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
0.77	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
1.49	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
2.2	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
2.5	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
2.9	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
3.2	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
5.1	-	4-methylumbelliferyl beta-D-glucuronide	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
6.3	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
7.3	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
11	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
12.7	-	4-nitrophenyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
12.7	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
15	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
20	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
29.8	-	4-nitrophenyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
29.8	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
30	-	4-methylumbelliferyl beta-D-xyloside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
42.6	-	4-nitrophenyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme	Saccharolobus solfataricus
42.6	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5, 45°C, wild-type enzyme	Saccharolobus solfataricus
50	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5, 80°C, wild-type enzyme	Saccharolobus solfataricus
53	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C	Saccharolobus solfataricus
76	-	4-methylumbelliferyl beta-D-mannoside	pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C	Saccharolobus solfataricus
1490	-	4-methylumbelliferyl beta-D-galactoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
2900	-	4-methylumbelliferyl beta-D-glucoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus
7300	-	4-methylumbelliferyl beta-D-fucoside	pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme	Saccharolobus solfataricus