Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1%, activates | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris. The beta-1,3-1,4-glucanase gene is modified and expressed according to its preferred codon usage | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM | Bacillus licheniformis | |
Zn2+ | 10 mM | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Bacillus licheniformis | |
Fe2+ | activates | Bacillus licheniformis | |
Mn2+ | activates | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q8GMY0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
lichenan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
additional information | no activity on cellulose | Bacillus licheniformis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, 5% loss of activity | Bacillus licheniformis |
60 | - |
30 min, 40% loss of activity | Bacillus licheniformis |
70 | - |
30 min, complete loss of activity | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: abput 40% of maximal activity, pH 8.0: about 55% of maximal activity | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
12 h, enzyme retains 80% of its original activity | Bacillus licheniformis |
4 | 6 | stable | Bacillus licheniformis |
8 | - |
12 h, enzyme retains 80% of its original activity | Bacillus licheniformis |