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Literature summary for 3.2.1.6 extracted from
- Characterization of a thermostable endo-1,3(4)-beta-glucanase from Caldicellulosiruptor sp. strain F32 and its application for yeast lysis (2016), Appl. Microbiol. Biotechnol., 100, 4923-4934 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caldicellulosiruptor sp. F32 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 10 mM, 77% residual activity | Caldicellulosiruptor sp. F32 |  |
| EDTA | 10 mM, 63% residual activity | Caldicellulosiruptor sp. F32 | |
| Mn2+ | 1 mM, 56% of initial activity | Caldicellulosiruptor sp. F32 | |
| SDS | 10 mM, 70% residual activity | Caldicellulosiruptor sp. F32 | |
| Urea | 10 mM, 85% residual activity | Caldicellulosiruptor sp. F32 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Barley beta-glucan | Km value 1.38 mg/ml, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 | |
| additional information | - |
Laminarin | Km value 2.63 mg/ml, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 119% of initial activity | Caldicellulosiruptor sp. F32 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor sp. F32 | A0A0S2UQ60 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Caldicellulosiruptor sp. F32 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 172 | - |
substrate laminarin, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 |
| 2961 | - |
substrate barley beta-glucan, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Caldicellulosiruptor sp. F32 | ? | - |
? | |
| beta-1,3-1,4-D-glucotriose + H2O | - |
Caldicellulosiruptor sp. F32 | D-glucose + cellobiose | - |
? | |
| laminarin + H2O | - |
Caldicellulosiruptor sp. F32 | ? | - |
? | |
| additional information | enzyme hydrolyzes both beta-1,3-glucan (laminarin) and beta-1,3-1,4-glucan linkages, catalyzes endo-hydrolysis of the beta-1,4 glycosidic linkage adjacent to a 3-O-substituted glucosyl residues in the mixed linked beta-glucans, as well as the beta-1,3 linkage. No substrates: carboxymethylcellulose, avicel | Caldicellulosiruptor sp. F32 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lam16A | - |
Caldicellulosiruptor sp. F32 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Caldicellulosiruptor sp. F32 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
half-life 45 h | Caldicellulosiruptor sp. F32 |
| 85 | - |
30 min, 20% residual activity | Caldicellulosiruptor sp. F32 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Caldicellulosiruptor sp. F32 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | kcat/Km value 102 ml/mg/s, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 | |
| additional information | - |
Barley beta-glucan | kcat/Km value 2116 ml/mg/s, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 | |
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