General Stability | Organism |
---|---|
LIC 1 is stable in the presence of Periplaneta americana proteinases | Periplaneta americana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carbodiimide | - |
Periplaneta americana | |
additional information | not inhibited by laminarin or lichenin | Periplaneta americana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Periplaneta americana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Periplaneta americana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Periplaneta americana | LIC 1 lyses plant and fungal cell walls, making available their contents, LIC 1 may help the cellulases CEL 1 and CEL 2 by hydrolyzing beta-1,3-1,4-glucans, important chiefly in cereal walls | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Periplaneta americana | - |
adult, female | - |
Purification (Comment) | Organism |
---|---|
from midgut and salivary gland | Periplaneta americana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5 | reaction is not in rapid equilibrium | Periplaneta americana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut | - |
Periplaneta americana | - |
salivary gland | LIC 1 is secreted by the salivary glands | Periplaneta americana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | from Laminaria digitata, best substrate for LIC 1, cleaves internal bonds, LIC 1 may have five subsites for glucose at the aglycone site and at least one subsite in the glucone site, LIC 1 should move along the substrate 1 or 5 glucosyl residues after each hydrolytic step, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction | Periplaneta americana | D-glucose + laminaripentaose | LIC 1 forms almost only glucose and laminaripentaose as soluble products, laminaripentaose is the main product, glucose is 20% from total products and 25% from soluble products | ? | |
lichenin + H2O | from Cetraria islandica, cleaves internal bonds, LIC 1 has high affinity for sequences of cellotetraose linked by beta-1,3-links releasing cellotetraose from lichenin, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction | Periplaneta americana | cellotetraose + cellopentaose + glucose | main products are cellotetraose and cellopentaose, glucose is only 1% of soluble products, LIC 1 produces an additional saccharide as product possibly containing beta-1,3- and beta-1,4-linkages | ? | |
additional information | LIC 1 lyses plant and fungal cell walls, making available their contents, LIC 1 may help the cellulases CEL 1 and CEL 2 by hydrolyzing beta-1,3-1,4-glucans, important chiefly in cereal walls | Periplaneta americana | ? | - |
? | |
additional information | a group in LIC 1 with pK of 4.9 is necessary for substrate binding | Periplaneta americana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Periplaneta americana |
Synonyms | Comment | Organism |
---|---|---|
LIC 1 | - |
Periplaneta americana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Periplaneta americana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
750 | - |
Laminarin | pH 6, 30°C | Periplaneta americana | |
1050 | - |
lichenin | pH 6, 30°C | Periplaneta americana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
around | Periplaneta americana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | stable in the range | Periplaneta americana |