EC Number   |
General Information |
Reference |
|---|
   3.2.1.6 | evolution |
the enzyme belongs to glycoside hydrolase family 8 (GH8) and contains A154TDGDMDIAYSLLLADKQW172. The enzyme has a lower ratio of lichenase/barley-beta-D-glucanase activities compared to glycoside hydrolase family GH16 enzymes |
729478 |
| 3.2.1.6 | metabolism |
enzyme is highly resistant to trypsin, alpha-chymotrypsin, collagenase and Neutrase 0.8L |
713878 |
| 3.2.1.6 | more |
active site residues are Trp103, Asp104, Ile106, Ile108 and Glu109 in the beta-strand. Asp107 and Glu105 (as nucleophile) and Glu109 (as acid catalyst) are essential for enzyme activity. Molecular dynamics simulations and structure-function analysis, three-dimensional model, overview |
729343 |
| 3.2.1.6 | more |
Glu95 and Asp156 are catalytically active residues |
729478 |
| 3.2.1.6 | more |
modeled enzyme exhibits a (beta/alpha)8-barrel structure typical of GH5 members with an extra one-turn helix. One putative disulfide bridge might form to stabilize the loops |
-, 729084 |
| 3.2.1.6 | more |
the enzyme has an active site of 13 amino acids typical of glucanase with beta-1,3 and 1,4 action mode, comparative molecular modelling, overview. The enzyme's active site is composed of 13 amino acids, Trp93, Leu94, Lys95, Asp96, His97, Gly98, Pro99, Asp100, Lys101, Gly102, Phe103, Glu104, Asp105, a space of 2831 A, and presents alpha-helices and loop structures |
730361 |
| 3.2.1.6 | physiological function |
a mutant lacking the activities of GH81 enzyme Eng1 and the activities of GH16 isoforms Eng2 to Eng5 shows conidial defects, with linear chains of conidia unable to separate while the germination rate is not affected. The mutant shows a about 51% decrease in the endo-beta-(1,3)-glucanase activity in in dormant conidia, about 74% in swollen morphotype and about 32% in germinating conidia |
-, 738012 |
| 3.2.1.6 | physiological function |
the enzyme causes hydrolysis of the membrane of hyphae from Moniliophthora perniciosa. Recombinant beta-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa strain ALF553 mycelial growth |
730361 |
| 3.2.1.6 | physiological function |
When Schizosaccharomyces pombe diploid cells undergo meiosis, they differentiate into asci containing four haploid ascospores that are highly resistant to environmental stress. beta-Glucanase Eng2 and endo-alpha-1,3-glucanase Agn2 are required for ascus wall endolysis after sporulation in Schizosaccharomyces pombe |
708354 |
