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Information on EC 3.2.1.6 - endo-1,3(4)-beta-glucanase
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EC Tree
3.2.1.6 endo-1,3(4)-beta-glucanaseIUBMB Comments
Substrates include laminarin, lichenin and cereal D-glucans; different from EC 3.2.1.52 beta-N-acetylhexosaminidase.
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Word Map
- 3.2.1.6
- cellulase
- xylanase
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cellulolytic
-
carboxymethyl
- straw
- trichoderma
- carboxymethylcellulase
- beta-glucosidase
-
fpase
- beta-glucans
- thermocellum
-
lignocellulosic
- xylan
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saccharification
- avicel
- lichenan
- reesei
- hemicellulose
- avicelase
- endoglucanases
- bagasse
-
cellulosic
- bran
- cellobiohydrolase
-
cellulose-binding
- cellulomonas
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cellulose-degrading
- exoglucanase
- fibrobacter
-
lignocellulolytic
- cellobiase
- cellulosome
- succinogenes
- cellooligosaccharides
- cellotriose
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mixed-linkage
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xylanolytic
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microcrystalline
- cellotetraose
- agriculture
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fibrolytic
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acid-swollen
- cellopentaose
- synthesis
- food industry
- drug development
- degradation
- nutrition
- analysis
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lignocellulose-degrading
- macerans
- laminaribiose
- neocallimastix
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dockerin
- biotechnology
- sawdust
- endo-1,4-beta-xylanase
- endo-1,4-beta-d-glucanase
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
cmcase, lichenase, endo-beta-1,4-glucanase, endo-1,4-beta-glucanase, beta-1,3-1,4-glucanase, cel8a, h(a16-m), lam16a, fsbeta-glucanase, endo-beta-1,3-1,4-glucanase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AFUA_1G05290
AFUA_2G14360
AFUA_4G13360
AFUA_5G02280
beta-1,3-1,4-glucanase
beta-1,3-glucanase
beta-D-(1-3)-glucanase
beta-glucanase
bglBC1
CC1G_01665
Cel8A
CelL
enda-beta-(1-3),(1-4)-glucanase
endo-1,3(4)-beta-glucanase
endo-1,3-1,4-beta-D-glucanase
endo-1,3-beta-D-glucanase
endo-1,3-beta-glucanase
endo-1,4-beta-glucanase
endo-beta-(1-3)-D-glucanase
endo-beta-1,3(4)-glucanase
endo-beta-1,3-1,4-glucanase
endo-beta-1,3-glucanase
endo-beta-1,4-glucanase
ENG16A
Eng2
Eng3
Eng4
Eng5
GluA
H(A16-M)
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1,3-1,4-beta-glucanase from Bacillus macerans in which the 16 N-terminal residues have been replaced by the respective residues of the enzyme from Bacillus amyloliquefaciens
Lam16A
lamarinase
laminaranase
laminarinase
LamR
TeEgl5A
Xyn10B
additional information
Xyn10B
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modular enzyme composed of two family-22 carbohydrate-binding modules, a family-9 carbohydrate-binding module, and two S-layer homologous modules
Xyn10B
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modular enzyme comprising two family-22 carbohydrate-binding modules (CBMs), a family-10 catalytic module of glycoside hydrolases, a family-9 carbohydrate-binding module, and two S-layer homologous modules consecutively from the N-terminus
additional information
the bacterial beta-1,3-1,4-glucanase belongs to the glycosyl hydrolase family 16, GH16, possessing the conserved EIDIEF motif
additional information
the bacterial beta-1,3-1,4-glucanase belongs to the glycosyl hydrolase family 16, GH16, possessing the conserved EIDIEF motif
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additional information
-
HdLam33 belongs to the glycoside hydrolase family 16, GH16
additional information
-
the enzyme belongs to the glycosyl hydrolase family 81, GH81
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5
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-
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(Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5
reaction is not in rapid equilibrium
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(Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5
substrates include laminarin, lichenin, and cereal D-glucans, different from EC 3.2.1.52 beta-L-N-acetylhexosaminidase
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(Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5
substrates include laminarin, lichenin, and cereal D-glucans, different from EC 3.2.1.52 beta-L-N-acetylhexosaminidase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
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SYSTEMATIC NAME
IUBMB Comments
3(or 4)-beta-D-glucan 3(4)-glucanohydrolase
Substrates include laminarin, lichenin and cereal D-glucans; different from EC 3.2.1.52 beta-N-acetylhexosaminidase.
