Protein Variants | Comment | Organism |
---|---|---|
N207D | increase in Tm-value to 75.3°C, compared to wild-type Tm-value of 71.4°C | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
hypothesis for thermal inactivation: the first phase in thermal denaturation could be produced when calcium interactions are disrupted and the main loop becomes free to rearrange. This phase yields a partially active intermediary during the first phase of inactivation and then the intermediary is slowly converted into a totally inactive enzyme in the second phase | Bacillus licheniformis |
71 | - |
Tm-value for wild-type enzyme is 71.4°C | Bacillus licheniformis |
75 | - |
Tm-value for mutant enzyme N207D is 75.3°C | Bacillus licheniformis |