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Literature summary for 3.2.1.6 extracted from

  • Cheng, Y.M.; Hong, T.Y.; Liu, C.C.; Meng, M.
    Cloning and functional characterization of a complex endo-beta-1,3-glucanase from Paenibacillus sp. (2009), Appl. Microbiol. Biotechnol., 81, 1051-1061.
    View publication on PubMed
Search for more literature for 3.2.1.6

Application

Application Comment Organism
drug development the various truncated proteins of LamA also show inhibition on the mycelium growth of Rhizopus solani, a pathogen infecting a broad range of many crops, suggesting the application potential of LamA as an antifungal agent in many aspects Paenibacillus sp. CCRC 17245

Cloned(Commentary)

Cloned (Comment) Organism
expression of five truncated proteins of LamA in Escherichia coli BL21(DE3) Paenibacillus sp. CCRC 17245

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Laminarin 1.1 mg/ml, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 Laminarin 1.8 mg/ml, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 Laminarin 2.4 mg/ml, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 Laminarin 2.4 mg/ml, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 Laminarin 2.9 mg/ml, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38200
-
 calculated molecular mass of expressed C of LamA Paenibacillus sp. CCRC 17245
51600
-
 calculated molecular mass of expressed CF of LamA Paenibacillus sp. CCRC 17245
77000
-
 calculated molecular mass of expressed SC of LamA Paenibacillus sp. CCRC 17245
92000
-
 calculated molecular mass of expressed CB3 of LamA Paenibacillus sp. CCRC 17245
144600
-
 calculated molecular mass of expressed CBF of LamA Paenibacillus sp. CCRC 17245

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. CCRC 17245 Q000P7
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized Ni2+ affinity chromatography, gel filtration chromatography Paenibacillus sp. CCRC 17245

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.3 U/nmol with substrate curdlan, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.3 U/nmol with substrate pachyman, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.4 U/nmol with substrate barley beta-glucan, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.4 U/nmol with substrate lichenan, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.4 U/nmol with substrate lichenan, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.4 U/nmol with substrate pachyman, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.4 U/nmol with substrate zymosan A, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.5 U/nmol with substrate barley beta-glucan, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.5 U/nmol with substrate zymosan A, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.6 U/nmol with substrate curdlan, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.6 U/nmol with substrate pachyman, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.7 U/nmol with substrate barley beta-glucan, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.7 U/nmol with substrate curdlan, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.7 U/nmol with substrate lichenan, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.7 U/nmol with substrate zymosan A, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.8 U/nmol with substrate pachyman, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.8 U/nmol with substrate zymosan A, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.9 U/nmol with substrate barley beta-glucan, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 0.9 U/nmol with substrate lichenan, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.0 U/nmol with substrate curdlan, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.3 U/nmol with substrate lichenan, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.3 U/nmol with substrate pachyman, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.4 U/nmol with substrate laminarin, truncated protein C of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.5 U/nmol with substrate barley beta-glucan, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.6 U/nmol with substrate zymosan A, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 1.7 U/nmol with substrate laminarin, truncated protein CF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 2.0 U/nmol with substrate curdlan, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 2.6 U/nmol with substrate laminarin, truncated protein CB3 of LamA Paenibacillus sp. CCRC 17245
additional information
-
 3.7 U/nmol with substrate laminarin, truncated protein CBF of LamA Paenibacillus sp. CCRC 17245
additional information
-
 4.6 U/nmol with substrate laminarin, truncated protein SC of LamA Paenibacillus sp. CCRC 17245
additional information
-
 no activity with carboxymethylcellulose and xylan of all truncated proteins of LamA Paenibacillus sp. CCRC 17245

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
curdlan + H2O from Alcaligenes faecalis Paenibacillus sp. CCRC 17245 ?
-
 ?
laminarin + H2O from Laminaria digitata Paenibacillus sp. CCRC 17245 laminaritriose
-
 ?
lichenan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
additional information no activity with carboxymethylcellulose and xylan of all truncated proteins of LamA Paenibacillus sp. CCRC 17245 ?
-
 ?
pachyman + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
zymosan A + H2O from Saccharomyces cerevisiae Paenibacillus sp. CCRC 17245 ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-1,3-beta-glucanase
-
 Paenibacillus sp. CCRC 17245
LamA
-
 Paenibacillus sp. CCRC 17245
laminarinase
-
 Paenibacillus sp. CCRC 17245

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 for truncated protein CBF, pH 5.5 Paenibacillus sp. CCRC 17245
50
-
 for truncated protein C, pH 5.5 Paenibacillus sp. CCRC 17245

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 toward laminarin hydrolysis for truncated proteins CBF and C Paenibacillus sp. CCRC 17245