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Literature summary for 3.2.1.6 extracted from

  • Money, V.A.; Cartmell, A.; Guerreiro, C.I.; Ducros, V.M.; Fontes, C.M.; Gilbert, H.J.; Davies, G.J.
    Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants (2008), Org. Biomol. Chem., 6, 851-853.
    View publication on PubMed
Search for more literature for 3.2.1.6

Crystallization (Commentary)

Crystallization (Comment) Organism
3-D structure, at 1.5 A resolution, of CtLic26A in complex with thiopentasaccharide. Crystals are in space group P2(1)2(1)2(1) with cell dimensions of a = 49.3 A, b = 63.0 A and c = 78.3 A and with a single molecule of CtLic26A in the asymmetric unit Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
E258A kcat/Km for beta-glucan is 4.8fold lower than wild-type value Acetivibrio thermocellus
E70A kcat/Km for beta-glucan is 36.5fold lower than wild-type value Acetivibrio thermocellus
F41A kcat/Km for beta-glucan is 219fold lower than wild-type value Acetivibrio thermocellus
K260A kcat/Km for beta-glucan is 31fold lower than wild-type value Acetivibrio thermocellus
N157A kcat/Km for beta-glucan is 1.4fold lower than wild-type value Acetivibrio thermocellus
Q18A kcat/Km for beta-glucan is 6.25fold lower than wild-type value Acetivibrio thermocellus
W114A kcat/Km for beta-glucan is 3.5fold lower than wild-type value Acetivibrio thermocellus
W72A kcat/Km for beta-glucan is 1.3fold higher than wild-type value Acetivibrio thermocellus
Y115A kcat/Km for beta-glucan is 14.6fold lower than wild-type value Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for beta-glucan is 0.34 mg/ml for wild-type enzyme, 0.67 mg/ml for mutant enzyme Q18A, 1.3 mg/ml for mutant enzymes F41A and E70A, 0.46 mg/ml for mutant enzyme W72A, 1.4 mg/ml for mutant enzyme W114A, 1 mg/ml for mutant enzyme Y115A, 0.49 mg/ml for mutant enzyme N157A, 0.55 mg/ml for mutant enzyme E258A, 0.65 mg/ml for mutant enzyme K260A Acetivibrio thermocellus
0.011
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme N157A Acetivibrio thermocellus
0.11
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl wild-type enzyme Acetivibrio thermocellus
0.139
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme F41A Acetivibrio thermocellus
0.155
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme E70A Acetivibrio thermocellus
0.188
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme E258A Acetivibrio thermocellus
0.26
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme Y115A Acetivibrio thermocellus
1.162
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme K260A Acetivibrio thermocellus
1.763
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme Q18A Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl + H2O
-
 Acetivibrio thermocellus methylumbelliferone + Glc-beta(1,4)-Glc-beta(1,3)-Glc
-
 ?

Synonyms

Synonyms Comment Organism
beta-1,3:1,4 glucanase
-
 Acetivibrio thermocellus
CtLic26A
-
 Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000017
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme F41A Acetivibrio thermocellus
0.000083
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme E70A Acetivibrio thermocellus
0.00083
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme Y115A Acetivibrio thermocellus
0.012
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme N157A Acetivibrio thermocellus
0.027
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme E258A Acetivibrio thermocellus
0.035
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme Q18A Acetivibrio thermocellus
0.053
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl mutant enzyme K260A Acetivibrio thermocellus
0.383
-
 Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl wi.d-type enzyme Acetivibrio thermocellus
26.7
-
 beta-Glucan mutant enzyme F41A Acetivibrio thermocellus
83
-
 beta-Glucan mutant enzyme K260A Acetivibrio thermocellus
135
-
 beta-Glucan mutant enzyme E70A Acetivibrio thermocellus
250
-
 beta-Glucan mutant enzyme Y115A Acetivibrio thermocellus
383
-
 beta-Glucan mutant enzyme Q18A Acetivibrio thermocellus
433
-
 beta-Glucan mutant enzyme E258A Acetivibrio thermocellus
1233
-
 beta-Glucan wild-type enzyme Acetivibrio thermocellus
1300
-
 beta-Glucan mutant enzyme N157A Acetivibrio thermocellus
1417
-
 beta-Glucan mutant enzyme W114A Acetivibrio thermocellus
2250
-
 beta-Glucan mutant enzyme W72A Acetivibrio thermocellus