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CAS REGISTRY NUMBER
COMMENTARY
62213-14-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,3-1,4-beta-D-glucan + H2O
?
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LamR cleaves the 1,4-beta-linkages of 3-O-substituted beta-glucose in beta-glucans such as lichenin and 1,3-1,4-beta-glucan from the cell walls of barley endosperm, analysis of reaction products
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-
?
alpha-laminariheptaosyl fluoride
?
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substrate can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. NMR confirms uniquely beta-1,3-linkages and no reducing end. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima
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?
beta-1,3-1,4-D-glucotriose + H2O
D-glucose + cellobiose
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-
-
?
beta-Glc-(1->3)-beta-Glc-(1->4)-beta-Glc-(1->3)-beta-Glc-(1->4)-Glc + H2O
beta-Glc-(1->3)-beta-Glc-(1->4)-beta-Glc + ?
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main product, plus some tetrasaccharide. The enzyme acts on both beta-1,4-linkages of substrate
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?
carboxymethyl-cellulose + H2O
?
15% of the activity with beta-D-glucan
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-
?
cellodextrin + H2O
cellobiose + cellotriose + higher oligosaccharides
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-
-
?
curdlan + H2O
laminaribiose + laminaritriose + ?
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main products
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?
Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl + H2O
methylumbelliferone + Glc-beta(1,4)-Glc-beta(1,3)-Glc
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-
-
-
?
konjac glucomannan + H2O
?
40% of the activity with barley beta-1,3-1,4-D-glucan
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-
?
laminarihexaose + H2O
D-glucose + laminaribiose + laminaritriose
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-
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?
laminarin + H2O
6-O-glucosyl-laminaritriose + ?
6-O-glucosyl-laminaritriose is produced as one of the major products
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-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
laminarin + H2O
D-glucose + laminaripentaose
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from Laminaria digitata, best substrate for LIC 1, cleaves internal bonds, LIC 1 may have five subsites for glucose at the aglycone site and at least one subsite in the glucone site, LIC 1 should move along the substrate 1 or 5 glucosyl residues after each hydrolytic step, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction
LIC 1 forms almost only glucose and laminaripentaose as soluble products, laminaripentaose is the main product, glucose is 20% from total products and 25% from soluble products
-
?
laminarin + H2O
laminaritriose
from Laminaria digitata
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-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose
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-
-
?
lichenan + H2O
4-O-glucosyl-laminaribiose + ?
4-O-glucosyl-laminaribiose is produced as one of the major products
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-
?
lichenan + H2O
beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-D-glucopyranose + ?
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-
-
-
?
lichenan + H2O
beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranose + ?
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from Cetraria islandica. HdLam33 produces no cellotriose, cellobiose, and glucose from lichenan, but produced the trisaccharide cellobiosyl-beta-1,3-glucose
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-
?
lichenin + H2O
?
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LamR cleaves the 1,4-beta-linkages of 3-O-substituted beta-glucose in beta-glucans such as lichenin, analysis of reaction products
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-
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
lichenin + H2O
cellotetraose + cellopentaose + glucose
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from Cetraria islandica, cleaves internal bonds, LIC 1 has high affinity for sequences of cellotetraose linked by beta-1,3-links releasing cellotetraose from lichenin, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction
main products are cellotetraose and cellopentaose, glucose is only 1% of soluble products, LIC 1 produces an additional saccharide as product possibly containing beta-1,3- and beta-1,4-linkages
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?
oxidized laminarin
laminaritetraose + laminaritriose + laminaribiose + D-glucose
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-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
D-glucose + p-nitrophenol
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slight activity
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?
polysaccharides + H2O
oligosaccharides
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role in maintenance of channels for communication and translocation through cell walls
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?
1,3-1,4-beta-D-glucan + H2O
cellooligosaccharide
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soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan
spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation
-
?
1,3-1,4-beta-D-glucan + H2O
cellooligosaccharide
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soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan
spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation
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?