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Literature summary for 3.2.1.6 extracted from

  • Genta, F.A.; Terra, W.R.; Ferreira, C.
    Action pattern, specificity, lytic activities, and physiological role of five digestive beta-glucanases isolated from Periplaneta americana (2003), Insect Biochem. Mol. Biol., 33, 1085-1097.
    View publication on PubMed

General Stability

General Stability Organism
LIC 1 is stable in the presence of Periplaneta americana proteinases Periplaneta americana

Inhibitors

Inhibitors Comment Organism Structure
carbodiimide
-
Periplaneta americana
additional information not inhibited by laminarin or lichenin Periplaneta americana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic data Periplaneta americana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Periplaneta americana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Periplaneta americana LIC 1 lyses plant and fungal cell walls, making available their contents, LIC 1 may help the cellulases CEL 1 and CEL 2 by hydrolyzing beta-1,3-1,4-glucans, important chiefly in cereal walls ?
-
?

Organism

Organism UniProt Comment Textmining
Periplaneta americana
-
adult, female
-

Purification (Commentary)

Purification (Comment) Organism
from midgut and salivary gland Periplaneta americana

Reaction

Reaction Comment Organism Reaction ID
(Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5 reaction is not in rapid equilibrium Periplaneta americana

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
Periplaneta americana
-
salivary gland LIC 1 is secreted by the salivary glands Periplaneta americana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O from Laminaria digitata, best substrate for LIC 1, cleaves internal bonds, LIC 1 may have five subsites for glucose at the aglycone site and at least one subsite in the glucone site, LIC 1 should move along the substrate 1 or 5 glucosyl residues after each hydrolytic step, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction Periplaneta americana D-glucose + laminaripentaose LIC 1 forms almost only glucose and laminaripentaose as soluble products, laminaripentaose is the main product, glucose is 20% from total products and 25% from soluble products ?
lichenin + H2O from Cetraria islandica, cleaves internal bonds, LIC 1 has high affinity for sequences of cellotetraose linked by beta-1,3-links releasing cellotetraose from lichenin, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction Periplaneta americana cellotetraose + cellopentaose + glucose main products are cellotetraose and cellopentaose, glucose is only 1% of soluble products, LIC 1 produces an additional saccharide as product possibly containing beta-1,3- and beta-1,4-linkages ?
additional information LIC 1 lyses plant and fungal cell walls, making available their contents, LIC 1 may help the cellulases CEL 1 and CEL 2 by hydrolyzing beta-1,3-1,4-glucans, important chiefly in cereal walls Periplaneta americana ?
-
?
additional information a group in LIC 1 with pK of 4.9 is necessary for substrate binding Periplaneta americana ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Periplaneta americana

Synonyms

Synonyms Comment Organism
LIC 1
-
Periplaneta americana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Periplaneta americana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
750
-
Laminarin pH 6, 30°C Periplaneta americana
1050
-
lichenin pH 6, 30°C Periplaneta americana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
around Periplaneta americana

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable in the range Periplaneta americana